A comparative study on the leaf anatomical structure of Camellia oleifera in a low-hot valley area in Guizhou Province, China

Abstract

The leaf serves as an important assimilation organ of plants, and the anatomical structure of leaves can reflect the adaptability of the plant to the environment to a certain extent. The current study aimed to cultivate superior local cultivars, and 35 healthy individual plants were selected from the Camellia oleifera germplasm resource nursery for a comparative study of the leaf structure. In July 2019, the leaves were collected from 35 selected healthy C. oleifera plants, and the leaf structure was observed by using the paraffin section method. Healthy individual plants were screened using variance analysis, correlation analysis and cluster analysis. The representative indices were selected according to the cluster membership, correlation indices and coefficient of variation (C/V) for a comprehensive evaluation of drought resistance via the membership function. There were extremely significant differences in 11 indices of leaf structure for these 35 healthy plants. C18 had the greatest leaf thickness, C7 the largest spongy tissue, and C38 the largest ratio of palisade tissue thickness to spongy tissue thickness (P/S). The clustering results of the healthy individual plants differed significantly. The membership function showed that the drought resistance of 35 C. oleifera plants was divided into five categories. C18 had very strong drought resistance, and C3, C7 and C40 had strong drought resistance. There were significant differences in terms of the upper epidermis, P/S ratio and spongy tissue among the C. oleifera plants. C18, C3, C7 and C40 exhibited satisfactory drought resistance. Although C39 and C26 had moderate drought resistance, their P/S ratios were high, which might be used to cultivate high-yield and drought-resistant C. oleifera varieties. The leaf P/S ratio of C. oleifera from low-hot valley areas was high. Among various leaf structures, spongy tissue, upper epidermis, P/S ratio and cuticle constitute the drought resistance evaluation indices for C. oleifera grown in low-hot valley areas.

Cytological, physiological and transcriptomic analysis of variegated Leaves in Primulina pungentisepala offspring

“…The samples were cut into paraffin Sect. (8 μm) with a microtome [ 42 ]. Other samples were fixed in 2.5% glutaraldehyde overnight at 4 °C.…”

Genotypic and Phenotypic Diversity of Endemic Golden Camellias Collected from the North of Vietnam

“…The leaves structures and characteristics are an important criterion for taxonomy identification. [34] Recently, the leaf morphology and anatomy of 60 species of the Camellia genus were described, in which leaf thickness and the area of the midrib showed significant diversity. [35] In this study, we enlarge the number of examined parameters related to the anatomical construction of the midvein (midvein width, depression angle, number of collenchyma cell layer, phloem area, bundle sheath area, xylem area) and blade (palisade/spongy ratio) to validate the above-mentioned genetic discrimination.…”

“…The number of palisade cell layers as well as the palisade/spongy ratio was specific for each species; moreover, these parameters were strongly affected by the rainfall amount. [34] In our study, C. euphlebia was gathered in Hai Ha -Quang Ninh where the annual rainfall is much higher while the others were collected from Tam Dao -Vinh Phuc province. [36] This geographical characteristic contributes to the great discrimination in the palisade/spongy ratio of C. euphlebia compared to other species.…”

Morphological, physicochemical, and phytochemical characterization of Camellia dormoyana (Pierre) Sealy from Vietnam

“…Meanwhile, palisade tissue with 2 layers, such as C. sinensis and C. assamica subsp. lasiocalyx (Rajanna and Ramakrishnan 2010;Ekayanti et al 2017) and 3-4 layers, such as C. oleifera (Hu et al 2022). Thus, it is possible to rely on the number of layers of palisade tissue to classify Camellia species together.…”

“…Similar to other genera of Theaceae, trichomes on surfaces of C. dormoyana midrib were unicellular nongland. Trichomes were found in both blade and midrib leaves C. sinensis, C. oleifera, C. phellocapsa, C. jinshajiangica, C. villosa, etc (Erxu et al 2009;Ekayanti et al 2017;Hu et al 2022), while C. dormoyana was only found on the upper midrib surface. According to the Vietnamese Pharmacopoeia V's guidelines for quality control of medicinal herbs (Ministry of Health 2017), the type of leaf epidermal stomata is usually a relatively constant feature for a species.…”