A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus

Wang, Yan; Wang, Baojie; Shao, Xuqing; Liu, Mei; Jiang, Keyong; Wang, Mengqiang; Wang, Lei

doi:10.1007/s00438-019-01621-4

Cited by 12 publications

(5 citation statements)

References 51 publications

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“…Ten genes are identified: all are putatively implicated in developmental processes such as axon morphogenesis ( Glass ), eye formation via regulation of the initial specification of retinal cells ( Pph ; En ), and development or differentiation ( Notch ). The Hedgehog protein is believed to play an important role in one of the fundamental signal transduction pathways; its homeodomain contains sequence-specific DNA-binding proteins that act as regulators of transcription ( Wang et al 2020 ). During embryogenesis, morphogenic pathways such as WNT and Hedgehog are constitutively active; however, the activity of these pathways decreases in adulthood.…”

Section: Resultsmentioning

confidence: 99%

Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii

Calderón-Rosete¹,

González-Barrios²,

Piña-Leyva³

et al. 2021

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Crayfish serve as a model for studying the effect of environmental lighting on locomotor activity and neuroendocrine functions. The effects of light on this organism are mediated differentially by retinal and extraretinal photoreceptors located in the cerebroid ganglion and the pleonal nerve cord. However, some molecular aspects of the phototransduction cascade in the pleonal extraretinal photoreceptors remain unknown. In this study, transcriptome data from the pleonal nerve cord of the crayfish Procambarus clarkii (Girard,1852) were analyzed to identify transcripts that potentially interact with phototransduction process. The Illumina MiSeq System and the pipeline Phylogenetically Informed Annotation (PIA) were employed, which places uncharacterized genes into pre-calculated phylogenies of gene families. Here, for the first time 62 transcripts identified from the pleonal nerve cord that are related to light-interacting pathways are reported; they can be classified into the following 11 sets: 1) retinoid pathway in vertebrates and invertebrates, 2) photoreceptor specification, 3) rhabdomeric phototransduction, 4) opsins 5) ciliary phototransduction, 6) melanin synthesis, 7) pterin synthesis, 8) ommochrome synthesis, 9) heme synthesis, 10) diurnal clock, and 11) crystallins. Moreover, this analysis comparing the sequences located on the pleonal nerve cord to eyestalk sequences reported in other studies reveals 94–100% similarity between the 55 common proteins identified. These results show that both retinal and pleonal non-visual photoreceptors in the crayfish equally expressed the transcripts involved in light detection. Moreover, they suggest that the genes related to ocular and extraocular light perception in the crayfish P. clarkii use biosynthesis pathways and phototransduction cascades commons.

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Section: Resultsmentioning

confidence: 99%

Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii

Calderón-Rosete¹,

González-Barrios²,

Piña-Leyva³

et al. 2021

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“…Genes upregulated in the 2-4 cell stage embryos of E. sinensis include Nanos, which is associated with sex differentiation of the germline, as well as genes involved in segmentation such as segmentation protein paired, hunchback and bric-a-brac. Embryos at 20 and 27 days following fertilization were collected for transcriptomic analysis from the red claw crayfish Cherax quadricarinatus, demonstrating large morphological changes, with 20 day embryos corresponding with the formation of eye pigments and short abdominal appendages without segmentation and day 27 embryos corresponding to the pre-hatching stage as the body and appendages were segmented (Wang et al, 2020). Fundamental development genes were highly expressed in the day 20 embryos, including HtrA2, Caspase 8, hedgehog, and MAPKm (Wang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Embryos at 20 and 27 days following fertilization were collected for transcriptomic analysis from the red claw crayfish Cherax quadricarinatus, demonstrating large morphological changes, with 20 day embryos corresponding with the formation of eye pigments and short abdominal appendages without segmentation and day 27 embryos corresponding to the pre-hatching stage as the body and appendages were segmented (Wang et al, 2020). Fundamental development genes were highly expressed in the day 20 embryos, including HtrA2, Caspase 8, hedgehog, and MAPKm (Wang et al, 2020). Establishing a detailed P. ornatus embryo transcriptome would allow for genes associated with embryonal development to be mapped across embryogenesis and enable more accurate staging of the P. ornatus embryonal stages.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis and Time to Hatch Visual Prediction of Embryo Development in the Ornate Spiny Lobster (Panulirus ornatus)

