A comparative study of saliva protein analysis

Wolf, Robert O.; Taylor, Leonard L.

doi:10.1016/0003-9969(64)90003-2

Cited by 29 publications

(12 citation statements)

References 3 publications

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“…In order to convert the A205 val ues to protein concentration an extinction coeffi cient E ! mg/ml = 3.1 was used [Scopes, 1974], The method of Lowry et al [1951] is likely to underestimate the protein concentration in saliva [Wolf and Taylor, 1964] and measurement of A205 is likely to overestimate the protein concentration since salivary substances other than protein will contribute to A205. The actual protein concentra tion can therefore be assumed to be in between these values.…”

Section: Assays For Proteinsmentioning

confidence: 99%

Quantitative Study of the Interaction of Salivary Acidic Proline-Rich Proteins with Hydroxyapatite

Bennick¹,

Cannon²

1978

Caries Res

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Using specific antisera to the proline-rich proteins A and C, a quantitative assay has been developed for human acidic proline-rich proteins. By adding varying amounts of hydroxyapatite to a given volume of saliva, the adsorption of acidic proline-rich proteins and total protein to hydroxyapatite has been determined for stimulated and unstimulated parotid and submandibular saliva. The maximum amount of acidic proline-rich proteins adsorbed (ADS_max) varied from 85 to 97% of the total acidic proline-rich proteins present in unadsorbed saliva. ADS_max for total protein varied from 41 to 79%. The amount of hydroxyapatite needed for half maximal adsorption (HA₅₀) was consistently smaller for acidic proline-rich proteins than for total protein and for the secretions from any gland, HA₅₀ was consistently smaller for unstimulated than stimulated saliva. HA₅₀ values from one individual were consistently smaller than the corresponding HA₅₀ values from a second individual. The mean concentration of acidic proline-rich proteins in unadsorbed saliva was 50 ± 26 mg/l00 ml and the mean contribution of acidic proline-rich proteins to hydroxyapatite-adsorbed proteins was 42% ± SD 23 of total adsorbed proteins. On the basis of equilibrium dialysis experiments with purified protein A and C in buffers with pH and ionic strength similar to salivary secretions, it was estimated that the concentration of calcium bound to acidic proline-rich proteins varied from 8 μM in unstimulated submandibular saliva to 41 μM in stimulated submandibular saliva. The concentration of calcium bound to acidic proline-rich proteins therefore apparently depends on the type of salivary secretion.

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Section: Assays For Proteinsmentioning

confidence: 99%

Quantitative Study of the Interaction of Salivary Acidic Proline-Rich Proteins with Hydroxyapatite

Bennick¹,

Cannon²

1978

Caries Res

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“…This is probably due to differences in the mechanisms by which each method determines protein content. The Lowry method is dependent on the presence of aromatic amino acids (Wolf and Taylor, 1964) which are negligible in the proline-rich proteins of rat parotid saliva (Keller et at, 1975;Iversen et at, 1982). In contrast, the Arneberg method employs absorption at 215 nm, a wavelength which determines protein concentration primarily by absorption due to the peptide bond.…”

Section: Discussionmentioning

confidence: 97%

Influence of Thyroxine in the Regulation of Rat Parotid Salivary Protein Composition

Johnson

Kalu

1988

J Dent Res

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This study tested the role of thyroxine in the regulation of the protein composition of rat parotid saliva. Thyro-parathyroidectomy was performed on two groups of rats, one of which subsequently received thyroxine replacement; the third group was sham-operated. Parotid saliva was collected on the eighth day after surgery, with pilocarpine and isoproterenol used as a secretory stimulus. The volume of saliva collected in 30 min from the thyro-parathyroidectomized rats was 55% less than that collected from sham-operated rats. In the thyro-parathyroidectomized rats, the protein concentration as measured by absorption at 215 nm was unaltered, but that measured by the Lowry procedure was 43% higher. Spectrophotometric scans of Coomassie Blue-stained gels following sodium dodecylsulfate polyacrylamide gel electrophoresis of the secreted proteins showed an 18% reduction in the proportion of protein attributable to amylase and a 43% reduction in proportion of acidic and basic proline-rich proteins following thyro-parathyroidectomy; deoxyribonuclease and two other major secretory proteins (Fraction I and Fraction V) were increased (38%, 20%, and 46%, respectively). These changes in flow rate, protein concentration by the Lowry assay, and protein composition were prevented by treatment of thyro-parathyroidectomized rats with thyroxine replacement and are in opposition to those changes we reported earlier for hyperthyroid rats. The results indicate that the flow of saliva as well as the synthesis of the various salivary proteins are influenced by thyroxine.

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“…[2361] Only a small number of medical testing labs have so far developed the technology to assay such a lower concentration of biologically active molecules like proteins,[255162] RNA,[2163] and DNA. [64] The technology however is improving all the time.…”

Section: Incredible Insights Into Health and Disease Are Offered Thromentioning

confidence: 99%

“…[54] Several tests are in the pipeline for uses ranging from pregnancy testing[717879] to the detection of chemicals such as alcohol[9] and other drugs. [81880] One of the hurdles in developing new tests is a lack of understanding of the human proteome,[54] or the study of large sets of proteins,[256272] particularly those that can serve as biomarkers[8182] for the presence of disease. [1656683]…”

Section: What's Next? Saliva Testing Offers a Potential Substitute Tomentioning

confidence: 99%

“…[62728589] Identifying all of the serum proteins present in saliva could take many more years. With advances in instrumentation, it is predicted that the number of serum proteins identified in saliva will increase significantly, although it will probably never match the number of serum proteins found in blood,[90] mainly because serum proteins[72] are only a tiny part of saliva, described as a dilute, watery solution containing electrolytes, minerals, buffers, and proteins.…”

Section: What's Next? Saliva Testing Offers a Potential Substitute Tomentioning

confidence: 99%

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Science behind human saliva

Tiwari

2011

J Nat Sc Biol Med

138

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Saliva is a complex fluid, which influences oral health through specific and nonspecific physical and chemical properties. The importance of saliva in our everyday activities and the medicinal properties it possesses are often taken for granted. However, when disruptions in the quality or quantity of saliva do occur in an individual, it is likely that he or she will experience detrimental effects on oral and systemic health. Often head and neck radiotherapy has serious and detrimental side effects on the oral cavity including the loss of salivary gland function and a persistent complaint of a dry mouth (xerostomia). Thus, saliva has a myriad of beneficial functions that are essential to our well-being. Although saliva has been extensively investigated as a medium, few laboratories have studied saliva in the context of its role in maintaining oral and general health.

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A comparative study of saliva protein analysis

Cited by 29 publications

References 3 publications

Quantitative Study of the Interaction of Salivary Acidic Proline-Rich Proteins with Hydroxyapatite

Quantitative Study of the Interaction of Salivary Acidic Proline-Rich Proteins with Hydroxyapatite

Influence of Thyroxine in the Regulation of Rat Parotid Salivary Protein Composition

Science behind human saliva

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