A Comparative Study of Phytochemical Screening and DPPH Radical Scavenging Activity of Ficus carica Linn. Leaves Extracts

Abstract

This study focused on two extraction solvents and the examination of antioxidant with the most reliable antioxidant activity test. Furthermore, this study aimed to compare the phytochemical components and antioxidant activity of two Ficus carica Linn. extracts using the local (Indonesian) Ficus carica Linn. plant as the sample. The extraction was conducted by maceration technique using two organic solvents, namely methanol, and ethanol. The phytochemical screening of these extracts was conducted on several metabolite classes, namely alkaloids, flavonoids, steroids, tannins, glycosides, and saponins. In addition, the total phenolic and flavonoid compounds were determined with gallic acid and quercetin, respectively for standards. The antioxidant activity assay was performed using the 2,2-diphenyl-1-picryl hydrazyl-hydrate (DPPH) method with ascorbic acid as the comparative standard. The phytochemical screening of the extracts showed positive results for all tested metabolite classes, however, the methanol extract showed a negative result to alkaloids compounds. The total phenolic and flavonoid compounds were 33.93 ± 0.31, 40.76 ± 0.23 mg GAE/g and 26.28 ± 0.20, 24.35 ± 0.31 mg QE/g for both ethanol and methanol extracts, respectively. Furthermore, the assay showed that both extracts had strong antioxidant activity, however, the methanol extract is slightly higher. The result also showed that methanol extract of F. carica Linn. leaves has fewer phytochemical compounds but demonstrates higher antioxidant activity compared to the ethanolic extract.

Oxidative stability of avocado oil blended with different levels of ultrasonic extract of Chlorella vulgaris under different temperatures

“…Similarly, Odontonema strictum's active fraction showed varying RSAs for different compounds, suggesting a concentration-dependent response (Luhata et al , 2023). In addition, C. vulgaris extracts showed enhanced antioxidant activity with increasing concentration, with 80% ethanol yielding the highest scavenging activity, aligning with the concentration-dependent trend observed in other studies (Reveny et al , 2023). The RSA of C. vulgaris is influenced by the polarity of the solvent due to the solubility of phenolic compounds (Pohanka, 2016).…”

Chemical Elucidation, Microbial Growth and Free Radical Inhibitory Effects of Dennettia tripetala Fruit: In vitro and In vivo Model Experiments

“…A reference control, vitamin C displayed a high activity than the two extracts with IC50 values, 0.007 µg/mL for DPPH and 0.005 µg/mL for ABTS, respectively. Free radicals when produced in an uncontrolled amount have been shown with unusual alteration and specifically targeted at the DNA base sequence and important biomolecules in human 59 Inhibition of DPPH and ABTS radical solution by D. tripetela crude extract are thought to be mediated by certain endogenous chemicals such as phenolics and flavonoids, 60 known for the scavenging activities which has earlier been reported by. 61,62 It is most likely that the plant act basically as an in vitro electron donating agent aimed at averting the proliferation of the radicals emanating from the synthetic free radicals (DPPH and ABTS) employed.…”