A comparative study of the effects of laser and light-emitting diode radiation on superoxide dismutase activity and nitric oxide production in rat wound fluid

Klebanov, G. I.; Shuraeva, N. Yu.; Chichuk, T. V.; Осипов, А. Н.; Vladimirov, Yu. A.

doi:10.1134/s0006350906010143

Cited by 11 publications

(8 citation statements)

References 22 publications

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“…The minor differences in bacterial growth suppression between the LED and laser did not reach statistical significance in 35 of the 36 experimental trials involving three Overall, our results are in agreement with those studies which have compared the effects of other wavelengths of LEDs and lasers on wound healing and cell migration and found no difference between the two [35]. Thus, as noted in other studies [13,[35][36][37], coherence does not seem to play a significant role in cellular response to irradiation with light; rather, fluence and wavelength exert a significant effect of treatment outcome [35]. The similarity between the antimicrobial effects of our two light sources on MRSA suggests that coherence also does not play a role in bacterial growth suppression.…”

Section: Discussionsupporting

confidence: 94%

The relative antimicrobial effect of blue 405 nm LED and blue 405 nm laser on methicillin-resistant Staphylococcus aureus in vitro

et al. 2015

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It has long been argued that light from a laser diode is superior to light from a light-emitting diode (LED) in terms of its effect on biological tissues. In order to shed light on this ongoing debate, we compared the antimicrobial effect of light emitted from a 405-nm LED with that of a 405-nm laser on methicillin-resistant Staphylococcus aureus (MRSA) at comparable fluences. We cultured 5 × 10(6) CFU/ml MRSA on tryptic soy agar and then irradiated culture plates once, twice, or thrice with either LED or laser light using 40, 54, 81, or 121 J/cm(2) fluence at 15-, 30-, or 240-min time interval between irradiation. Cultures were incubated immediately after irradiation at 37 °C for 24 h before imaging and counting remnant bacterial colonies. Regardless of the device used, LED or laser, irradiation at each fluence resulted in statistically significant bacterial growth suppression compared to non-irradiated controls (p < 0.0001). The antimicrobial effect of both light sources, LED and laser, was not statistically different at each fluence in 35 of the 36 experimental trials. Bacterial growth suppression achieved with either source of light increased with repeated irradiation, particularly at the 15- or 30-min treatment time interval. Thus, we conclude that the antimicrobial effect of 405-nm laser and 405-nm LED on MRSA is similar; neither has a superior antimicrobial effect when compared to the other.

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Section: Discussionsupporting

confidence: 94%

The relative antimicrobial effect of blue 405 nm LED and blue 405 nm laser on methicillin-resistant Staphylococcus aureus in vitro

et al. 2015

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“…The data accumulated up to the present are insufficient to make any definitive statement on the role of the polarization of light in the enhanced development of cellular and systemic effects in human subjects postirradiation (8,31,42,52,57,58). On the basis of available data, including the current study, it can therefore be suggested that not only polarized but also nonpolarized visible and IR light in the terrestrial solar waveband can possibly produce positive effects on the state of the immune system, wound repair and general homeostasis in human subjects.…”

Section: Discussionmentioning

confidence: 99%

Polychromatic Light Similar to the Terrestrial Solar Spectrum Without its UV Component Stimulates DNA Synthesis in Human Peripheral Blood Lymphocytes In Vivo and In Vitro

