A Comparative Study of Protein Profiling by Proteomic Analysis in Camptothecin-Resistant PC3 and Camptothecin-Sensitive LNCaP Human Prostate Cancer Cells

Hasegawa, Naoto; Mizutani, Kosuke; Suzuki, Tohru; Deguchi, Takashi; Nozawa, Yoshinori

doi:10.1159/000096340

Cited by 14 publications

(11 citation statements)

References 44 publications

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“…Our proteomic analysis revealed a panel of proteins increased in a gemcitabine-resistant NSCLC cell line compared with its gemcitabine-sensitive control. Among the differently regulated proteins, six proteins (60 kDa heat shock protein, alpha enolase, heat shock cognate 71 kDa protein, protein disulfide isomerase, sorcin, annexin A1) have been found to be related to chemotherapy resistance in previous proteomic studies [13][14][15][16][17][18], and our findings are consistent with most of the previous works (details in Table 1). Furthermore, our study also identified eight gemcitabine-resistance-associated proteins which have not been reported in previous proteomic studies on chemotherapy resistance (details in Table 1).…”

Section: Discussionsupporting

confidence: 92%

Comparative proteomic profiling identified sorcin being associated with gemcitabine resistance in non-small cell lung cancer

Yang

Liu

et al. 2009

Med Oncol

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Although gemcitabine-based chemotherapy is one of the more effective chemotherapy regimens against NSCLC, there are still many patients who do not benefit from this therapy. The mechanism of initial or acquired resistance to gemcitabine chemotherapy remains unknown. In this study, we investigated the protein profiling in gemcitabine-resistant and gemcitabine-sensitive NSCLC cell lines by a proteomic technology in order to identify novel gemcitabine resistance associated biomarkers for NSCLC patients. The proteomic profiling of NSCLC cell line H460 and its gemcitabine-resistant subline H460/GEM were compared by an isotope-coded affinity tag technology and tandem mass spectrometry. We further validated the expression of sorcin, a gemcitabine-resistance-related protein identified by proteomics, in 62 NSCLC specimens by immunohistochemistry. Fourteen gemcitabine resistance-related proteins were identified including nine up-regulated proteins and five down-regulated proteins. Immunohistochemical results demonstrated that sorcin staining was seen in 66.1% of NSCLC tumors, and sorcin overexpression was associated with gemcitabine resistance and a poor prognosis in NSCLC patients. In conclusion, sorcin might play an important role in the resistance to gemcitabine, and it could also be a novel candidate biomarker for predicting the response of NSCLC patients to gemcitabine treatment.

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Section: Discussionsupporting

confidence: 92%

Comparative proteomic profiling identified sorcin being associated with gemcitabine resistance in non-small cell lung cancer

Yang

Liu

et al. 2009

Med Oncol

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“…These cell lines differ in their phenotypes and malignancy levels and reflect the androgenindependent progression of PCa: more interestingly, they show dissimilar expression profiles of ANXA1 protein. 16 Thus, we have analyzed the effects of shortand long-term hypoxic conditions on ANXA1 expression and on the metastatic-associated functional behaviors of transiently ANXA1 knock down and overexpression in PCa cells.…”

Section: Introductionmentioning

confidence: 99%

Hypoxia regulates ANXA1 expression to support prostate cancer cell invasion and aggressiveness

Bizzarro

Belvedere

Migliaro

et al. 2016

Cell Adhesion & Migration

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Annexin A1 (ANXA1) is a Ca 2C -binding protein overexpressed in the invasive stages of prostate cancer (PCa) development; however, its role in this tumor metastatization is largely unknown. Moreover, hypoxic conditions in solid tumors have been related to poor prognosis in PCa patients. We have previously demonstrated that ANXA1 is implicated in the acquisition of chemo-resistant features in DU145 PCa cells conferring them a mesenchymal/metastatic phenotype. In this study, we have investigated the mechanisms by which ANXA1 regulates metastatic behavior in LNCaP, DU145 and PC3 cells exposed to hypoxia. ANXA1 was differentially expressed by PCa cell lines in normoxia whereas hypoxic stimuli resulted in a significant increase of protein expression. Additionally, in low oxygen conditions ANXA1 was extensively secreted out-side the cells where its binding to formyl peptide receptors (FPRs) induced cell invasion. Loss and gain of function experiments performed by using the RNA interfering siANXA1 and an ANXA1 over-expressing plasmid (MF-ANXA1), also confirmed the leading role of the protein in modulating LNCaP, DU145 and PC3 cell invasiveness. Finally, ANXA1 played a crucial role in the regulation of cytoskeletal dynamics underlying metastatization process, such as the loss of adhesion molecules and the occurrence of the epithelial to mesenchymal transition (EMT). ANXA1 expression increased inversely to epithelial markers such as E-cadherin and cytokeratins 8 and 18 (CKs) and proportionally to mesenchymal ones such as vimentin, ezrin and moesin. Our results indicated that ANXA1 may be a key mediator of hypoxia-related metastasis-associated processes in PCa.

