A Comparative Study on the Effects of H2S and SO2Fumigation on the Growth and Accumulation of Sulphate and Sulphydryl Compounds inTrifolium pratenseL.,Glycine maxMerr. andPhaseolus vulgarisL.

Maas, F.M.; Kok, Luit J. De; Peters, Janny L.; Kuiper, P. J. C.

doi:10.1093/jxb/38.9.1459

Cited by 48 publications

(26 citation statements)

References 24 publications

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“…The soluble sulfur pool measured as sulfate, cysteine, γ ‐EC and GSH increased 1.5‐fold in WT leaves upon SO 2 exposure when compared with control plants grown under ambient air. This increase is mostly (approximately 98%) attributed to sulfate and is consistent with several other SO 2 exposure experiments (De Cormis 1969; Maas et al . 1987a; Kaiser, Dittrich & Heber 1993).…”

Section: Discussionsupporting

confidence: 92%

Sulfite oxidase controls sulfur metabolism under SO₂ exposure in Arabidopsis thaliana

Randewig

Hamisch

Herschbach

et al. 2011

Plant Cell & Environment

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In the present study, the significance of sulfite oxidase (SO) for sulfite detoxification and sulfur assimilation was investigated. In response to sulfur dioxide (SO2) exposure, a remarkable expansion of sulfate and a significant increase of GSH pool were observed in wild-type and SO-overexpressing Arabidopsis. These metabolic changes were connected with a negative feedback inhibition of adenosine 5Ј-phosphosulfate reductase (APR), but no alterations in gas exchange parameters or visible symptoms of injury. However, Arabidopsis SO-KO mutants were consistently negatively affected upon 600 nL L -1 SO2 exposure for 60 h and showed phenotypical symptoms of injury with small necrotic spots on the leaves. The mean g H O 2 ( ) was reduced by about 60% over the fumigation period, accompanied by a reduction of net CO2 assimilation and SO2 uptake of about 50 and 35%. Moreover, sulfur metabolism was completely distorted. Whereas sulfate pool was kept constant, thiol-levels strongly increased. This demonstrates that SO should be the only protagonist for back-oxidizing and detoxification of sulfite. Based on these results, it is suggested that co-regulation of SO and APR controls sulfate assimilation pathway and stabilizes sulfite distribution into organic sulfur compounds. In conclusion, a sulfate-sulfite cycle driven by APR and SO can be postulated for fine-tuning of sulfur distribution that is additionally used for sulfite detoxification, when plants are exposed to atmospheric SO2.

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Section: Discussionsupporting

confidence: 92%

Sulfite oxidase controls sulfur metabolism under SO₂ exposure in Arabidopsis thaliana

Randewig

Hamisch

Herschbach

et al. 2011

Plant Cell & Environment

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“…The absorbance at 415 nm was read 1 min after the addition of DTNB. The number of non-protein SH groups was established based on a curve prepared using l -cysteine and expressed as nanomoles –SH per gram fresh weight (Mass et al 1987). …”

Section: Methodsmentioning

confidence: 99%

Accumulation of heavy metals and antioxidant responses in Vicia faba plants grown on monometallic contaminated soil

Nadgórska–Socha

Kafel

Kandziora-Ciupa

et al. 2012

Environ Sci Pollut Res

107

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The purpose of this study was to explore the effects of soil contamination by selected metals (cadmium, copper, nickel, lead or zinc) on the antioxidant response of Vicia faba plants. The levels of the antioxidants: glutathione, proline, non-protein thiols, as well as guaiacol peroxidase and catalase activities were measured in the upperparts of plants. Additionally, the potential bioavailability of metals in the soil and their concentrations in V. faba plants were compared. Treatment with metal caused the problem of an elevation in its bioavailability in soil and its concentration in leaves and stems. The most serious problems seemed to be metal elevations in soil, especially Zn and Ni as well as in the aerial parts of V. faba plants. The antioxidant responses appeared to be metal specific. The elevation of guaiacol peroxidase activity in leaves and stems as well as the proline in leaves was the only more general reaction to metal exposure. Upon analysis of the effects of soil metal contamination on V. faba plants, we recommend the use of some measurements such as guaiacol peroxidase activity and proline level as useful tools in biological monitoring.

