A Comparative Study of the Effectiveness of Diagnostic Tests for Visceral Leishmaniasis

Boelaert, Marleen; Rijal, Suman; Regmi, Shyam Raj; Singh, Rupa; Karki, B. M. S.; Jacquet, D.; Chappuis, François; Campino, Lenea; Desjeux, Philippe; Ray, D. Le; Koirala, S; Stuyft, Patrick Van der

doi:10.4269/ajtmh.2004.70.72

Cited by 144 publications

(144 citation statements)

References 19 publications

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“…Additionally, the same location, the estimated direct cost of treatment with amphotericin B deoxycholate was USD 260.00 25 . Boelaert et al 26 estimated a direct cost of USD 150.00 for Glucantime ® treatment in Sudan. Vanlerberghe et al 27 reported that the estimated direct costs of diagnosis using a rapid test and treatment with N-methylglucamine antimoniate, amphotericin B, and liposomal amphotericin B deoxycholate in various endemic areas were USD 120.10, USD 111.10, and USD 537.50, respectively.…”

Section: Discussionmentioning

confidence: 99%

The direct costs of treating human visceral leishmaniasis in Brazil

Assis

Rosa

Teixeira

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction:The drugs available for visceral leishmaniasis (VL) treatment in Brazil have specific characteristics in terms of operability, effectiveness, toxicity, and cost. The aim of this study was to estimate the direct costs of therapies recommended by the Ministry of Health (MH) for VL treatment in Brazil. Methods: The analytical perspective used was that adopted by the Brazilian Public Health System. Three drugs and four regimens were included: 1) N-methyl glucamine antimoniate intramuscularly at 20mg per kg per day for 30 days; 2) N-methyl glucamine antimoniate intravenously at 20mg per kg per day for 30 days; 3) amphotericin B deoxycholate at 1mg per kg per day for 21 days; and 4) liposomal amphotericin B at 3mg per kg per day for a 7 days treatment. Results: The estimated direct costs of treatment for an adult patient using N-methylglucamine antimoniate administered via the intramuscular and intravenous routes were USD 418.52 and USD 669.40, respectively. The estimated cost of treatment with amphotericin B deoxycholate was USD 1,522.70. Finally, the estimated costs of treatment with liposomal amphotericin B were USD 659.79, and USD 11,559.15 using the price adopted by the WHO and the Drug Regulation Board, respectively. Conclusions: This analysis indicates the economic feasibility of replacing N-methyl glucamine antimoniate with liposomal amphotericin B, which allows a shorter treatment period with less toxicity compared with other treatments, provided that the purchase value used by the WHO and transferred to the MH is maintained.

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Section: Discussionmentioning

confidence: 99%

The direct costs of treating human visceral leishmaniasis in Brazil

Assis

Rosa

Teixeira

et al. 2017

Rev. Soc. Bras. Med. Trop.

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“…7 For CL, microscopy of skin smears and culturing parasites are associated with low sensitivity. 6 , 8 For VL a range of serological diagnostic techniques have been developed that are both sensitive and cost effective; the direct agglutination test (DAT) and the rK39 tests are extensively used in Asia and Africa 9 ; rK39 is also the diagnostic of choice for kala-azar elimination from South-East Asia. 10 , 11 However, antibodies detected by these tests can be found in the blood of infected patients for many months after the parasites have disappeared, making detection of active infection inaccurate; moreover, serological tests may not be used on immuno-compromised individuals.…”

Section: Introductionmentioning

confidence: 99%

Development of a Reverse Transcriptase Loop-Mediated Isothermal Amplification (LAMP) Assay for the Sensitive Detection of Leishmania Parasites in Clinical Samples

