A comparative study of the contraction induced by high K/Na deficient solution in the trachea, gall bladder and urinary bladder in guinea-pig.

Shimizu, Kazumasa; Yaguchi, Shin‐ichi; Kometani, Atsuko; Nakajyo, Shinjiro; Ozaki, Hiroshi; Urakawa, Norimoto

doi:10.1254/jjp.34.231

Cited by 16 publications

(32 citation statements)

References 15 publications

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“…After incubation, the strips were occasionally removed from the organ bath, blotted on filter paper to exclude adhering solution and weighed using a balance. The ratio of cellular water content was calculated using the following equation [17]:…”

Section: Muscle Preparations and Tension Measurementmentioning

confidence: 99%

“…Since the ECS of the bovine trachea was not determined in the present experiments, a value reported in the literature was used in the calculation; the value of ECS in the guinea pig trachea was reported to be 0.56 [17]. To determine the dry weight of the strips, the samples were incubated in PSS or test solution and the was removed, blotted, weighted, dried at 95°C for 20 hr in vessels, and then weighed again.…”

Section: Muscle Preparations and Tension Measurementmentioning

confidence: 99%

“…Application of hyperosmotically-added high-K + solution induced a sustained contraction, while an isosmotically-substituted high-K + , Na + -deficient solution induces contraction followed by gradual relaxation in various smooth muscles [10,11,[17][18][19][21][22][23][24]. The possible mechanism of these changes in muscle contractility of tonic muscle is swelling of the cells, as in rabbit aorta [21] and guinea pig trachea [17].…”

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confidence: 99%

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confidence: 99%

“…The possible mechanism of these changes in muscle contractility of tonic muscle is swelling of the cells, as in rabbit aorta [21] and guinea pig trachea [17]. On the other hand, the changes in phasic muscle are due to an inhibition of glucose utilization resulting from Na + deficiency in medium, as in guinea pig [17] and rat [10] urinary bladders, and the guinea pig ileum [19] and vas deferens [18]. However, the changes in some smooth muscles are due to both swelling and inhibition of glucose utilization, as in the rabbit trachea [23] and the guinea pig gall bladder [17], seminal vesicle [18], and taenia coli [21].…”

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Effects of High-K+, Na+-Deficient Solution on Contractility of the Smooth Muscles of the Bovine Trachea

Kaneda

SAKAGUCHI

Shimizu

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. A high-K + , Na + -deficient (I-154 K + ) solution induced contraction followed by gradual relaxation of the smooth muscles of the bovine trachea, while hyperosmotic addition of 65 mM KCl induced a large sustained contraction. Exposure of the muscle to the I-154 K + solution induced an increase in the ratio of cellular water content and a sustained increase in oxidized flavoprotein fluorescence or reduced pyridine nucleotide fluorescence. The I-154 K + solution also induced a sustained increase in [Ca 2+ ]i level. Decreases in developed tension and increases in cellular water content were both prevented by the addition of sucrose or NaCl but not pyruvate. Substitution of KI for KCl in the I-154 K + solution produced a greater inhibition of contraction, while substitution with K-propionate produced no inhibition of contraction. Moreover, decreases in developed tension and increases in cellular water content were both prevented by addition of 100 µM 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS), but not 10 µM bumetanide or 1 mM acetazolamide. In conclusion, I-154 K + solution induced-relaxation in the bovine trachea may be due to swelling of smooth muscle cells and the mechanism of swelling is probably involved in DIDS-sensitive anion movement.

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Section: Muscle Preparations and Tension Measurementmentioning

confidence: 99%

Section: Muscle Preparations and Tension Measurementmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of High-K+, Na+-Deficient Solution on Contractility of the Smooth Muscles of the Bovine Trachea

Kaneda

SAKAGUCHI

Shimizu

et al. 2006

The Journal of Veterinary Medical Science

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Origin of ATP for Ca 2+ -induced contraction in the guinea-pig femoral artery

Takeuchi

Kushida

Kitayama

et al. 2004

Naunyn-Schmiedeberg's Archives of Pharmacology

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Previously, we have described differences between the rat proximal colon and femoral artery with respect to the role of ATP newly synthesized by creatine kinase. In the present study the role of newly synthesized ATP was studied in the guinea-pig femoral artery to examine species differences. In the alpha-toxin-permeabilized preparation of the guinea-pig femoral artery, the rapid Ca(2+)-induced contraction was suppressed when creatine kinase activity was inhibited. The contraction was restored completely by treatment with NaN(3), an inhibitor of ecto-ATPase, the enzyme that breaks down exogenous ATP. Thus, ATP newly synthesized by creatine kinase may have no role in contraction of the guinea-pig femoral artery. This is in marked contrast to the rat femoral artery, in which Ca(2+)-induced contractions are almost completely inhibited by inhibition of creatine kinase activity but only partly restored by NaN(3). To characterize the difference between the guinea-pig and rat tissue, the origin of ATP required for contraction was determined in intact preparations. Monoiodoacetic acid, an inhibitor of glycolysis, inhibited the high K(+)-induced contraction in the guinea-pig femoral artery more potently than in the rat tissue. In contrast, an inhibitor of mitochondrial respiration, carbonylcyanide p-(trifluoromethoxy)phenylhydrazone (FCCP), inhibited contraction in femoral arteries from rats, but not from guinea-pigs. These results suggest that contraction in the rat femoral artery is dependent largely on oxidative phosphorylation, while contraction in the guinea-pig tissue is dependent only on glycolysis. Because oxidative phosphorylation generates ATP and phosphocreatine, while glycolysis generates only ATP, the strong dependence of the contraction of the rat femoral artery on the oxidative phosphorylation is consistent with its dependence on ATP newly synthesized by creatine kinase from ADP and phosphocreatine, as previously shown. Thus, it is proposed that ATP, newly synthesized by creatine kinase, in addition to ATP generated by oxidative phosphorylation, is utilized for contraction in the rat femoral artery, while glycolysis produces sufficient ATP for contraction in the guinea-pig femoral artery.

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Inhibitory Mechanism of Papaverine on the Smooth Muscle of Guinea Pig Urinary Bladder

Shimizu¹,

Ichikawa²,

Urakawa³

et al. 2000

Japanese Journal of Pharmacology

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A comparative study of the contraction induced by high K/Na deficient solution in the trachea, gall bladder and urinary bladder in guinea-pig.

Abstract: Abstract-In

Cited by 16 publications

References 15 publications

Effects of High-K+, Na+-Deficient Solution on Contractility of the Smooth Muscles of the Bovine Trachea

Effects of High-K+, Na+-Deficient Solution on Contractility of the Smooth Muscles of the Bovine Trachea

Origin of ATP for Ca 2+ -induced contraction in the guinea-pig femoral artery

Inhibitory Mechanism of Papaverine on the Smooth Muscle of Guinea Pig Urinary Bladder

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