A comparative study of the BBL crystal enteric/nonfermenter identification system and the biomerieux API20E and API20NE identification systems after overnight incubation

Varettas, Kerry; Mukerjee, Chinmoy; Schmidt, Michael

doi:10.1080/00313029500169303

Cited by 10 publications

(6 citation statements)

References 9 publications

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“…The limited number of S. maltophilia strains used in many of these evaluations (e.g., the study of Varettas et al, in which a single strain was used [426]), however, precludes definitive conclusions about the relative abilities of each system with respect to correct identification of this particular organism. Where comparative studies have evaluated automated and nonautomated systems, there have been no significant differences in their ability to identify S. maltophilia (304,356).…”

Section: Identification In the Clinical Laboratorymentioning

confidence: 99%

Microbiological and Clinical Aspects of Infection Associated with Stenotrophomonas maltophilia

1998

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SUMMARY The gram-negative bacterium Stenotrophomonas maltophilia is increasingly recognized as an important cause of nosocomial infection. Infection occurs principally, but not exclusively, in debilitated and immunosuppressed individuals. Management of S. maltophilia-associated infection is problematic because many strains of the bacterium manifest resistance to multiple antibiotics. These difficulties are compounded by methodological problems in in vitro susceptibility testing for which there are, as yet, no formal guidelines. Despite its acknowledged importance as a nosocomial pathogen, little is known of the epidemiology of S. maltophilia, and although it is considered an environmental bacterium, its sources and reservoirs are often not readily apparent. Molecular typing systems may contribute to our knowledge of the epidemiology of S. maltophilia infection, thus allowing the development of strategies to interrupt the transmission of the bacterium in the hospital setting. Even less is known of pathogenic mechanisms and putative virulence factors involved in the natural history of S. maltophilia infection and this, coupled with difficulties in distinguishing colonization from true infection, has fostered the view that the bacterium is essentially nonpathogenic. This article aims to review the current taxonomic status of S. maltophilia, and it discusses the laboratory identification of the bacterium. The epidemiology of the organism is considered with particular reference to nosocomial outbreaks, several of which have been investigated by molecular typing techniques. Risk factors for acquisition of the bacterium are also reviewed, and the ever-expanding spectrum of clinical syndromes associated with S. maltophilia is surveyed. Antimicrobial resistance mechanisms, pitfalls in in vitro susceptibility testing, and therapy of S. maltophilia infections are also discussed.

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Section: Identification In the Clinical Laboratorymentioning

confidence: 99%

Microbiological and Clinical Aspects of Infection Associated with Stenotrophomonas maltophilia

1998

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“…Recently, automated systems have been developed to identify gram-negative bacteria (24,29,33,34,45), and these have often been unable to accurately identify aeromonads to the species level or as part of a Aeromonas complex (Aeromonas hydrophila group); however, the reports about the evaluation of these systems do not include information about a high number of Aeromonas species.…”

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confidence: 99%

Identification of Motile Aeromonas Strains with the MicroScan WalkAway System in Conjunction with the Combo Negative Type 1S Panels

Vivas

Saa

Tinajas³

et al. 2000

Appl Environ Microbiol

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“…Other automatic methods started to appear in this decade including improvements of the API 20E system (for a more detailed review see [39]), in an attempt to reduce the time needed for the procedure using recurring automations. The BBL Crystal (Becton Dickinson, NJ, USA) [45] is also a variation of the API system. An automated version of the API is the Vitek ® system (bioMérieux, Craponne, France), first developed in the 1970s, which eliminated the subjectivity of the reading of test reactions [46].…”

Section: Biochemical Analytical Methods To Detect Microorganismsmentioning

confidence: 99%

Advances in Chemical and Biological Methods to Identify Microorganisms—From Past to Present

et al. 2019

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Fast detection and identification of microorganisms is a challenging and significant feature from industry to medicine. Standard approaches are known to be very time-consuming and labor-intensive (e.g., culture media and biochemical tests). Conversely, screening techniques demand a quick and low-cost grouping of bacterial/fungal isolates and current analysis call for broad reports of microorganisms, involving the application of molecular techniques (e.g., 16S ribosomal RNA gene sequencing based on polymerase chain reaction). The goal of this review is to present the past and the present methods of detection and identification of microorganisms, and to discuss their advantages and their limitations.

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A comparative study of the BBL crystal enteric/nonfermenter identification system and the biomerieux API20E and API20NE identification systems after overnight incubation

Cited by 10 publications

References 9 publications

Microbiological and Clinical Aspects of Infection Associated with Stenotrophomonas maltophilia

Microbiological and Clinical Aspects of Infection Associated with Stenotrophomonas maltophilia

Identification of Motile Aeromonas Strains with the MicroScan WalkAway System in Conjunction with the Combo Negative Type 1S Panels

Advances in Chemical and Biological Methods to Identify Microorganisms—From Past to Present

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