A comparative study of type A and type B gelatin nanoparticles as the controlled release carriers for different model compounds

Aramwit, Pornanong; Jaichawa, Nungruthai; Ratanavaraporn, Juthamas; Srichana, Teerapol

doi:10.1166/mex.2015.1233

Cited by 59 publications

(45 citation statements)

References 24 publications

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“…Amounts of lysine and hydroxylysine residues in different types of gelatins have been reported in the literature . Based on those contents and molar balance calculations, approximately 0.34 mmol primary amine groups g −1 gelatin A and 0.37 mmol primary amine groups g −1 gelatin B were assumed, which are in good agreement with reported values in the literature …”

Section: Methodssupporting

confidence: 87%

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Self‐Healing, Injectable Gelatin Hydrogels Cross‐Linked by Dynamic Schiff Base Linkages Support Cell Adhesion and Sustained Release of Antibacterial Drugs

Vahedi

Barzin

Shokrolahi

et al. 2018

Macro Materials & Eng

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A new class of dynamic hydrogels made through Schiff base bonds based on gelatin (type A and B) and polyethylene glycol dibenzaldehyde (diBA‐PEG, 2000 and 4000 g mol−1) is developed. Hydrogels form in situ by mixing aqueous solutions of gelatin and diBA‐PEG at a carefully adjusted pH. Compression test shows that the samples based on gelatin A are able to withstand at least ten cyclic loading/unloading without crack formation and significant permanent deformation. Self‐healing behavior of the hydrogel is proved by rheological measurements and also visual method. This hydrogel is proven to be injectable and nontoxic. Performance of the hydrogel in loading and delivery of clindamycin hydrochloride, as an antibacterial model drug, is evaluated against Staphylococcus aureus via antibacterial activity test. In vitro release of clindamycin hydrochloride is studied through an innovative method and it becomes clear that the release of clindamycin hydrochloride from this hydrogel follows a zero‐order kinetics.

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Section: Methodssupporting

confidence: 87%

“…[22] Based on those contents and molar balance calculations, approximately 0.34 mmol primary amine groups g −1 gelatin A and 0.37 mmol primary amine groups g −1 gelatin B were assumed, which are in good agreement with reported values in the literature. [23][24][25][26]…”

Section: Hydrogel Preparationmentioning

confidence: 99%

Self‐Healing, Injectable Gelatin Hydrogels Cross‐Linked by Dynamic Schiff Base Linkages Support Cell Adhesion and Sustained Release of Antibacterial Drugs

Vahedi

Barzin

Shokrolahi

et al. 2018

Macro Materials & Eng

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“…This phenomenon could be explained as follows: first, acidic‐derived type A gelatin has an isoelectric point (IEP) of 8–9, thus a positive charge at physiologic pH, however, the growth factors derived from PRP with an IEP above 8.5 are also positively charged. Although some research has demonstrated that like charges can still be put together, the molecular weight of the biomolecules and size of the carriers also influence the release behavior and the electrostatic interaction . The repulsive force of like charges may weaken the efficiency of the growth factors attachment on the microspheres.…”

Section: Resultsmentioning

confidence: 95%

Injectable Mussel‐Inspired Immobilization of Platelet‐Rich Plasma on Microspheres Bridging Adipose Micro‐Tissues to Improve Autologous Fat Transplantation by Controlling Release of PDGF and VEGF, Angiogenesis, Stem Cell Migration

