A comparative study of the HPLC-MS profiles and biological efficiency of different solvent leaf extracts of two African plants: <i>Bersama abyssinica</i> and <i>Scoparia dulcis</i>

Sinan, Kouadio Ibrahime; Béné, Kouadio; Zengin, Gökhan; Diuzheva, Alina; Jekő, József; Cziáky, Zoltán; Picot-Allain, Marie Carene Nancy; Mollica, Adriano; Rengasamy, Kannan R.R.; Mahomoodally, Mohamad Fawzi

doi:10.1080/09603123.2019.1652885

Cited by 12 publications

(22 citation statements)

References 33 publications

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“…On the other hand, the ethyl acetate extract (15.22 mmol ACAE/g extract) showed potent activity against α-glucosidase. It is noteworthy that B. abyssinica stem bark ethyl acetate extract was more active than the ethyl acetate extract of B. abyssinica leaves (1.09 mmol ACAE/g extract) [21].…”

Section: Phytochemical Profilementioning

confidence: 86%

“…The metal chelating evaluation showed that the water extract was the most active. With reference to a previously published paper, B. abyssinica stem bark (Table 1) assessed in this study possessed a higher level of phenolic as compared with B. abyssinica leaves (36.99, 175.95, and 180.62 mg GAE/g extract for ethyl acetate, methanol, and water extracts, respectively) [21]. In addition to spectrophotometric determination of secondary metabolites, the detailed profiles were elucidated using HPLC-fluorimetric technique.…”

Section: Phytochemical Profilementioning

confidence: 87%

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Biopotential of Bersama abyssinica Fresen Stem Bark Extracts: UHPLC Profiles, Antioxidant, Enzyme Inhibitory, and Antiproliferative Propensities

et al. 2020

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In this study, ethyl acetate, methanol, and water extracts of Bersama abyssinica (Melianthaceae) stem bark were screened for enzyme inhibitory and antioxidant properties. The water extract possessed the highest concentration of phenols (230.83 mg gallic acid equivalent/g extract), while the methanol extract was rich in flavonoids (75.82 mg rutin equivalent/g extract), and the ethyl acetate extract possessed the highest amount of saponins (97.37 mg quillaja equivalent/g). The aim of this study was to investigate the antiproliferative effects against the human colon cancer HCT116 cell line challenged with serotonin (5-HT) as a stimulating-proliferation factor. The level of HCT116 cell-deriving pool of kynurenic acid (KA) was also assessed. The UHPLC results confirmed the presence of 58, 68, and 63 compounds in the ethyl acetate, methanol, and water extracts, respectively. Mangiferin, vitexin and its isomer isovitexin were tentatively identified in all extracts and KA (m/z 190.05042 [M−H] + ) was also tentatively identified in the methanol and water extracts. The methanol extract (1464.08 mg Trolox equivalent [TE]/g extract) showed the highest activity in the CUPRAC assay, whereas the water extract (1063.70 mg TE/g extract) showed the highest activity with the FRAP technique. The ethyl acetate extract was the most active acetylcholinesterase (4.43 mg galantamine equivalent/g extract) and α-glucosidase (mmol acarbose equivalent /g extract) inhibitor. The water extract was able to inhibit 5-HT-stimulated viability of HCT116 cells, and blunt 5-HT-induced reduction of cell-deriving KA. The scientific data generated in this study provide baseline data regarding the biological properties of B. abyssinica stem bark, highlighting its potential use for the development of new pharmaceutic and cosmetic agents.