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“…ANX1 exerts different effects in different cancers. Studies have indicated ANX1 was associated with the occurrence of prostate cancer (12) and pancreatic cancer (13). Paweletz et al (14) found that the loss or decreased expression of ANX1 was related to the early events in esophageal cancer.…”

Section: Discussionmentioning

confidence: 99%

Differentially Expressed Proteins between Esophageal Squamous Cell Carcinoma and Adjacent Normal Esophageal Tissue

Xiong¹,

Huang²,

Cai³

et al. 2012

Journal of Medical Biochemistry

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Summary: Proteomics was employed to identify the differentially expressed proteins between esophageal squamous cell carcinoma (ESCC) and adjacent normal esophageal tissues. ESCC tissues and adjacent normal tissues were obtained from 10 patients with ESCC and the proteins were extracted and subjected to two-dimensional gel electrophoresis (2-DE). The differentially expressed proteins were identified after image analysis, and matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) was used to confirm these proteins. Immunohistochemistry was then performed to detect the expressions of HSP27 and ANX1 in ESCC tissues and adjacent normal tissues. A total of 6 differentially expressed proteins were identified by peptide mass fingerprinting, among which SCCA1, KRT4 and ANX1 were down-regulated and TIM1, MnSOD and HSP27 up-regulated in the ESCC. Immuno histochemistry showed HSP27 was highly expressed in the ESCC which, however, had a low expression of ANX1. These findings were consistent with those in proteomics. There were differentially expressed proteins between ESCC and adjacent normal tissues. The investigation of differentially expressed proteins between ESCC and normal esophageal tissue may provide evidence for the molecular pathogenesis of ESCC.Keywords: esophageal squamous cell carcinoma, proteomics, differentially expressed proteins, immunohistochemistry Kratak sadr`aj: Uz pomo} proteomike, identifikovali smo proteine ~ija se ekspresija razlikuje u planocelularnom karcinomu jednjaka (PKJ) i susednim zdravim tkivima jednjaka. Uzorci tkiva PKJ i susednih normalnih tkiva uzeti su od 10 pacijenata sa PKJ a proteini su ekstrahovani i podvrgnuti dvodimenzionalnoj elektroforezi na gelu (2-DE). Proteini sa razli~itom ekspresijom su identifikovani posle analize slika, dok je za njihovo potvr|ivanje upotrebljena tehnika MALDI-TOF-MS (matrix-assisted laser desorption ionisation time-offlight mass spectrometry). Potom su obavljene imunohistohemijske analize kako bi se utvrdile ekspresije HSP27 i ANX1 u tkivima PKJ i susednim normalnim tkivima. Pomo}u tehnike peptide mass fingerprintinga identifikovano je ukupno {est proteina sa razli~itim ekspresijama, od kojih je ekspre sija SCCA1, KRT4 i ANX1 bila sni`ena, a TIM1, MnSOD i HSP21 povi{ena u PKJ. Imunohistohemijsko ispitivanje je pokazalo da je HSP27 veoma izra`en u PKJ, u kom je, me|utim, utvr|ena niska ekspresija ANX1. Ovi rezultati se sla`u sa onima dobijenim pomo}u proteomike. Utvr|eno je da postoje proteini sa razli~itom ekspresijom u PKJ i susednim normalnim tkivima. Istra`ivanje razli~ite ekspresije proteina u PKJ i normalnom tkivu jednjaka mo`e doneti nove dokaze u vezi s molekulskom patogenezom PKJ.Klju~ne re~i: planocelularni karcinom jednjaka, proteomika, proteini s razli~itom ekspresijom, imunohistohemija

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A Comparative Study of Protein Profiling by Proteomic Analysis in Camptothecin-Resistant PC3 and Camptothecin-Sensitive LNCaP Human Prostate Cancer Cells

Cited by 14 publications

References 44 publications

Comparative proteomic profiling identified sorcin being associated with gemcitabine resistance in non-small cell lung cancer

Comparative proteomic profiling identified sorcin being associated with gemcitabine resistance in non-small cell lung cancer

Hypoxia regulates ANXA1 expression to support prostate cancer cell invasion and aggressiveness

Differentially Expressed Proteins between Esophageal Squamous Cell Carcinoma and Adjacent Normal Esophageal Tissue

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