Zhevago

Samoilova

Calderhead

2006

Photochem & Photobiology

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Immunosuppressive effects of the minor component of the terrestrial solar spectrum, UV radiation, have been substantiated over the past several years. This raises the question of what influence the dominant part of the solar spectrum--visible and IR light-would have on the human immune system. In the present randomized, placebo-controlled double-blind study a small area of the body surface of volunteers was irradiated with polychromatic light (480-3400 nm), simulating the significant part of the terrestial sunlight irradiance spectrum and its power density. An average 2.5-fold to three-fold increase in spontaneous and phytohemagglutinin-induced DNA synthesis in peripheral blood lymphocytes (Lym) was revealed at 0.5-24 h after irradiation at a therapeutic dose (12 J/cm(2)) in subjects with low preirradiation levels of both processes. The in vivo findings were echoed in parallel in vitro experiments, when blood drawn from the same subjects was directly irradiated (2.4 J/cm(2)), or when the irradiated blood was mixed 1:10 with nonirradiated autologous blood to model events in the circulation following transcutaneous blood photomodification. Our data suggest that exposure of the human body to polychromatic visible + IR light may photomodify blood in the dermal vasculature of the irradiated area to lead to an immediate transfer of the light-induced effects to Lym of the entire circulating blood, which can result in modulation of Lym functional state at the systemic level.

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“…healing of excision wound in the rat. The parameters of LED irradiation used in this study are based on previous studies 7,10,11. LED irradiation was increased the wound closure rate and colla-gen density in situ at day 7 post excision wound, This result suggest that LED irradiation stimulate the fibroblast proliferation and collagen deposition in the granulation tissue.…”

mentioning

confidence: 83%

“…23 The radiation coherence does not play important role during wound healing process. 5,7 Furthermore, LED arrays can be irradiated in large areas and can minimize the risk of tissue damage. Also LED is inexpensive than laser.…”

Section: J Kptmentioning

confidence: 99%

“…6 The major difference between LED and laser light is coherency. The radiation coherence does not play any significant role in the cellular activity during wound healing process, 7 and coherence does not seem essential to the effects of laser phototherapy. 5 While many studies for LLLT in wound healing have been reported, relatively not much research has been reported for LED in wound healing.…”

Section: Introductionmentioning

confidence: 99%

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630 nm Light Emitting Diode Irradiation Improves Dermal Wound Healing in Rats

Jekal

Kwon

2015

J Kor Phys Ther

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Purpose: To determine the effects of 630 nm light emitting diode (LED) on full-thickness wound healing. Methods: Twelve male Sprague-Dawley rats were randomly divided into LED (n= 6) and control group (n= 6). Two 19.63 mm 2 wounds were created on the mid dorsum. LED group received a 630 nm LED irradiation with 3.67 mW/cm 2 for 30 minutes (6.60 J/cm 2) for 7 days, while control group received sham LED irradiation. Epithelial gap, collagen density, α-SMA fibroblast and PCNA keratinocyte were measured on histochemical and immunohistochemical staining using image analysis system. An independent t-test was conducted to compare the difference between groups. Results: The wound closure rate, collagen density, α-SMA fibroblast number, epithelial gap and PCNA keratinocyte number have shown no significant difference between LED and control group at day 3 after the treatment. At day 7 after the treatment, the wound closure rate in LED group was increased when compared with control group (p< 0.05). The collagen density (p< 0.05) and α-SMA immunoreactive fibroblast number (p< 0.001) were increased when compared with control group at day 7. The epithelial gap in LED group was significantly shorten than control group at day 7 (p< 0.01). The PCNA positive cell number in LED group was higher than control group at day 7 (p< 0.01). Conclusion: 630 nm LED with 3.67 mW/cm 2 , 6.60 J/cm 2 accelerate collagen deposition by stimulating fibroblasts, and enhance wound contraction by differentiating myofibroblasts in the dermis, and accelerate keratinocyte proliferation by facilitating DNA synthesis in the epidermis. It may promote the healing process in proliferation stage of wound healing.

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A comparative study of the effects of laser and light-emitting diode radiation on superoxide dismutase activity and nitric oxide production in rat wound fluid

Cited by 11 publications

References 22 publications

The relative antimicrobial effect of blue 405 nm LED and blue 405 nm laser on methicillin-resistant Staphylococcus aureus in vitro

The relative antimicrobial effect of blue 405 nm LED and blue 405 nm laser on methicillin-resistant Staphylococcus aureus in vitro

Polychromatic Light Similar to the Terrestrial Solar Spectrum Without its UV Component Stimulates DNA Synthesis in Human Peripheral Blood Lymphocytes In Vivo and In Vitro

630 nm Light Emitting Diode Irradiation Improves Dermal Wound Healing in Rats

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