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Section: Phytochemical Profilementioning

confidence: 86%

Section: Phytochemical Profilementioning

confidence: 87%

Biopotential of Bersama abyssinica Fresen Stem Bark Extracts: UHPLC Profiles, Antioxidant, Enzyme Inhibitory, and Antiproliferative Propensities

et al. 2020

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“…There are three diarrheal syndromes; acute diarrhea (250 ml/kg/day of water loss in the stool), bloody diarrhea (with visible blood in the stool), and persistent diarrhea (which lasts two weeks or more). Acute watery diarrhea with dehydration is the leading cause of death from diarrhea [4]. Every year, 1.7 billion cases of diarrhea in children and 525,000 deaths in under-age five children are reported.…”

Section: Introductionmentioning

confidence: 99%

“…About half of these diarrheal diseases are reported in South Asia and Africa. Children mortality rates from diarrhea are highest in sub-Saharan Africa [2,3], while Ethiopia has the fifth-highest diarrhea burden in the world [2,4].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the antidiarrheal activity of 80% methanol extract and solvent fractions of the leaf of Bersama abyssinica fresen (Melianthaceae) in mice

Ayalew

Bekele

Geta

et al. 2022

BMC Complement Med Ther

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Introduction The use of traditional medicinal plants in the management of diarrhea has long been practiced in Ethiopia. B. abyssinica fresen is one of the plants traditionally used to treat diarrhea whereas an in vivo study had not yet been conducted. Thus, this study aimed to evaluate the antidiarrheal activity of crude extract and solvent fractions of the leaf of B. abyssinica in mice. Methods Cold maceration within 80% methanol was used to extract the leaf powder and extract of the leaf was fractionated using n-hexane, chloroform, and distilled water. The in vivo antidiarrheal activity of crude extracts and solvent fractions were tested in experimental models of castor oil-induced diarrhea, enteropooling, and antimotility test. Five groups each with 6 mice were used under the three antidiarrheal models. Positive controls were treated with loperamide 3 mg/kg and atropine 5 mg/kg and 2% tween 80 was used in the treatment of negative controls. The extract and solvent fractions were administered at doses of 100, 200, and 400 mg/kg. Time of onset of diarrhea, number and weight of total and wet feces, the percent reduction in the number of wet feces, weight and volume of intestinal contents, and percent inhibition of intestinal motility were recorded. Data were analyzed using SPSS version 20. Result Defecation of castor oil-induced diarrheal or loose stools was inhibited (p < 0.01 to p < 0.001) at 200 mg/kg and 400 mg/kg of crude extract and aqueous fraction. The crude extract and the aqueous fraction at three doses (p < 0.01 to p < 0.001), the chloroform fraction at 200 mg/kg and 400 mg/kg (p < 0.01 to p < 0.001), and the n-hexane fraction at 400 mg/kg (p < 0.05) reduced intraluminal fluid accumulation compared with the negative control. Castor oil-induced intestinal motility was significantly suppressed with the three-doses of aqueous fraction (p < 0.05 to p < 0.001), 200 mg/kg and 400 mg/kg of crude extract (p < 0.05 to p < 0.01), 400 mg/kg of chloroform and n-hexane (p < 0.01 to p < 0.001) compared with negative control. Conclusion The crude extract, aqueous, and chloroform fractions of B. abyyssinica leaves have promising anti-diarrheal effects, supporting the plant's traditional use to treat diarrhea.

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Assessments of anticholinergic, antidiabetic, antioxidant activities and phenolic content of Stachys annua

Bursal

Taslimi

Gören

et al. 2020

Biocatalysis and Agricultural Biotechnology

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A comparative study of the HPLC-MS profiles and biological efficiency of different solvent leaf extracts of two African plants: Bersama abyssinica and Scoparia dulcis

Cited by 12 publications

References 33 publications

Biopotential of Bersama abyssinica Fresen Stem Bark Extracts: UHPLC Profiles, Antioxidant, Enzyme Inhibitory, and Antiproliferative Propensities

Biopotential of Bersama abyssinica Fresen Stem Bark Extracts: UHPLC Profiles, Antioxidant, Enzyme Inhibitory, and Antiproliferative Propensities

Evaluation of the antidiarrheal activity of 80% methanol extract and solvent fractions of the leaf of Bersama abyssinica fresen (Melianthaceae) in mice

Assessments of anticholinergic, antidiabetic, antioxidant activities and phenolic content of Stachys annua

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