A comparative study on the effect of blood collection tubes on stress oxidative markers

Bastin, Alireza; Fooladi, Saba; Doustimotlagh, Amir Hossein; Vakili, Sina; Aminizadeh, Amir Hashem; Faramarz, Sanaz; Shiri, Hamidreza; Nematollahi, Mohammad Hadi

doi:10.1371/journal.pone.0266567

Cited by 8 publications

(4 citation statements)

References 33 publications

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“…This method is based on the ability of the compound of interest to reduce ferric (Fe 3) ions to ferrous (Fe 2) ions in the presence of a TPTZ (Tripyridyl-s-Triazine) solution at low pH. The Fe 2+ TPTZ complex is a violet-colored complex with maximum absorption at a wavelength of 593 nm (24). A FRAP reagent was prepared by mixing acetate buffer (300 mM), iron chloride (20 mM), and TPTZ (10 mM) in a ratio of 10:1:1.…”

Section: Frap Methodsmentioning

confidence: 99%

Evaluation of Antioxidant Potential and Free Radical Scavenging Activity of Methanol Extract from Scrophularia striata

Shiri,

Karimpour,

Sattari

et al. 2023

ABI

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Objectives: The effectiveness of Scrophularia striata in controlling infections and promoting wound healing has been reported. This study aimed to investigate the antioxidant properties of the methanol extract from Scrophularia striata. Methods: Scrophularia striata, a perennial wild plant found in various temperate and tropical areas of Iran, underwent a methanol extraction process to obtain its active compounds. The antioxidant property of the methanol extract of Scrophularia striata was evaluated by quantifying the total antioxidant level, determining the total phenol content, and conducting DPPH radical scavenging assays. Results: As the extraction concentrations of Scrophularia striata increase, both the total antioxidant level and total phenol content rise dramatically. With the progression of time and increase in plant extract concentrations, the efficacy of DPPH radical scavenging also shows a corresponding enhancement. Moreover, the IC50% value of Scrophularia striata for DPPH radical scavenging consistently decreases over the observation period. Conclusion: The data suggest that Scrophularia striata possesses antioxidant properties. The presence of flavonoids and phenolic compounds in Scrophularia striata highlights its potential to alleviate various disorders by modulating oxidative stress levels.

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Section: Frap Methodsmentioning

confidence: 99%

Evaluation of Antioxidant Potential and Free Radical Scavenging Activity of Methanol Extract from Scrophularia striata

Shiri,

Karimpour,

Sattari

et al. 2023

ABI

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“…Superoxide dismutase (SOD) and reduced glutathione (GSH) are enzymatic antioxidant defences. 6 Augmented myocardial oxidative stress and ROS generation, and deficit of antioxidant have been identified in MI. 7 , 8 The excessive ROS causes the peroxidation of lipid and protein, and DNA damage, which leads to cellular dysfunction and cell death.…”

Section: Introductionmentioning

confidence: 99%

“…In oxidative stress, the lipid peroxidation in membranes is usually evaluated by the formation of malondialdehyde (MDA). Superoxide dismutase (SOD) and reduced glutathione (GSH) are enzymatic antioxidant defences 6 . Augmented myocardial oxidative stress and ROS generation, and deficit of antioxidant have been identified in MI 7,8 .…”

Section: Introductionmentioning

confidence: 99%

HINT2 deficiency deteriorates oxidative stress in a mouse model of myocardial infarction