Zhou

Chang

et al. 2017

Adv Healthcare Materials

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Platelets-rich plasma (PRP) can produce growth factors (GFs) to improve angiogenesis. However, direct injection of PRP does not lead to highly localized GFs. The current study employs a mussel-inspired polydopamine to immobilize PRP on gelatin microspheres (GMs) with the purpose of bridging adipose micro-tissues to help implanted fat survive (GM-pDA-PRP). Enhanced PRP adhesion leads to a prolonged and localized production of GFs, which is verified by platelet counting and by ELISA of vascular endothelial growth factors (VEGFs) and of platelet derived growth factors (PDGFs). The GM-pDA-PRP "hatches" a microenvironment for the proliferation of adipose-derived stem cells. After the adipose micro-tissue has bridged with GM-pDA-PRP after 16 weeks, triple-fluorescence staining reveals that the mature adipocytes, blood vessels, and capillaries are arranged like in normal adipose tissue. The survival fat increases significantly compared to that in control, PRP, and GM-PRP groups (84.8 ± 11.4% versus 47.8 ± 8.9%, 56.9 ± 9.7%, and 60.2 ± 10.5%, respectively). Both histological assessments and CD31 immunofluorescence indicate that the improvement of angiogenesis in GM-pDA-PRP is higher than in the fat graft group (6.4-fold in quantitative CD31 positive cells). The CD34 positive cells in the GM-pDA-PRP group are around 3.5-fold the amount in the fat graft group, which suggests that more stem cells migrate to the implant area. Cell proliferation staining shows that the number of Ki67 positive cells is around five times as high as that in the fat graft group.

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“…For example, gelatin type A has a higher gel strength and glycine and proline contents. The pI for type A is between 8 and 9, exhibiting a positive charge at neutral pH; whereas, type B has a pI value between 4.8 and 5.4, bearing negative charge at neutral pH …”

Section: Characteristics Of Gelatin and Polysaccharides As Natural Bimentioning

confidence: 99%

“…The pI for type A is between 8 and 9, exhibiting a positive charge at neutral pH; whereas, type B has a pI value between 4.8 and 5.4, bearing negative charge at neutral pH. 52,53 Polysaccharides may be sourced from crabs, lobsters, shrimps, forests (biomass), and bacteria (Figure 2a). 54 In this review, polysaccharides such as cellulose, chitin, chitosan, alginate, and HA are highlighted and their synergy with gelatin in 3D cellular engineering is presented.…”

Section: Characteristics Of Gelatin and Polysaccharides As Natural mentioning

confidence: 99%

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Afewerki

Sheikhi

Kannan

et al. 2018

Bioengineering & Transla Med

290

210

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Gelatin is a promising material as scaffold with therapeutic and regenerative characteristics due to its chemical similarities to the extracellular matrix (ECM) in the native tissues, biocompatibility, biodegradability, low antigenicity, cost‐effectiveness, abundance, and accessible functional groups that allow facile chemical modifications with other biomaterials or biomolecules. Despite the advantages of gelatin, poor mechanical properties, sensitivity to enzymatic degradation, high viscosity, and reduced solubility in concentrated aqueous media have limited its applications and encouraged the development of gelatin‐based composite hydrogels. The drawbacks of gelatin may be surmounted by synergistically combining it with a wide range of polysaccharides. The addition of polysaccharides to gelatin is advantageous in mimicking the ECM, which largely contains proteoglycans or glycoproteins. Moreover, gelatin–polysaccharide biomaterials benefit from mechanical resilience, high stability, low thermal expansion, improved hydrophilicity, biocompatibility, antimicrobial and anti‐inflammatory properties, and wound healing potential. Here, we discuss how combining gelatin and polysaccharides provides a promising approach for developing superior therapeutic biomaterials. We review gelatin–polysaccharides scaffolds and their applications in cell culture and tissue engineering, providing an outlook for the future of this family of biomaterials as advanced natural therapeutics.

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A comparative study of type A and type B gelatin nanoparticles as the controlled release carriers for different model compounds

Cited by 59 publications

References 24 publications

Self‐Healing, Injectable Gelatin Hydrogels Cross‐Linked by Dynamic Schiff Base Linkages Support Cell Adhesion and Sustained Release of Antibacterial Drugs

Self‐Healing, Injectable Gelatin Hydrogels Cross‐Linked by Dynamic Schiff Base Linkages Support Cell Adhesion and Sustained Release of Antibacterial Drugs

Injectable Mussel‐Inspired Immobilization of Platelet‐Rich Plasma on Microspheres Bridging Adipose Micro‐Tissues to Improve Autologous Fat Transplantation by Controlling Release of PDGF and VEGF, Angiogenesis, Stem Cell Migration

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

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