A comparative study of two chemical models for creating subsurface caries lesions on aprismatic and prismatic enamel

Abstract

Purpose: To investigate the mineral density and lesion depth of artificial caries lesions on aprismatic enamel and prismatic enamel created by lactic acid and acetic acid buffers. Methods: Forty bovine enamel blocks were allocated to: aprismatic enamel (Group A) and prismatic enamel (Group C) in acetic acid buffer for 192 h and aprismatic enamel (Group B) and prismatic enamel (Group D) in lactic acid buffer for 96 h. The mineral loss and lesion depth were measured using micro-computed tomography. Results: A significant difference (P = 0.01) was observed in the mineral loss (%) in the lesions on aprismatic enamel and prismatic enamel treated with lactic acid buffer while no significant difference (P = 0.51) was observed in the mineral loss (%) in the lesions on aprismatic enamel and prismatic enamel treated with acetic acid buffer. No significant difference was noted in the mean lesion depth of lesions on aprismatic enamel and prismatic enamel treated with acetic acid and lactic acid buffers (P > 0.05). Conclusion: Aprismatic enamel and prismatic enamel have similar mineral loss in acetic acid while prismatic enamel showed more mineral loss compared to aprismatic enamel in lactic acid.

Combined Effect of a Bioinspired Self-Assembling Peptide and Fluoride Varnish on Remineralisation of Artificial Early Enamel Caries Lesion: An in vitro Study

“…Twelve specimens without nail polish were prepared for X-ray diffraction (XRD) analysis. All the specimens were then immersed in demineralisation solution (0.1 m lactic acid, 0.16 m m calcium phosphate in distilled water, and pH adjusted to 4.4 with 5 m KOH) for 96 h at 37°C with solutions refreshed daily to create an approximately 200 μm early enamel caries lesion [18]. Subsequent to the formation of early enamel caries lesion, a double layer of acid-resistant nail polish was applied on the second zone which served as another internal control of the early enamel caries lesion.…”

“…Approximately 200 μm of enamel was consistently polished off from the specimens' surface with 1,200 SiC paper (Struers, Copenhagen, Denmark) on a TegraPol-21 polisher (Struers, Copenhagen, Denmark) to remove the aprismatic layer [17,18]. Three indents with approximately 1 mm in depth were sliced into the specimens to divide the surface into three zones using a diamond disc.…”

The subsurface lesion in erosive tooth wear

“…Furthermore, Wong et al. demonstrated how the outermost layer of enamel, referred to as the aprismatic layer, played a significant role in the resistance against dental caries due to its strong resistance against acid dissolution [9] . One of the hypothesised reasons for this is the aprismatic region's higher mineral content in the outer 10–30 µm of the enamel [ 10 , 11 ].…”