A comparative study of the determination of phosphorus by electrothermal atomic absorption spectrometry and solution spectrophotometry

Lin, Shao-wen; Julshamn, Kaare

doi:10.1016/s0003-2670(00)84827-8

Cited by 10 publications

(2 citation statements)

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“…Mineral elements in spinach have been reported by other studies employing a large number of techniques [ 46 – 51 ]. On a moisture-free basis, the highest levels of K, Ca, Na, P, Mg, S, Zn, Cu, Co, and B were found in spinach compared to parsley, dill, and mint [ 52 ].…”

Section: Discussionmentioning

confidence: 69%

Genetic diversity and association mapping of mineral element concentrations in spinach leaves

et al. 2017

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BackgroundSpinach is a useful source of dietary vitamins and mineral elements. Breeding new spinach cultivars with high nutritional value is one of the main goals in spinach breeding programs worldwide, and identification of single nucleotide polymorphism (SNP) markers for mineral element concentrations is necessary to support spinach molecular breeding. The purpose of this study was to conduct a genome-wide association study (GWAS) and to identify SNP markers associated with mineral elements in the USDA-GRIN spinach germplasm collection.ResultsA total of 14 mineral elements: boron (B), calcium (Ca), cobalt (Co), copper (Cu), iron (Fe), potassium (K), magnesium (Mg), manganese (Mn), molybdenum (Mo), sodium (Na), nickel (Ni), phosphorus (P), sulfur (S), and zinc (Zn) were evaluated in 292 spinach accessions originally collected from 29 countries. Significant genetic variations were found among the tested genotypes as evidenced by the 2 to 42 times difference in mineral concentrations. A total of 2402 SNPs identified from genotyping by sequencing (GBS) approach were used for genetic diversity and GWAS. Six statistical methods were used for association analysis. Forty-five SNP markers were identified to be strongly associated with the concentrations of 13 mineral elements. Only two weakly associated SNP markers were associated with K concentration. Co-localized SNPs for different elemental concentrations were discovered in this research. Three SNP markers, AYZV02017731_40, AYZV02094133_57, and AYZV02281036_185 were identified to be associated with concentrations of four mineral components, Co, Mn, S, and Zn. There is a high validating correlation coefficient with r > 0.7 among concentrations of the four elements. Thirty-one spinach accessions, which rank in the top three highest concentrations in each of the 14 mineral elements, were identified as potential parents for spinach breeding programs in the future.ConclusionsThe 45 SNP markers strongly associated with the concentrations of the 13 mineral elements: B, Ca, Co, Cu, Fe, Mg, Mn, Mo, Na, Ni, P, S, and Zn could be used in breeding programs to improve the nutritional quality of spinach through marker-assisted selection (MAS). The 31 spinach accessions with high concentrations of one to several mineral elements can be used as potential parents for spinach breeding programs. Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4297-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 69%

Genetic diversity and association mapping of mineral element concentrations in spinach leaves

et al. 2017

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“…Saeed e Thomassen 16 listaram 21 elementos que melhoram a sensibilidade do fósforo, mas os dados foram baseados em altura de pico, não refletindo, portanto, a eficiência da atomização. A modificação da superfície do tubo de grafite com zircônio também foi proposta por alguns autores 2,17,18 como alternativa à adição de um modificador.…”

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Determinação de fósforo em aços por espectrometria de absorção atômica no forno de grafite

Curtius

1998

Quím. Nova

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steel samples by graphite furnace atomic absorption spectrometry, using the Zeeman effect background correction and the STPF conditions is proposed. The effect of iron (III) on the phosphorus signal was studied. It was verified, through the pyrolysis temperature curves, that iron (III) is an efficient chemical modifier, thermically stabilizing phosphorus up to 1400 o C. The phosphorus signal increases with the iron (III) concentration, but in the range, which corresponds to the usual concentrations of iron in the sample solutions, the increase is small. Phosphorus was determined in three standard reference materials, after its dissolution in a mixture of hydrochloric and perchloric acids in a PTFE bomb. The agreement with the certified concentration values was excellent. Iron (III) was added to the reference analytical solutions prepared in the blank of the dissolution, while the sample solutions were measured directly, since they already contained the modifier. The detection limit (k = 2) was 0.0042% of phosphorus in the steel sample.Keywords: graphite furnace atomic absorption spectrometry; steel; phosphorus. ARTIGO INTRODUÇÃOA espectrometria de absorção atômica no forno de grafite (GFAAS) é pouco utilizada na determinação de fósforo. Apenas quatro referências foram encontradas na literatura [1][2][3][4] , sobre a determinação de fósforo em aços e ligas, após dissolução ácida das amostras. Uma das razões, é que as linhas de ressonância para este elemento estão na região entre 167,2 e 178,8 nm, não utilizável em instrumentos convencionais, por causa da necessidade de vácuo ou purga com um gás inerte. L'vov e o C. Ainda assim, neste dublete, a sensibilidade é cerca de 100 vezes menor do que nas linhas de ressonância 6 . Além disso, Persson e Frech 7 observaram, através de cálculos teóricos e dados experimentais, perdas de fósforo antes da atomização, na forma de sub-óxidos e/ou moléculas do dímero de modo que para obter resultados reprodutíveis, recomendaram controle da temperatura final do forno e também da atmosfera dentro do tubo de grafite. Para diminuir as perdas e aumentar a sensibilidade, é importante a formação de uma fase termicamente estável. O grafite eletrolítico, mais ativo do que o pirolítico, e principalmente ativado com gás oxigênio, é capaz de estabilizar o fós-foro, conforme verificado por este autor e colaboradores 8 . No entanto, ainda assim, grandes quantidades de fósforo são perdidas antes da atomização, de modo que uma determinação sem um modificador químico não tem importância prática. Ediger 9 propôs o uso de lantânio como modificador para fósfo-ro, que foi usado em algumas aplicações analíticas 4,10-12 . Ní-quel também foi usado como modificador 3,13 , já que havia se mostrado eficiente na determinação do análogo arsênio 14,15 . Saeed e Thomassen 16 listaram 21 elementos que melhoram a sensibilidade do fósforo, mas os dados foram baseados em altura de pico, não refletindo, portanto, a eficiência da atomização. A modificação da superfície do tubo de grafite com zircônio também foi propos...

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Bioactive bisbenzylisoquinoline alkaloids from the roots of Stephania tetrandra

Wang

Liu

Shi

et al. 2020

Bioorganic Chemistry

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A comparative study of the determination of phosphorus by electrothermal atomic absorption spectrometry and solution spectrophotometry

Cited by 10 publications

References 9 publications

Genetic diversity and association mapping of mineral element concentrations in spinach leaves

Genetic diversity and association mapping of mineral element concentrations in spinach leaves

Determinação de fósforo em aços por espectrometria de absorção atômica no forno de grafite

Bioactive bisbenzylisoquinoline alkaloids from the roots of Stephania tetrandra

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