A comparative study of selective stimulation of raphe nuclei in the cat in inhibiting dorsal horn neuron responses to noxious stimulation

Griffith, James L.; Gatipon, Glenn B.

doi:10.1016/0006-8993(81)91015-5

Cited by 13 publications

(2 citation statements)

References 18 publications

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“…The dorsal raphe nucleus (DRN) is enriched in serotonin (5-hydroxytryptamine; 5-HT) neurons that play a critical role in the regulation of pain and affect (Akil and Mayer, 1972;Dugué et al, 2014;Griffith and Gatipon, 1981;Horie et al, 1991;Mayer and Liebeskind, 1974;Oliveras et al, 1979). Pro-inflammatory stimuli can precipitate 5-HT neuronal degeneration, induce cfos expression, and alter 5-HT neuronal activity in the DRN (Brambilla et al, 2007;Hochstrasser et al, 2011a;Hollis et al, 2006;Manfridi et (which was not certified by peer review) is the author/funder.…”

Section: Introductionmentioning

confidence: 99%

Adolescent ethanol drinking promotes hyperalgesia, neuroinflammation and serotonergic deficits in mice that persist into adulthood

Khan

Rosa

Biggerstaff

et al. 2023

Brain, Behavior, and Immunity

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Section: Introductionmentioning

confidence: 99%

Adolescent ethanol drinking promotes hyperalgesia, neuroinflammation and serotonergic deficits in mice that persist into adulthood

Khan

Rosa

Biggerstaff

et al. 2023

Brain, Behavior, and Immunity

View full text Add to dashboard Cite

“…The dorsal raphe nucleus (DRN) is enriched in serotonin (5-hydroxytryptamine; 5-HT) neurons that play a critical role in the regulation of pain and affect (Akil and Mayer, 1972;Dugué et al, 2014;Griffith and Gatipon, 1981;Horie et al, 1991;Mayer and Liebeskind, 1974;Oliveras et al, 1979). Pro-inflammatory stimuli can precipitate 5-HT neuronal degeneration, induce cfos expression, and alter 5-HT neuronal activity in the DRN (Brambilla et al, 2007;Hochstrasser et al, 2011a;Hollis et al, 2006;Manfridi et al, 2003), suggesting that chronic alcohol may promote hyperalgesia and negative affect by activating neuro-immune mechanisms in the DRN that ultimately perturb 5-HT neurotransmission.…”

Section: Introductionmentioning

confidence: 99%

Adolescent ethanol drinking promotes hyperalgesia, neuroinflammation and serotonergic deficits in mice that persist into adulthood

Khan

Rosa

Biggerstaff

et al. 2021

Preprint

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Adolescent alcohol use can permanently alter brain function and lead to poor health outcomes in adulthood. Emerging evidence suggests that alcohol use predispose to pain disorders or exacerbate existing pain conditions, but the neural mechanisms are currently unknown. Here we report that mice exposed to adolescent intermittent access to ethanol (AIE) exhibit increased pain sensitivity and depressive-like behaviors that persist after alcohol cessation and are accompanied by elevated CD68 expression in microglia and reduced numbers of serotonin (5-HT)-expressing neurons in the dorsal raphe nucleus (DRN). 5-HT expression was also reduced in the thalamus, anterior cingulate cortex (ACC) and amygdala as well as the lumbar dorsal horn of the spinal cord. We then found that chronic minocycline administration after AIE alleviated hyperalgesia and social deficits, while chemogenetic activation of microglia in the DRN of Cx3cr1-cre-GFP mice reproduced the effects of AIE on pain and social interaction. Taken together, these results indicate that microglial activation in the DRN may be a primary driver of pain and negative affect after AIE.

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Inhibition of the responses of cat dorsal horn neurons to noxious skin heating by stimulation in medial or lateral medullary reticular formation

1988

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Responses of single lumbar dorsal horn units to noxious radiant heating (50 degrees C, 10 s) of glabrous footpad skin were recorded in cats anesthetized with sodium pentobarbital and 70% nitrous oxide. The heat-evoked responses of 37/40 units were reduced during electrical stimulation (100 ms trains, 100 Hz, 3/s, 25-600 microA) in the medullary nucleus raphe magnus (NRM) and/or in laterally adjacent regions of the medullary reticular formation (MRF). Inhibition was elicited by stimulation in widespread areas of the medulla, but with greatest efficacy at ventrolateral sites. The magnitude of inhibition increased with graded increases in medullary stimulation intensity. Mean current intensities at threshold for inhibition or to produce 50% inhibition were higher for NRM than for MRF sites. Units' responses to graded noxious heat stimuli increased linearly from threshold (42-43 degrees C) to 52 degrees C. During NRM (5 units) or ipsilateral MRF stimulation (7 units), responses were inhibited such that the mean temperature-response functions were shifted toward higher temperatures with increased thresholds (1.5 degrees and 1 degree C, respectively) and reduced slopes (to 60% of control). Contralateral MRF stimulation had a similar effect in 4 units. Inhibitory effects of NRM and MRF stimulation were reduced (by greater than 25%) or abolished in 4/6 and 5/12 units, respectively, following systemic administration of the serotonin antagonist methysergide. Inhibitory effects from NRM, ipsi- and contralateral MRF were reduced or abolished in 2/9, 4/8 and 6/9 cases, respectively, following systemic administration of the noradrenergic antagonist phentolamine. These results confirm and extend previous studies of medullospinal inhibition and the role of monoamines, and are discussed in terms of analgesic mechanisms.

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A comparative study of selective stimulation of raphe nuclei in the cat in inhibiting dorsal horn neuron responses to noxious stimulation

Cited by 13 publications

References 18 publications

Adolescent ethanol drinking promotes hyperalgesia, neuroinflammation and serotonergic deficits in mice that persist into adulthood

Adolescent ethanol drinking promotes hyperalgesia, neuroinflammation and serotonergic deficits in mice that persist into adulthood

Adolescent ethanol drinking promotes hyperalgesia, neuroinflammation and serotonergic deficits in mice that persist into adulthood

Inhibition of the responses of cat dorsal horn neurons to noxious skin heating by stimulation in medial or lateral medullary reticular formation

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