A comparative study of the surface morphology of stimulated and unstimulated macrophages prepared without chemical fixation for scanning EM

Albrecht, Ralph M.; Hinsdill, Ronald D.; Sandok, P L; MacKenzie, Alan P.; Sachs, Irving B.

doi:10.1016/0014-4827(72)90203-0

Cited by 39 publications

(7 citation statements)

References 7 publications

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“…/. The same structures are reported to be observed frequently in activated tnacrophages (Albrecht et al 1972). They are composed of small, round/stellate eells intermingled with spindle-shaped fibroblast-like eells (x 100).…”

Section: Phase Contrast Mieroseopysupporting

confidence: 55%

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Tissue cultures of benign and malignant fibrous histiocytomas: SEM observations

Oku¹,

Takigawa²,

Fukamizu³

et al. 1984

J Cutan Pathol

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Four dermatofibromas (DF), 2 dermatofibrosarcomata protuberans (DFSP), 2 atypical fibroxanthmas of the skin (AFX), and one malignant fibrous histiocytoma (MFH) were studied by explant culture technique and scanning electron microscopy. Differences in the cellular atypism, phagocytic activity and motility were observed between histiocyte-like cells extending from a DF and DFSP group and an AFX and MFH group. Such cytological characteristics was maintained during in vitro transformation of the cells into fibroblastic cells. It was concluded that culture behaviors of the cells from each tumor group correlated well with in vivo growth and histologic features. We feel that examination of in vitro morphology of fibrous histiocytomas may prove useful in arriving at a correct diagnosis.

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Section: Phase Contrast Mieroseopysupporting

confidence: 55%

“…3A). The same structures are reported to be observed frequently in activated tnacrophages (Albrecht et al 1972). DFSP cells showed less pronounced membranous structures on the cell surface.…”

Section: #mentioning

confidence: 99%

Tissue cultures of benign and malignant fibrous histiocytomas: SEM observations

Oku¹,

Takigawa²,

Fukamizu³

et al. 1984

J Cutan Pathol

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“…On the other hand, peritoneal macrophages settling on the glass possessed, under the scanning electron microscope, thin fingerlike processes projecting out on all sides of the cell (CARR and CARR, 1970;ALBRECHT et al, 1972). The processes of macrophages in the spleen and peritoneal cavity may be homologous to the fibrous processes of the intraventricular macrophages in the present case.…”

Section: Discussionmentioning

confidence: 66%

Scanning Electron Microscope Observation of Intraventricular Macrophages (Kolmer Cells) in the Rat Brain

Hosoya

Fujita

1973

Arch. Histol. Jap., Arch. Histol. Jpn

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Summary. The choroid plexus and the third ventricle wall of the rat brain were observed by scanning electron microscopy.Macrophages of various shapes known as Kolmer cells were attached to the ependymal surface of the choroid plexus densely covered with microvilli. Some cells possessed numerous fine processes radiating from the centrally located cell body and ending, often with an attenuated web-like tip, among the ependymal microvilli. Others were cells with a few pseudopod-like processes of considerable thickness and length. The latter type was thought to be in locomotion.The same cells were occasionally also found in the third ventricle wall far from the choroid plexus. The Kolmer cells are thought to be scavengers belonging to the whole ventricular system, though they are gatherd mainly on the choroid plexus.Macrophage-like cells of wandering and phagocytotic nature were found by KOLMER (1921) on the choroid plexus of lower vertebrates. These cells, called Kolmer cells or, according to the designation by ARIENS-KAPPERS (1953), Epiplexuszellen, were later also found in mammals (see SCHALTENBRAND and DORN, 1955). Some transmission electron microscopic studies have been published on these cells (SANTOLAYA and ECHANDIA, 1968; CARPENTER, MCCARTHY and BORISON, 1970).Recently the scanning electron microscope has visualized the fine interior surface of the brain ventricles. The choroid plexus in the brain ventricle was observed with this microscope by SCOTT, PAULL and DUDLEY (1972) in man, by CLEMENTI and MARINI (1972) in the cat, by WEINDL and JOYNT (1972) in the monkey, cat and rabbit and by YAMADORI (1972) in the rat, but the Kolmer cells were not described by these authors.In the present study the choroid plexus of rat lateral ventricle was observed by scanning electron microscopy. This paper will provide a first and detailed description of the surface fine structure of the Kolmer cells with special reference to their functions as macrophages.The subsidial purpose of this paper is to present the fine surface view of the choroid plexus obtained by the critical point drying method as this valuable method was not applied in the previous, above-mentioned studies. Material and MethodMale rats weighing 300-400g were anesthetized with ether and perfused from the left ventricle with Ringer solution and then with a Karlsson-Schultz buffer containing 2.5% glutaraldehyde, 1% paraformaldehyde and 2% sucrose. The brain was carefully taken out to be fixed in the same fixative for at least one week. Tissue blocks including either the choroid plexus of the lateral ventricle or the wall of the third ventricle were excised from the brain. The specimens were thoroughly washed in 133

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“…They found the alteration of long and short microextension after trypsinization. ALBRECHT et al (1972) made a detailed examination of mouse macrophages using the freeze-drying method in place of the conventional procedure of air-drying or chemical fixation. The freeze-drying method improved surface visualization and reduced artifacts resulting from the shrinkage and collapse in air drying employed in the previous works.…”

Section: Discussionmentioning

confidence: 99%