A comparative study of osteopontin expression, Ki67 index and prognosis in squamous cell carcinoma and cysts of the oral cavity

Abstract

Background: Recent studies have shown a strong relationship between the expression of osteopontin and oral carcinogenesis. Osteopontin (OPN) has been shown to play a major role in regulating the aggressiveness of cancer cells and promote tumor growth. Odontogenic cysts are an essential aspect of oral and maxillofacial pathology. They are relatively frequent lesions with different clinical behavior. Some of them may have a proliferative pattern of growth and neoplastic nature. Evaluation of osteopontin expression with Ki-67 index may help examine clinical behavior and recurrence of oral squamous cell cancer and radicular cyst patients.Methods: A total of 44 oral cavity cancer cases and 21 cysts samples were analyzed by immunohistochemical staining. Data used for analysis were derived from medical records. The following information was obtained from all patients' medical records: survival, age, sex, lymph node status, tumor size, and location, as well as grade and histologic type of tumor. Expression status of OPN and Ki-67 was statistically assessed.Results: Our data demonstrated that for summary immunoreactive scores of OPN and Ki-67 expressions in OSCC vs. RC patients statistical significance was found for both markers' between OSCC and RC groups. Moreover, osteopontin is significantly higher expressed in larger OSCC tumors. Conclusions:In conclusion, the role of OPN expression both in oral squamous cancer cells and radicular cyst and possible correlation with demographic and clinicopathological features remain undetermined in some aspects, further high-powered studies to develop a more standardized assessment of Ki-67 and osteopontin expression in OSCC and are needed.

Correlation between Amelogenin and Osteopontin Expression in Ameloblastomas and Odontogenic Cysts: An Immunohistochemical Study

“…In full agreement with our findings, Kechik and Siar [31] results showed under-expression and indistinctive distribution patterns of OPN among all studied samples of three types of cysts RCs, DCs and OKCs. Similarly, our observations of OPN concurred with the findings of existing studies of Salehinejad et al [32] and Woźniak et al [33] who reported absent or weak expression of this antibody in RCs and DCs. This was clarified by that OPN could be well expressed in non-malignant tissues because of its multiple physiological activities.…”

Outcome of Patients With Head and Neck Squamous Cell Carcinoma Can be Predicted by Expression of eIF4E and Osteopontin in Free Surgical Margins

“…This finding was consistent with most of the reviewed authors. 1 However, Ingale et al 29 found a significant higher expression of Osteopontin in HNSCC patients above 51 years of age. Osteopontin expression was significantly high in ulcerative pattern, lymphovasular invasion and extracapscular extension.…”

“…Consequently, the primary role of Osteopontin immunoreactivity in tumor progression and an increased risk of metastasis have already been considered. 1 Luo et al 36 demonstrated a positive correlation between Osteopontin immunoreaction and unfavorable overall survival rates. These results were in agreement with ours as we observed significant shorter DFS in high expression of Osteopontin (mean DFS 18.7 ± 1.6 mo vs. 35 ± 0.6 in the negative expression group) and inferior overall survival among the same group as well.…”

“…The cases were divided according to the resulting score into negative: 0, low: 1 to 3, high: 4 to 6. 1 Regarding the immunohistochemical evaluation of eIF4E staining extent, a score of "0" for absent staining, score "1" was assigned to staining in 1% to 10% of the malignant cells, a score of "2" was assigned to 11% to 50%, a score of "3" was assigned to 51-80%, and a score of "4" was assigned to 81% to 100%. According to staining intensity, it was calculated as follows: score 0, no staining; score 1, weak staining; score 2, moderate staining; and score 3, strong staining.…”

“…It is considered the fifth most common cancer that originates in different anatomical sites, including the oral cavity, the oropharynx, the larynx, and the hypopharynx. The 5-year survival rate for this tumor has not increased over recent decades, despite recent therapeutic modalities, with 80% for early identified lesions and 30% to 50% for advanced tumors 1…”