A comparative study of the antimicrobial and antioxidant activities of
            <i>Inonotus hispidus</i>
            fruit and their mycelia extracts

Angelini, Paola; Girometta, Carolina Elena; Tirillini, Bruno; Moretti, Simone; Covino, Stefano; Cipriani, Melania; D'Ellena, Eleonora; Giancarlo, Angeles; Federici, Ermanno; Savino, Elena; Cruciani, Gabriele; Venanzoni, Roberto

doi:10.1080/10942912.2019.1609497

Cited by 42 publications

(35 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ten compounds were identified from a suspect list compiled using scientific literature for mushrooms [ 22 , 25 , 27 , 28 , 29 , 30 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ] and were identified in both the raw material (mushroom straw) and the corresponding final product. The procedure for determining these compounds is briefly outlined below.…”

Section: Discussionmentioning

confidence: 99%

From By-Products to Fertilizer: Chemical Characterization Using UPLC-QToF-MS via Suspect and Non-Target Screening Strategies

2022

View full text Add to dashboard Cite

The increasing demands of agriculture and the food market have resulted in intensive agricultural practices using synthetic fertilizers to maximize production. However, significant efforts have been made to implement more environmentally friendly procedures, such as composting, to overcome the adverse impact of these invasive practices. In the terms of this research, composting was applied to the production of two biofertilizers, using onion and mushroom by-products as raw materials respectively. The main purposes of this work were to identify the compounds that pass from the raw materials to the final products (onion-based and mushroom-based), as well as the characterization of the chemical profile of these final products following suspect and non-target screening workflows via UPLC-qToF-MS. Overall, 14 common compounds were identified in the onion and its final product, while 12 compounds were found in the mushroom and its corresponding product. These compounds belong to fatty acids, organic acids, and flavonoids, which could be beneficial to plant health. The determination of parameters, such as the pH, conductivity, organic matter, nitrogen content, and elemental analysis, were conducted for the overall characterization of the aforementioned products.

show abstract

Section: Discussionmentioning

confidence: 99%

From By-Products to Fertilizer: Chemical Characterization Using UPLC-QToF-MS via Suspect and Non-Target Screening Strategies

2022

View full text Add to dashboard Cite

show abstract

“…Genomic DNA was isolated from 15-day old mycelium grown in Malt Extract Agar (Sigma−Aldrich, Milan, Italy), according to [ 31 ]. The genomic DNA quality and quantity was evaluated by BIO-RAD model 200/2.0 Power Supply gel electrophoresis [0.8% agarose gel in 1 × TBE buffer (89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 7.6)] in the presence of SafeView Nucleic Acid Stain (NBS Biologicals) and a MassRuler DNA Ladder, Mix (Thermo Scientific, Lithuania), and visualized with Safe ImagerTM 2.0 Blue-Light Transilluminator Invitrogen (Thermo Fisher Scientific, Israel); DNA samples were consequently diluted by up to 10 μg/μL with nuclease-free water before PCR amplification.…”

Section: Methodsmentioning

confidence: 99%

“…The genomic DNA quality and quantity was evaluated by BIO-RAD model 200/2.0 Power Supply gel electrophoresis [0.8% agarose gel in 1 × TBE buffer (89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 7.6)] in the presence of SafeView Nucleic Acid Stain (NBS Biologicals) and a MassRuler DNA Ladder, Mix (Thermo Scientific, Lithuania), and visualized with Safe ImagerTM 2.0 Blue-Light Transilluminator Invitrogen (Thermo Fisher Scientific, Israel); DNA samples were consequently diluted by up to 10 μg/μL with nuclease-free water before PCR amplification. The internal transcribed spacer (ITS) region was amplified through polymerase chain reactions (PCRs) with ITS1F and ITS4 primers, according to Angelini et al [ 31 ]. SimpliAmp Thermal Cycler AppliedBiosystems (Thermo Fisher Scientific, Singapore) was programmed as follows: one cycle of denaturation at 95 °C for 2.5 min; 35 cycles of denaturation at 95 °C for 20 s, annealing at 55 °C for 20 s and extension at 72 °C for 45 s; one final extension cycle at 72 °C for 7 min.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Antioxidant, Antimicrobial and Tyrosinase Inhibitory Activities of Extracts from Tricholosporum goniospermum, an Edible Wild Mushroom

