A comparative study on the efficacy of CD8-positive cells in enhancing allogeneic bone marrow engraftment: cell sorting vs microbead selection

Abstract

Summary:We have used a superparamagnetic microbead selection system to positively select a murine bone marrow CD8 + cell population. The functional ability of these cells to enhance allogeneic bone marrow engraftment was compared with that of fluorescence activated cell sorter purified CD8 ؉ cells. The CD8 ؉ cell population prepared by the microbead selection procedure was as effective as cell sorter purified CD8 ؉ cells in enhancing T celldepleted allogeneic bone marrow engraftment in lethally irradiated mice. Phenotypic characterization of these cells shows that most of these CD8 ؉ cells express CD3 and the T cell antigen receptor complex.

Engraftment and survival following reduced-intensity allogeneic peripheral blood hematopoietic cell transplantation is affected by CD8+ T-cell dose

“…In a myeloablative murine model of transplantation, both CD4 ϩ and CD8 ϩ cells from the donor can cause GVHD, but donor CD8 ϩ cells were more than 5-fold more effective than CD4 ϩ cells for preventing graft rejection mediated by host immune cells remaining after myleoablation. 20,21 Similarly, other experimental models have shown that donor-derived bone marrow-resident CD8 ϩ cells, but not CD4 ϩ cells, directly facilitate engraftment of purified hematopoietic stem cells. 22,23 In humans, CD4 ϩ cell removal from donor bone marrow does not hinder engraftment, whereas a correlation between graft failure and depletion of donor CD8 ϩ cells has been reported following myeloablative AHCT.…”