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“…1, Table II). We assume that the major nonprotein SH-compound measured in bean leaves is glutathione, because in shoots of several plant species glutathione comprises the major fraction of the total nonprotein SHcompounds, for example 95% in spruce needles (16), and more than 80% in red clover, soybean, and bean leaves (24). Glutathione is involved in detoxification of H202 and protection of SH-containing enzymes against oxidation, especially in chloroplasts ( 17).…”

Section: Activities Ofo2-and H202scavenging Enzymesmentioning

confidence: 99%

Magnesium Deficiency and High Light Intensity Enhance Activities of Superoxide Dismutase, Ascorbate Peroxidase, and Glutathione Reductase in Bean Leaves

Çakmak

Marschner²

1992

Plant Physiol.

1,538

749

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The influence of varied Mg supply (10-1000 micromolar) and light intensity (100-580 microeinsteins per square meter per second) on the concentrations of ascorbate (AsA) and nonprotein SH-compounds and the activities of superoxide dismutase (SOD; EC 1.15.11) and the H202 scavenging enzymes, AsA peroxidase (EC 1.11.1.7), dehydroascorbate reductase (EC 1.8.5.1), and glutathione reductase (EC 1.6.4.2) were studied in bean (Phaseolus vulgaris L.) leaves over a 13-day period. The concentrations of AsA and SH-compounds and the activities of SOD and H202 scavenging enzymes increased with light intensity, in particular in Mg-deficient leaves. Over the 12-day period of growth for a given light intensity, the concentrations of AsA and SH-compounds and the activities of these enzymes remained more or less constant in Mg-sufficient leaves. In contrast, in Mg-deficient leaves, a progressive increase was recorded, particularly in concentrations of AsA and activities of AsA peroxidase and glutathione reductase, whereas the activities of guaiacol peroxidase and catalase were only slightly enhanced. Partial shading of Mgdeficient leaf blades for 4 days prevented chlorosis, and the activities of the°2-and H202 scavenging enzymes remained at a low level. The results demonstrate the role of both light intensity and Mg nutritional status on the regulation of 02-and H202 scavenging enzymes in chloroplasts.Photoreduction of molecular 02 in chloroplasts is unavoidable and leads to the production of superoxide anion radicals (02 -) and H202 (1, 29). In nonstressed chloroplasts, photoreduction of 02 is estimated to be between 5 and 27% of the total electron flow (2, 3). Superoxide anion radicals, H202, and their derivatives hydroxyl radicals (OH,) and singlet oxygen ('02) are highly toxic, resulting in destructive effects on the functional and structural integrity of chloroplasts (8,17). As protection against these toxic 02 species, chloroplasts are equipped with several antioxidants and defense enzymes.SOD,2 mostly localized in chloroplasts in leaves (19)

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A Comparative Study on the Effects of H₂S and SO₂Fumigation on the Growth and Accumulation of Sulphate and Sulphydryl Compounds inTrifolium pratenseL.,Glycine maxMerr. andPhaseolus vulgarisL.

Cited by 48 publications

References 24 publications

Sulfite oxidase controls sulfur metabolism under SO₂ exposure in Arabidopsis thaliana

Sulfite oxidase controls sulfur metabolism under SO₂ exposure in Arabidopsis thaliana

Accumulation of heavy metals and antioxidant responses in Vicia faba plants grown on monometallic contaminated soil

Magnesium Deficiency and High Light Intensity Enhance Activities of Superoxide Dismutase, Ascorbate Peroxidase, and Glutathione Reductase in Bean Leaves

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A Comparative Study on the Effects of H2S and SO2Fumigation on the Growth and Accumulation of Sulphate and Sulphydryl Compounds inTrifolium pratenseL.,Glycine maxMerr. andPhaseolus vulgarisL.

Cited by 48 publications

References 24 publications

Sulfite oxidase controls sulfur metabolism under SO2 exposure in Arabidopsis thaliana

Sulfite oxidase controls sulfur metabolism under SO2 exposure in Arabidopsis thaliana

Accumulation of heavy metals and antioxidant responses in Vicia faba plants grown on monometallic contaminated soil

Magnesium Deficiency and High Light Intensity Enhance Activities of Superoxide Dismutase, Ascorbate Peroxidase, and Glutathione Reductase in Bean Leaves

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Product

Resources

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A Comparative Study on the Effects of H₂S and SO₂Fumigation on the Growth and Accumulation of Sulphate and Sulphydryl Compounds inTrifolium pratenseL.,Glycine maxMerr. andPhaseolus vulgarisL.

Sulfite oxidase controls sulfur metabolism under SO₂ exposure in Arabidopsis thaliana

Sulfite oxidase controls sulfur metabolism under SO₂ exposure in Arabidopsis thaliana