A comparative study on the blood and milk cell counts of healthy, subclinical, and clinical mastitis Karan Fries cows

Abstract

Aim:The present study was aimed to study the use of cell counts as an early indicator of mammary health.Materials and Methods:Milk and blood cell counts were estimated from 8 healthy, 8 subclinical (SCM), and 8 clinically mastitis (CM) groups of Karan Fries (KF) cows.Results:Total leucocyte counts and neutrophil percent in blood and milk somatic cells and milk neutrophil percent of healthy cows increased significantly (p<0.05) in SCM cows and CM cows. Viability of blood and milk neutrophils was more in healthy cows, but decreased significantly (p<0.05) in SCM and CM cows. Significant (p<0.05) decrease were also observed in both the blood and milk lymphocytes and monocytes of SCM and CM cows. Phagocytic activity (PA) of blood neutrophils also decreased significantly (p<0.05) in SCM cows. There was no difference between the PA of SCM and CM cows. Milk neutrophil percent was more in the SCM and clinically infected milk than in the blood of these cows. About 96-97% of the neutrophils had segmented nucleus in both healthy and subclinical milk, whereas, 2-3% were having band shaped or immature nuclei. There was a significant decrease in the segmented neutrophils, whereas, band neutrophils increase significantly to about 5% in the infected milk of mastitic cows. Viability of the milk neutrophils decreased more in case of subclinical and clinical milk as compared to that of blood. PA was found to be highest in the milk of healthy group of cows, but decreased significantly (p<0.05) in subclinically infected cows. However, there was no difference between the PA of milk neutrophils of SCM and CM cows. PA of milk was also found to be significantly lower in the milk of healthy cows when compared to that of blood neutrophils.Conclusion:This study indicated that percent neutrophils and their type in conjunction with milk somatic cell counts can be used as a more reliable indicator of mammary health in cows.

A survey on the incidence of Prototheca mastitis in dairy herds in Lublin province, Poland

“…Interestingly, no significant changes in blood cell counts were found between mastitis and control cows or between SCM and CM animals. This is in contrast with what was observed by Alhussien et al (2015), where total leukocyte counts of CM cows were higher than those in SCM cows.…”

Safety and efficacy of a mesenchymal stem cell intramammary therapy in dairy cows with experimentally induced Staphylococcus aureus clinical mastitis

“…Moreover, presence of flakes in milk with no swelling of the gland or systemic illness indicate that experimental inoculation of S. aureus resulted in mild clinical mastitis. This diminished inflammatory reaction, possible associated to reduced immune response, corresponds to the in vivo model recently described for induced www.nature.com/scientificreports www.nature.com/scientificreports/ mastitis after S aureus udder infection 37 . Among infected quarters, treatment with AT-MSCs resulted in similar SCC compared to treatment with antibiotics or vehicle.…”

Amount of Milk Neutrophil Percentage and Associated CD Molecular Changes on the Compositional and Technological Properties of Milk

“…It occurs due to migration of more immature neutrophils from the bone marrow to the mammary gland to fight infection [1]. However, due to immaturity in their development, they cannot exhibit their full potential.…”

“…The cows were then divided into 3 groups viz. ; healthy (n=6), subclinical (n=6) and clinical mastitis (n=6) according to their SCC and California mastitis test in them [1]. The cows were managed as per the practices followed in the institute's herd and were offered ad lib green fodder and a calculated amount of concentrate mixture.…”