Adams¹,

Schoone²,

Ageed³

et al. 2010

The American Journal of Tropical Medicine and Hygiene

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Abstract. Here we describe a generic, reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay, for the identification of Leishmania species from clinical samples. LAMP is an isothermal reaction recently developed as a point-of-care diagnostic tool. Primers were designed in the conserved region of the 18S ribosomal RNA (rRNA) gene; amplification was visualized by the pre-amplification addition of fluorescent detection reagent (FDR) and a simple UV lamp. By using a reverse-transcriptase step, the system detected infections between 10 and 100 parasites per mL. The assay was tested on a range of nucleic acid extracts from Leishmania species, visceral leishmaniasis (VL) patients from Sudan, and cutaneous leishmaniasis (CL) patients from Suriname. The sensitivity of RT-LAMP from the blood of VL patients was 83% ( N = 30) compared with microscopy of bone-marrow and lymph-node aspirates; for CL patients the observed sensitivity was 98% ( N = 43). The potential to use LAMP as a diagnostic tool for leishmaniasis is discussed.

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“…This infection has several diverse clinical manifestations: cutaneous, mucosal and most notably visceral leishmaniasis, and is caused by Leishmania amazonensis, Leishmania braziliensis, and Leishmania donovani species. [1][2][3][4] An estimated 1.5 to 2 million new cases of leishmaniasis occurs each year in the world, which in the visceral manifestation is often fatal if untreated. [5][6][7][8][9] Unfortunately non-availability of satisfactory chemotherapeutic agents and failure to develop an effective Synthesis, X-ray Crystal Structure and Theoretical Calculations J. Braz.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, X-ray crystal structure and theoretical calculations of antileishmanial neolignan analogues

Nascimento¹,

Santos²,

Santos³

et al. 2010

J. Braz. Chem. Soc.

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A síntese e a estrutura cristalina por difração de raios-X de dois análogos de neolignanas, 2-(4-clorofenil)-1-feniletanona (20) e 2-[tio(4-clorofenil)]-1-(3,4-dimetoxifenil)propan-1-ona (12) são descritas. O composto 12 apresenta atividade intracelular contra Leishmania donovani e Leishmania amazonensis de amastigotas que causam a leishmaniose tegumentar e visceral. Além disso, a teoria do funcional de densidade (DFT) com o funcional híbrido B3LYP foi empregado para calcular um conjunto de descritores moleculares para dezenove análogos sintéticos de neolignanas com atividades antileishmaniose. Posteriormente, a análise discriminante stepwise foi realizada para investigar possíveis relações entre a estrutura molecular e atividades biológicas. Por meio dessa análise os compostos foram classificados em dois grupos ativos e inativos de acordo com seu grau de atividade biológica, e as propriedades mais importantes foram as cargas de alguns átomos, a afinidade eletrônica e o ClogP.The synthesis and X-ray crystal diffraction structure of two analogues of neolignans, 2-(4-chlorophenyl)-1-phenylethanone (20) and 2-[(4-chlorophenyl)thio]-1-(3,4-dimethoxyphenyl) propan-1-one (12) is described. The compound 12 presents activity against intracellular Leishmania donovani and Leishmania amazonensis amastigotes that cause cutaneous and visceral leishmaniasis. In addition, the density functional theory (DFT) with the B3LYP hybrid functional was employed to calculate a set of molecular descriptors for nineteen synthetic analogues of neolignans with antileishmanial activities. Afterwards, the stepwise discriminant analysis was performed to investigate possible relationship between the molecular descriptors and biological activities. Through this analysis the compounds were classified into two groups active and inactive according to their degree of biological activities, and the more important properties were charges on some key atoms, electronic affinity and ClogP.

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A Comparative Study of the Effectiveness of Diagnostic Tests for Visceral Leishmaniasis

Cited by 144 publications

References 19 publications

The direct costs of treating human visceral leishmaniasis in Brazil

The direct costs of treating human visceral leishmaniasis in Brazil

Development of a Reverse Transcriptase Loop-Mediated Isothermal Amplification (LAMP) Assay for the Sensitive Detection of Leishmania Parasites in Clinical Samples

Synthesis, X-ray crystal structure and theoretical calculations of antileishmanial neolignan analogues

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