A comparative study of progressive versus successive spectrophotometric resolution techniques applied for pharmaceutical ternary mixtures

Computation of geometric representation of novel spectrophotometric methods used for the analysis of minor components in pharmaceutical preparations

“…This method is considered to be a modification of the derivative compensation ratio technique [14][15][16], but instead of using the amplitude of derivative spectra, the area under the curve was used. It could be applied when the absorption spectra of two components (X) and (Y) overlap to a large extent or when analysis of a minor component (X) in the presence of a major component (Y) represents a problem.…”

“…Tetryzoline HCl (TZH) is combined together with antibiotics, corticosteroids and anti-histamines to formulate anti-infective eye preparations to treat acute and sub-acute conjunctivitis, keratitis and corneal ulcers [9]. Different analytical techniques were reported for TZH determination in pharmaceutical preparations and in biological fluids such as: colorimetric determination [10], spectrophotometry [11][12][13][14], high performance thin layer chromatographic method (HPTLC) [15], high performance liquid chromatographic method (HPLC) [16][17][18][19][20][21] and gas chromatography [22]. Two ophthalmic combinations were selected for the analysis of the minor component TZH; the first one is mixture (A): Ofloxacin (OFX), prednisolone acetate (PA) and TZH in the ratio of (7.5:5:1) respectively, while the second one is mixture (B): sodium cromoglicate (SCG) and TZH in the ratio of (80:1) respectively.…”

Three New Methods for Resolving Ternary Mixture with Overlapping Spectra: Comparative Study

“…The developed area under the curve ("AUC"), modified absorption factor method (MAFM) and modified amplitude center method (MACM) are firstly introduced for resolving ternary mixture of overlapping spectra. The newly introduced analytical methods are a modification of the recently developed spectrophotometric methods, [11][12][13][14][15][16][17][18][19][20][21][22] while the same author published a PLS chemometric method for analysis of the same ternary mixture since 2012.…”

“…MACM This newly introduced method is a combination between the previously used amplitude center (ACM), 20) and ratio difference (RD), 21,22) spectrophotometric methods. It depends on simultaneous quantitation of the three studied components using a single divisor [the same as ACM] through two steps; amplitude calculation via ratio difference and amplitude subtraction depending on using the principle of RD method [advantage over ACM].…”

A comparative study of novel spectrophotometric resolution techniques applied for pharmaceutical mixtures with partially or severely overlapped spectra

“…In this work, two recently introduced resolution techniques were applied namely successive [21][22][23] and progressive resolution techniques [24][25][26]. The resolution of spectra of the cited drugs can be achieved via those techniques based on the use of smart original mathematical techniques where successive techniques consist of several consecutive steps utilizing ratio of zero order absorption and/or derivative spectra while, progressive resolution consists of single manipulation step.…”