A comparative study of skin irritation caused by novel bis-quaternary ammonium compounds and commonly used antiseptics by using cell culture methods

Yamamoto, Masashi; Matsumura, Reiko; Hirata, Yoshiharu; Nagamune, Hideaki

doi:10.1016/j.tiv.2018.09.009

Cited by 19 publications

(16 citation statements)

References 26 publications

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“…Mechanistic studies on PHMB concluded that it induced inflammatory responses through activation of nuclear factor kappa B activation and its signaling pathway [26]. The greater cytotoxicity of BZK ointment relative to PHMB ointment in our experiment was comparable to a previous study on the bioactive compounds [27]. MediHoney was less cytotoxic than BZK and PHMB and comparable to PG+CAP; however, MediHoney produced a greater reduction of normal metabolic activity relative to PG+CAP.…”

Section: Discussionsupporting

confidence: 77%

Enhanced Biofilm Eradication and Reduced Cytotoxicity of a Novel Polygalacturonic and Caprylic Acid Wound Ointment Compared with Common Antiseptic Ointments

Gerges

Rosenblatt

Truong

et al. 2021

BioMed Research International

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Antiseptic wound ointments are widely used to treat dermal wounds that are microbially contaminated. Polygalacturonic acid (PG)+caprylic acid (CAP) is a novel combination that has been shown to eradicate biofilms. We developed a novel PG+CAP ointment and compared the biofilm eradication capability and cytotoxicity of PG+CAP with that of commercially available antiseptic wound ointments. We used a well-established biofilm model to quantitatively assess the eradication of organisms following exposure to the wound ointments for 2 hours. PG+CAP ointment completely eradicated Candida albicans, multidrug-resistant Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus biofilms, whereas MediHoney, polyhexamethylene biguanide (PHMB), and benzalkonium chloride (BZK) ointments failed to eradicate all biofilms within 2 hours. We assessed cytotoxicity by exposing L-929 fibroblasts to extracts of each ointment; Trypan blue exclusion was used to assess cell viability, and Alamar blue conversion was used to assess metabolic function. After exposure to PG+CAP and MediHoney, fibroblast viability was 96.23% and 95.23%, respectively (Trypan blue), and was comparable to untreated cells (98.77%). PHMB and BZK showed reduced viability (83.25% and 77.83%, respectively, p < 0.05 ). Metabolic activity results followed a similar pattern. Cytotoxicity of PG+CAP ointment towards erythrocytes was comparable to saline. PG+CAP ointment seems to be safe and can rapidly eradicate microbial biofilm; thus, PG+CAP ointment merits further in vivo testing as a potential antimicrobial wound ointment.

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Section: Discussionsupporting

confidence: 77%

Enhanced Biofilm Eradication and Reduced Cytotoxicity of a Novel Polygalacturonic and Caprylic Acid Wound Ointment Compared with Common Antiseptic Ointments

Gerges

Rosenblatt

Truong

et al. 2021

BioMed Research International

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“…8e These compounds have same activity with octenidine (MIC and MBC), but better in terms of cytotoxicity (normal human epidermal keratinocytes). 9 Recently we have got structural analogues of 3PHBO-12, that contain either biphenyl-4,4 0 -diol 10a (3BP4BO-n,Hal; Fig. 2c), or 4,4 0 -oxydiphenol 10b (3OB4POB-n,Hal; Fig.…”

Section: Introductionmentioning

confidence: 99%

Investigations into the structure–activity relationship in gemini QACs based on biphenyl and oxydiphenyl linker

et al. 2021

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“…Using human keratinocytes (NHEK), Yamamoto et al (2019) 42 found that cell viability did not change significantly when cells were exposed to 2% PHMB solution for 15 min, corroborating with data from the same study in which the expression of the IL-1a-related inflammatory gene was substantially lower when compared to the expression of the same gene when NHEK cells were exposed to other antiseptic agents. Following the same trend of decreased cell viability as increased antiseptic concentration, Yabes et al (2017), 43 who tested the cytotoxic action of PHMB at different concentrations (0.01%, 0.04% and 0.1%) on human keratinocytes (HEK-001, ATCC CRL 2404), demonstrated that viability HEK-001 cells reached 8% rates after 24 hours of exposure to 0.1% PHMB solution, which is lower than the present study.…”

Section: Cell Viabilitymentioning

confidence: 56%

“…Using human keratinocytes (NHEK), Yamamoto et al. (2019) 42 found that cell viability did not change significantly when cells were exposed to 2% PHMB solution for 15 min, corroborating with data from the same study in which the expression of the IL-1α-related inflammatory gene was substantially lower when compared to the expression of the same gene when NHEK cells were exposed to other antiseptic agents. Following the same trend of decreased cell viability as increased antiseptic concentration, Yabes et al.…”

Section: Cell Viabilitymentioning

confidence: 56%

PHMB-loaded PDMS and its antimicrobial properties for biomedical applications

et al. 2021

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Given the global panorama of demands in the health area, the development of biomaterials becomes irreducible for the maintenance and/or improvement in the quality of life of the human being. Aiming to reduce the impacts related to infections in the healing processes of the dermal structure, the present work proposes the development of polydimethylsiloxane (PDMS) based membranes with the incorporated polyhexamethylenebiguanide (PHMB) antimicrobial agent. In the present study, the antimicrobial and antibiofilm properties of polydimethylsiloxane (PDMS) films incorporated with 0.1, 0.3, and 0.5% (w/w) of polyhexamethylene biguanide (PHMB) were evaluated, aiming the development of a protective biomaterial that avoids cutaneous infections from the autochthonous and allochthonous microbiota. The disk diffusion of PHMB-loaded PDMS has shown the growth inhibition of Escherichia coli (ATCC 9637), Pseudomonas aeruginosa (ATCC 27953), Acinetobacter baumannii (ATCC 19606), Staphylococcus aureus (ATCC 6538), Staphylococcus epidermidis (ATCC 12228), Streptococcus pyogenes (ATCC 19615), Bacillus subtilis (ATCC 6633) and also yeast-like fungi Candida albicans, all microorganisms found on the epidermal surface. Likewise, the present study demonstrated low cytotoxicity of the PHMB-loaded PDMS on HaCaT and L929 cells at lower concentrations (0.1% w/w), indicating the possibility of using the developed material as a dressing for wounds, burns, and post-surgical procedures.

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A comparative study of skin irritation caused by novel bis-quaternary ammonium compounds and commonly used antiseptics by using cell culture methods

Cited by 19 publications

References 26 publications

Enhanced Biofilm Eradication and Reduced Cytotoxicity of a Novel Polygalacturonic and Caprylic Acid Wound Ointment Compared with Common Antiseptic Ointments

Enhanced Biofilm Eradication and Reduced Cytotoxicity of a Novel Polygalacturonic and Caprylic Acid Wound Ointment Compared with Common Antiseptic Ointments

Investigations into the structure–activity relationship in gemini QACs based on biphenyl and oxydiphenyl linker

PHMB-loaded PDMS and its antimicrobial properties for biomedical applications

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