Huang

et al. 2023

ESC Heart Failure

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Aims Myocardial infarction (MI) is one of the serious diseases with great mortality over the world. Myocardial mitochondrial oxidative stress has been implicated as a key player in MI. The histidine triad nucleotide‐binding protein 2 (HINT2) is a nucleotide hydrolase and transferase located in mitochondria. HINT2 has multiple functions such as regulating mitochondrial lipid metabolism and respiration and glucose homeostasis. Although HINT2 has been shown to protect against MI, the underlying mechanisms were not fully elucidated. In this study, the effects of HINT2 on oxidative stress during MI were explored. Methods and results MI mouse models in both wild‐type and HINT2‐deficient mice were established. The expression of HINT2 in HINT2‐deficient mice was determined by quantitative real‐time PCR and western blot. The levels of oxidative stress were measured, including the levels of malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), and glutathione (GSH). The myocardial functions, as indicated by left ventricular end‐diastolic diameter (LVEDD), left ventricular end‐systolic diameter (LVESD), left ventricular ejection fraction (LVEF), and left ventricular fractional shortening (LVFS), were monitored. Both mRNA and protein expressions of HINT2 in the myocardial tissues were significantly down‐regulated in MI mice starting at 6 h post‐MI. MI induced oxidative stress 6 h post‐MI in myocardial tissues of wild‐type mice, as suggested by the enhanced MDA and NO levels and decreased SOD and GSH levels. The expression of HINT2 was negatively correlated to the MDA and NO levels and positively correlated to the SOD and GSH levels. HINT2‐deficient MI mice had significantly elevated levels of MDA and NO and significantly decreased levels of SOD and GSH when compared with wild‐type MI mice. HINT2‐deficient MI mice had higher LVEDD and LVESD and lower LVEF and LVFS compared with wild‐type MI mice, indicating that HINT2 deficiency exacerbated myocardial dysfunction. Conclusions HINT2 deficiency causes deteriorative oxidative stress in MI mice, leading to exacerbated myocardial dysfunction.

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“…The term OS refers to an imbalance between reactive oxygen species (ROS) formation and antioxidant defense systems. Under normal physiological circumstances, ROS levels are tightly modulated by enzymatic and non-enzymatic antioxidant defense systems [6,7]. In fact, OS can prompt the production and enhance the activity of pro-oxidant enzymatic systems that are known to be considerably implicated in the development of atherosclerosis [5].…”

Section: Introductionmentioning

confidence: 99%

The Association Between Arsenic Levels and Oxidative Stress in Myocardial Infarction: A Case–Control Study

et al. 2023

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BackgroundCardiovascular diseases (CVDs) are known as the rst causes of death throughout the world, and mainly myocardial infarction (MI), lead to 7.4 million deaths annually. Atherosclerosis is the major underlying cause of most CVDs. However, exposure to heavy metals, among other factors, deserves further attention as a risk factor for CVDs. This study was designed to evaluate the levels of arsenic (Ars) in myocardial infarction (MI) patients and healthy individuals as well as assess the association between the incidence of MI and Ars, total antioxidant capacity (TAC), and oxidative stress (OS). MethodsThis case-control study was conducted among patients with MI (n = 164) and normal individuals (n = 61) at Shafa Hospital in Kerman, Iran. Patients were classi ed into two groups, including coronary artery blocks above 50% (CAB > 50%, n = 83) and coronary artery blocks less than 50% (CAB < 50%, n = 83) based on their angiography ndings. The demographic characteristics, clinical history, biochemical parameters, and serum Ars and TAC levels were evaluated. ResultIn the present study, both CAB groups had signi cantly reduced levels of TAC compared with the control. Furthermore, TAC was lower in the CAB>%50 group compared to the CAB<%50 group. Ars levels were signi cantly higher in both CAB groups compared with the control. There was a signi cant positive relationship between CAB and Ars, BG, HbA 1 c, urea, creatinine, TG, TC, and LDL-c, as well as a negative relationship between HDL-c and TAC. Moreover, TAC levels showed a signi cant inverse correlation with Ars, HbA 1 c, and creatinine, and a positive correlation with HDL-c. As risk factors, Ars, hs-CRP, TG, TC, and LDL-c enhance the severity of the disease, and HDL-c and TAC decrease the disease severity. Moreover, ROC curve analysis revealed that the highest AUC for the CAB>%50 (AUC = 78.29), and cytotoxic levels for both CAB groups (Ars ≥ 0.105 ppm), and no signi cant differences were found between the two groups. ConclusionOur ndings suggest that Ars at ≥ 0.105 ppm is able to increase the risk of MI through the increased OS and decreased TAC.

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A comparative study on the effect of blood collection tubes on stress oxidative markers

Cited by 8 publications

References 33 publications

Evaluation of Antioxidant Potential and Free Radical Scavenging Activity of Methanol Extract from Scrophularia striata

Evaluation of Antioxidant Potential and Free Radical Scavenging Activity of Methanol Extract from Scrophularia striata

HINT2 deficiency deteriorates oxidative stress in a mouse model of myocardial infarction

The Association Between Arsenic Levels and Oxidative Stress in Myocardial Infarction: A Case–Control Study

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