et al. 2020

Self Cite

View full text Add to dashboard Cite

Tricholosporum goniospermum (Bres.) Guzmán ex T.J. Baroni is an excellent edible mushroom whose compounds and biological properties are still unknown. In this study, n-hexane, ethyl acetate and methanol extracts from fruiting bodies and liquid-cultured mycelia were compared for the analysis of phenolic compounds, the evaluation of scavenger (DPPH, ABTS) and reducing (CUPRAC, FRAP) activities, and the enzyme inhibition of α-amylase, acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase. Additionally, T. goniospermum extracts were evaluated for antibacterial and antimycotic activities against Gram+ and Gram− bacteria, and clinical yeast and fungal dermatophytes. Finally, based on the extract content in phenolic compounds, in silico studies, including the docking approach, were conducted to predict the putative targets (namely tyrosinase, lanosterol-14-α-demethylase, the multidrug efflux system transporters of E. coli (mdtK) and P. aeruginosa (pmpM), and S. aureus β-lactamase (ORF259)) underlying the observed bio-pharmacological and microbiological effects. The methanolic extract from mycelia was the richest in gallic acid, whereas the ethyl acetate extract from fruiting bodies was the sole extract to show levels of catechin. Specifically, docking runs demonstrated an affinity of catechin towards all docked proteins, in the micromolar range. These in silico data are consistent, at least in part, with the highest activity of ethyl acetate extract as an antimicrobial and anti-tyrosinase (554.30 mg KAE/g for fruiting bodies and 412.81 mg KAE/g for mycelia) agent. The ethyl acetate extracts were also noted as being the most active (2.97 mmol ACAE/g for fruiting bodies and 2.25 mmol ACAE/g for mycelia) on α-amylase. BChE inhibitory activities varied from 2.61 to 26.78 mg GALAE/g, while the tested extracts were not active on AChE. In conclusion, all mushroom extracts tested in this study had potent antimicrobial activities. Particularly, among the tested extracts, the ethyl acetate extract showed the highest efficacy as both an antimicrobial and anti-tyrosinase agent. This could be related, albeit partially, to its content of catechin. In this regard, the bioinformatics analyses showed interactions of catechin with tyrosinase and specific microbial proteins involved in the resistance to chemotherapeutic drugs, thus suggesting innovative pharmacological applications of T. goniospermum extracts.

show abstract

“…Wood decay fungi include several edible species as well as species reported to be the source of bioactive compounds, related to either primary (e.g., β-glucans) or secondary metabolism (terpenoids, phenolics, acids, superior alcohols, etc.). Structural diversity, occurrence and distribution among taxa, synthesis stimulation factors and correlation to growth stage, bioactivity pathways, standardization of products and crude extracts are the main current topics under investigation [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

An Italian Research Culture Collection of Wood Decay Fungi

et al. 2020

Self Cite

View full text Add to dashboard Cite

One of the main aims of the University of Pavia mycology laboratory was to collect wood decay fungal (WDF) strains in order to deepen taxonomic studies, species distribution, officinal properties or to investigate potential applications such as biocomposite material production based on fungi. The Italian Alps, Apennines and wood plains were investigated to collect Basidiomycota basidiomata from living or dead trees. The purpose of this study was to investigate the wood decay strains of the Mediterranean area, selecting sampling sites in North and Central Italy, including forests near the Ligurian and Adriatic seas, or near the Lombardy lakes. The isolation of mycelia in pure culture was performed according to the current methodology and the identity of the strains was confirmed by molecular analyses. The strains are maintained in the Research Culture Collection MicUNIPV of Pavia University (Italy). Among the 500 WDF strains in the collection, the most interesting isolates from the Mediterranean area are: Dichomitus squalens (basidioma collected from Pinus pinea), Hericium erinaceus (medicinal mushroom), Inocutis tamaricis (white-rot agent on Tamarix trees), Perenniporia meridionalis (wood degrader through Mn peroxidase) and P. ochroleuca. In addition, strains of species related to the Mediterranean climate (e.g., Fomitiporia mediterranea and Cellulariella warnieri) were obtained from sites with a continental-temperate climate.

show abstract

A comparative study of the antimicrobial and antioxidant activities of Inonotus hispidus fruit and their mycelia extracts

Cited by 42 publications

References 55 publications

From By-Products to Fertilizer: Chemical Characterization Using UPLC-QToF-MS via Suspect and Non-Target Screening Strategies

From By-Products to Fertilizer: Chemical Characterization Using UPLC-QToF-MS via Suspect and Non-Target Screening Strategies

Evaluation of Antioxidant, Antimicrobial and Tyrosinase Inhibitory Activities of Extracts from Tricholosporum goniospermum, an Edible Wild Mushroom

An Italian Research Culture Collection of Wood Decay Fungi

Contact Info

Product

Resources

About