et al. 2022

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The ability to conduct closed life-cycle culture of tropical spiny lobsters, Panulirus ornatus, at the Institute for Marine and Antarctic Studies (IMAS) provides a unique opportunity to investigate specific developmental stages during embryogenesis. The production of closed life-cycle lobsters provides access to embryos at defined time points, yet physical staging is challenging due to their small size and environmental factors impacting their development. A transcriptome comprising 11 distinct stages across the 30-day P. ornatus embryonal development period allowed the establishment of the most detailed transcriptomic library of embryogenesis across decapods. A series of key genes across the 11 stages of embryonal development were characterized. The expression of neuropeptides was reported across P. ornatus embryos, suggesting they are primarily active during the later stages of embryogenesis as the nervous system develops and the animal prepares to hatch. Gastrula-specific genes, nanos and brachyury, presented an expression profile indicating gastrulation occurs early in embryogenesis. In addition to the molecular tools used to characterize embryo development, certain developmental characteristics, such as eye spot development, provide a measurable indicator that can be visualised. Hatch prediction models based on visual characteristics were shown to be an accurate method to predict the timing of the hatch for P. ornatus embryos kept at 26°C. The combination of morphological and visual measures with transcriptomics can be used to further define and establish the groundwork for future characterisation and staging of P. ornatus embryogenesis.

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“…The red claw crayfish ( Cherax quadricarinatus , RC‐crayfish) is an emerging and important commercial species in several countries and is also a potential biological model in crustacean biology (Haubrock et al, 2021; Wang et al, 2020). RC‐crayfish has sufficient biological and commercial characteristics adequate for farming (Saoud et al, 2013; Villarreal & Peláez, 1999).…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementations modulate the physiological parameters, fatty acids profile and the growth of red claw crayfish (Cherax quadricarinatus)

Shehata

Alhoshy

Wang

et al. 2022

Animal Physiology Nutrition

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An optimal diet is an important factor for the proper growth and health of crustaceans. However, the regulation of antioxidant activity and non-specific immunity related to the consumption of feed additives has not been studied in RC-crayfish. Triplicate groups of 20 crayfish/tank (36.72 ± 0.70 g) fed with a basal diet and sixteen experimental diets that contained five feed additives with four grade levels (40, 160, 240 and 320 mg/kg vitamin E, 2, 4, 6 and 8 g/kg nucleotides, 2, 4, 6 and 8 g/kg Haematococcus pluvialis, 5, 10, 15 and 20 g/kg arachidonic acid and 2.5, 5, 10 and 15 g/kg yeast extract) on physiological parameters, fatty acids profile and growth of Cherax quadricarinatus for a period of 70 days by using orthogonal array method (L16 4 5 ). The results showed that the antioxidants activity in the haemolymph and hepatopancreas were both higher in crayfish fed with diets NO. 9 to 12 than others. Also, all the diets except diets NO. 13 to 16 showed lower free radicals contents than the control group. Similarly, significantly higher nonspecific immune parameters were observed in the hepatopancreas of crayfish supplementations than those fed a control diet. Biochemical parameters related to protein profile in haemolymph increased in diets NO. 9 to 12 and then decreased in control and diets NO. 13 to 16, while the highest biochemical parameters related to lipid profile except HDL-c contents in haemolymph were observed in crayfish fed the control diet. Fatty acid composition in the hepatopancreas, muscle and ovary of RC-crayfish was significantly influenced by using the combination of Vit E, NT, H. pluvialis and YP compared to the control group. Compared to all treatments, RCcrayfish fed with diets NO. 2 and 12 had significantly stimulated higher growth performance and feed utilisation. Overall, our results suggest that diets supplemented with Vit E level of 240 mg/kg, in combination with 8 g/kg NT, 4 g/kg, H. pluvialis, 5 g/kg ARA and 10 g/kg YP are the promising treatments to increase antioxidants activity, non-specific immune response, fatty acids composition and growth of RC-crayfish. However, high dietary supplementations level can reduce antioxidants activity, immunity and inhibit growth.

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A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus

Cited by 12 publications

References 51 publications

Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii

Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii

Transcriptomic Analysis and Time to Hatch Visual Prediction of Embryo Development in the Ornate Spiny Lobster (Panulirus ornatus)

Dietary supplementations modulate the physiological parameters, fatty acids profile and the growth of red claw crayfish (Cherax quadricarinatus)

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