A Comparative Study on Polyphenolic Composition of Berries from the Tibetan Plateau by UPLC‐Q‐Orbitrap MS System

Abstract

Five traditional medicinal food from the Tibetan plateau including Nitraria tangutorum Bobrov (NT), Hippophae rhamnoides L. (HR), Lycium ruthenicum Murray (LR), Lycium barbarum L. (LB) and Rubus corchorifolius L.f. (RC) are rich in phenolic compounds. However, the detailed studies about the phenolic compounds remain scarce. Therefore, we established a rapid method for the simultaneous identification and quantification of the phenolic compounds from berries via Ultra Performance Liquid Chromatography-Quadruple-Orbitrap MS system (UPLC-Q-Orbitrap MS). This method was verified from many aspects including detection limit, quantification limit, precision, repeatability, stability, average recovery rate and recovery range, and then was used to analyze the phenolic compounds in these five species of berries. Finally, a total of 21 phenolic compounds were directly identified by comparing the retention time and exact mass, of which 14 compounds were identified by us for the first time in berries from the Tibetan plateau, including one flavonoid aglycone (myricetin), 11 phenolic acids (gallic acid, protocatechuate, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, 2-hydroxybenzeneacetic acid and ellagic acid), one flavanol (catechin) and one dihydrochalcone flavonoid (phloretin). Quantitative results showed that rutin, myricetin, quercetin and kaempferol were the main flavonoids. Moreover, a variety of phenolic acid compounds were also detected in most of the berries from the Tibetan plateau. Among these compounds, the contents of protocatechuate and chlorogenic acid were high, and high levels of catechin and phloretin were also detected in these plateau berries.

Breeding Buckwheat for Nutritional Quality in the Czech Republic

“…Identification of phenolic compounds in buckwheat samples ( Figure S1 ) was based on their retention times relative to the authentic standards and on mass spectral data (accurate mass determination generating elemental composition and fragmentation patterns of a molecular ion) obtained by LC-MS, which were compared with those described in previous studies made on Orbitrap analysis of phenolic compounds [ 20 , 22 , 64 ].…”

Kaempferol and Kaempferide Attenuate Oleic Acid-Induced Lipid Accumulation and Oxidative Stress in HepG2 Cells

“…Kaempferol and kaempferide were isolated from Hippophae rhamnoides L., as previously described [20,56]. OA, oil red O and sulforhodamine B (SRB) were purchased from Sigma-Aldrich (St. Louis, MO, USA).…”

Antiproliferative and Pro-Apoptotic Effects of a Phenolic-Rich Extract from Lycium barbarum Fruits on Human Papillomavirus (HPV) 16-Positive Head Cancer Cell Lines

“…Figure 1 presents the total ion chromatogram (TIC). Compounds were identified based on detailed scrutiny of their mass spectra using the precursor ion, fragment ions, and comparison of the fragmentation patterns with molecules described in the literature [ 23 , 25 , 26 , 27 ]. Table 1 summarizes the identification of these compounds, and they are numbered according to their retention times.…”

“…Total ion chromatograms (TIC) were processed in MzMine 2 software (Whitehead Institute for Biomedical Research, Cambridge, MA, USA). Compounds were annotated after detailed scrutiny of the ultraviolet-visible and high-resolution mass spectral (exact mass of quasi-molecular and fragment ions) data of each signal, compared to the information previously reported in the literature for Lycium species [ 25 , 26 , 27 , 30 ].…”