A comparative study on effect of platelet rich plasma Vs conventional dressing in healing rate of chronic diabetic ulcers

Abstract

Introduction and Aim: This study has been undertaken to evaluate efficacy of platelet rich plasma (PRP) dressing that is recently introduced with conventional dressing. We have tried to compare PRP dressing with conventional normal dressing in healing the wound. Materials and Methods: We conducted this study in 120 patients of chronic non healing diabetic foot ulcers who were treated over the period of three years. Number of cases (60 each) were studied for efficacy of applying autologous platelet rich plasma versus normal dressing for chronic non healing diabetic foot ulcers. At every week follow up, the ulcer was examined for granulation tissue, area and volume which was calculated using Vernier calipers and marked on a graph paper and photographs were taken. Wound healing was compared between PRP and normal dressing. Results: The incidence of male susceptibility was more (70%) than females (30%) in diabetic foot. Average rate of healing per week was found to be significantly better among the PRP group than normal dressing group. The ulcer showed significant reduction in size among the PRP group in comparison to the normal dressing group with a significant P value (0.001). Conclusion: In this study application of platelet rich plasma into chronic non-healing diabetic foot ulcers in comparison with conventional dressing has shown a significant reduction in the size of the ulcer, hospital stay and cost for the patient.

Autologous blood products: Leucocyte and Platelets Rich Fibrin (L-PRF) and Platelets Rich Plasma (PRP) gel to promote cutaneous ulcer healing – a systematic review

“…Only two studies, Goda20 and Somani and Rai21, had L-PRF gel as an intervention, the remaining five had PRP gel. Among the seven studies, two were conducted in India,21 22 two in Egypt,20 23 and the remaining studies were conducted in the USA,24 China25 and Turkey,26 respectively. Four studies were conducted in patients with diabetic ulcers,22–25 two in patients with venous leg ulcers20 21 and one in patients with pressure ulcers 26…”

“…Only five studies reported the proportion of ulcers completely healed, four studies reported time to complete healing and five studies reported the rate of healing per unit of time. Five studies reported mean age in years at baseline in terms of mean and SD21–24 26 The mean age (SD) ranged from 39.3 (8.2) to 68.3 (6.4) years. The sample size ranged between 9 and 60 subjects in both intervention and control groups.…”

“…Within the seven included studies, there is a variability in the method of ulcer measurements. Rajendran et al measured the ulcer area using the measurement (length and breadth) taken with the help of vernier callipers and marked on graph paper 22. Li et al 25 calculated the area using the picture processing software ImageJ V.1.46h (National Institute of Health, Bethesda, Maryland, USA) 27.…”

“…Rajendran et al followed the double spin method. In the first spin, 20 mL of venous blood was collected in a tube containing acid dextrose solution and then centrifuged at 5000 rpm for 15 min 22. This first spin was to separate the supernatant and buffy coat from the red blood cells.…”

“…Online supplemental figure 1 (traffic light plot) demonstrates the risk of bias for the individual domains of each study. Six studies20–24 26 had an overall high risk of bias with only one study showing a low risk of bias. For domain 1 on bias arising from the randomisation process, two studies21 22 showed a high risk of bias and two showed some concerns.…”