A Comparative Transcriptomics Approach to Analyzing the Differences in Cold Resistance in Pomacea canaliculata between Guangdong and Hunan

Abstract

Pomacea canaliculata, known as an invasive freshwater snail, is also called a golden apple snail; its survival and expansion are greatly affected by temperature. In this study, high-throughput sequencing (RNA-seq) was used to perform comparative transcriptome analysis on the muscular tissue (G_M) of snails in Guangdong and Hunan. Differential gene screening was performed with FDR <0.05 and |log2FoldChange| >1 as the threshold, and a total of 1,368 differential genes were obtained (671 genes showed upregulation in snails from Guangdong, and 697 genes displayed upregulation in snails from Hunan). Fifteen genes were identified as candidate genes for the cold hardiness of Pomacea canaliculata. Among them, three genes were involved in energy metabolism (glycogen synthase, 1; DGK, 1; G6PD, 1); seven genes were involved in homeostasis regulation (HSP70, 2; BIP, 1; GPX, 1; GSTO 1, G6PD, 1; caspase-9, 1); two genes were involved in amino acid metabolism (glutamine synthetase, 1; PDK, 1); and four genes were involved in membrane metabolism (inositol-3-phosphate synthase, 1; Na+/K+-ATPase, 1; calcium-binding protein, 2). This study presents the molecular mechanisms for the cold hardiness of Pomacea canaliculata, which could provide a scientific basis for the forecast and prevention of harm from Pomacea canaliculata.

Predicting Current Potential Distribution and the Range Dynamics of Pomacea canaliculata in China under Global Climate Change

“…While no eggs can overwinter, adults and juveniles may survive and contribute to the next reproductive season [ 45 ]. Meanwhile, P. canaliculata could boost the energy supply, tolerance ability, and supercooling to improve the cold resistance [ 46 ]. This could well explain why P. canaliculata has been found numerous times in Beijing, Shandong Province, and other northern Chinese cities in recent years.…”

Predicting the Potential Geographic Distribution of Invasive Freshwater Apple Snail Pomacea canaliculate (Lamarck, 1819) under Climate Change Based on Biomod2

“…Yoshida (2009) [66] has demonstrated that although P. canaliculata eggs cannot overwinter in extremely low-temperature conditions, they can endure cold winters and resume reproduction when temperatures rise in the following year. Furthermore, P. canaliculata can increase its energy supply and tolerance capacity, thereby enhancing its cold resistance [67]. For example, Wada and Matsukura (2007) [27] discovered that P. canaliculata in Ibaraki Prefecture, Japan, not only survived cold winters but also exhibited greater cold tolerance compared to P. canaliculata in southern Japan.…”

Identification and functional analysis of proteins in Pomacea canaliculate gut contents: a proteomics analysis

“…J Sun et al conducted mRNA expression of P. canaliculata in various areas and cold conditions using second-generation sequencing technology. Additionally, they also performed transcriptomic studies of the liver and ovaries [16][17][18][19][20]. The library has laid a foundation for the identi cation of functional proteins and the analysis of physiological mechanism of P. canaliculata.…”