A comparative study of odontogenic keratocyst and orthokeratinized odontogenic cyst using Ki67 and α smooth muscle actin

Dandena, Vinay Kumar; Thimmaiah, Satish Yadava; Kiresur, Mohammad Asif; Hunsigi, Prahlad; Roy, Satyajeet; Rashmi, M

doi:10.4103/jomfp.jomfp_71_17

Cited by 12 publications

(5 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thereby controlling lesion growth and vascular support through angiogenesis ( 10 , 16 ). Unfortunately, few studies on the presence of MF in odontogenic cysts are available ( 2 , 5 , 10 , 18 - 23 ) and only four address inflammatory odontogenic lesions ( 16 - 17 , 24 - 25 ).…”

Section: Discussionmentioning

confidence: 99%

Myofibroblasts and increased angiogenesis contribute to periapical cystic injury containment and repair

de-Freitas

de-França²,

Gordón-Núñez³

et al. 2020

Med Oral

View full text Add to dashboard Cite

Background Myofibroblasts (MF) and angiogenesis are important factors in the development and expansion of cystic lesions, where these cells secrete growth factors and proteases, stimulating angiogenesis, matrix deposition and cell migration, affecting the growth of these periapicopathies. The present study aimed to evaluate the immunohistochemical expression of CD34 and α-SMA in radicular cysts (RC) and residual radicular cysts (RRC), with the purpose of contributing to a better understanding of the expansion and progression of these periapical lesions. Material and Methods The present study os a descriptive, quantitative and comparative analysis of positive CD34 and α-SMA immunohistochemical expressions in 30 RC and 30 RRC specimens. α-SMA expression was evaluated in the fibrous capsule of the lesions, at 100x magnification below the epithelial lining. A total of 10 higher immunostaining fields were selected and subsequently, positive cells were quantified at 400x magnification, averaged per field. Regarding the angiogenic index, immuno-labeled microvessel counts for the anti-CD34 antibody were performed in 10 fields at 200x magnification. Results Statistically significant differences regarding α-SMA immunostaining were observed ( p = 0.035), as well as a correlation between α-SMA versus CD34 ( p = 0.004) in RRC. However, the angiogenic index obtained by immunostaining for CD34 indicated no statistical difference between lesions. Intense inflammatory infiltrates were predominant in RC, while mild and moderate degrees were more commonly observed in RRC ( p <0.001). Intense inflammatory infiltrates were also more often noted in larger RRC ( p = 0.041). Inflammatory infiltrates showed no significant correlation with α-SMA and CD34 immunostaining. Conclusions The results indicate that the significant correlation found between the presence of MF and the angiogenic index are related to the repair process in RRC. Key words: Myofibroblasts, angiogenesis, inflammatory odontogenic cysts.

show abstract

Section: Discussionmentioning

confidence: 99%

Myofibroblasts and increased angiogenesis contribute to periapical cystic injury containment and repair

de-Freitas

de-França²,

Gordón-Núñez³

et al. 2020

Med Oral

View full text Add to dashboard Cite

show abstract

“…MFs are differentiated broblasts that express α-SMA and have intermediate characteristics between conventional broblasts and smooth muscle cells [30] being able to remodel and degrade the extracellular matrix by secreting matrix metalloproteinases, driving the invasive growth of lesions odontogenic cyst [31]. The few studies that investigated the presence of MFs in odontogenic lesions suggest that the increase in the number of these cells contributes to the progression of the lesion and more aggressive biological behavior [13,17,21,24,25,32,33]. Anusai et al [10] reported that epithelial and stromal cell interactions are fundamental in controlling the growth and clinical behavior of odontogenic cystic lesions and that MFs are seen in greater numbers in OKC than in DC.…”

Section: Discussionmentioning

confidence: 99%

Stromal Myofibroblasts in Correlation With Inflammation and Impacted Tooth in Odontogenic Cysts: An Immunohistochemical Comparative Study

Martins

Barbeiro

et al. 2023

Preprint

View full text Add to dashboard Cite

Aim Stromal myofibroblasts may act favoring growth and progression of odontogenic lesions, notably odontogenic keratocyst (OKC). While little explored the associated inflammation, it has been shown that OKC associated with an impacted tooth has a tendency toward rapid growth. Whether this finding is associated with the differential expression of myofibroblast immunomarkers, is unknown. Materials and methods Thirteen cases of each, pericoronal follicle (PF), inflamed dentigerous cyst (iDC), noninflamed DC (nDC), OKC associated with (OKC-A) or not associated (OKC-N) with impacted tooth, were evaluated through vimentin, α-SMA, calponin and h-caldesmon immunomarkers The data obtained were analyzed using the Shapiro-Wilk, Kruskal-Wallis and Dwass-Steel-Critchlow-Fligner Multiple Comparisons tests, with p < 0.05 being considered statistically significant. Results All cases were vimentin positive, with few cases exhibiting mild expression. All PFs were α-SMA negative. OKCs showed significant higher expression of α-SMA than DCs (p < 0.05). With similar expression between DC groups, the α-SMA expression was higher in OKC-A than OKC-N (p = 0.882). Also, the mean age of OKC-A was significantly lower than OKC-N patients. Only 4 cases (1 iDC, 3 OKC-N) showed mild expression of calponin. All cases were h-caldesmon negative. Conclusions Our results suggest lack of myofibroblastic differentiation in PFs. Inflammation does not seem to influence the myofibroblast population in DCs. Although not significant, a higher expression of α-SMA can explain the clinicopathological features of OKC-A patients. Calponin seems not to be a reliable marker for myofibroblasts in these cases. Clinical relevance Lack of myofibroblastic differentiation seems to be consistent with the nature and function of PF. OKC (still, OKC-A than OKC-N) presents higher α-SMA expression than DC, which can help to explain its distinctive growth potentials.

show abstract

“…3 While the latter is an aggressive lesion that tends to cause massive areas of destruction by proliferating in between the marrow spaces in an anteroposterior direction, the former is a relatively docile one. 5 The present case report describes an unusual case of extensive OOC that involved the majority of the angle and ramus of the mandible.…”

Section: Introductionmentioning

confidence: 86%

An unusually extensive orthokeratinized odontogenic cyst: A case report

Sachdev,

Adhane,

Sardar

et al. 2023

JDPO

View full text Add to dashboard Cite

While odontogenic keratocysts (OKC) are fairly common and well-known, orthokeratinized odontogenic cysts (OOCs) are quite rare accounting for less than 1% of odontogenic cysts. The entity is characterized by the presence of a predominant orthokeratinized lining. OOC is much less aggressive as compared to OKC and simple enucleation is discerned as adequate treatment with minimal chances of recurrence. The present case report describes an extensive OOC that involved most of the angle and ramus of the mandible in a 25-year-old female.

show abstract

A comparative study of odontogenic keratocyst and orthokeratinized odontogenic cyst using Ki67 and α smooth muscle actin

Cited by 12 publications

References 10 publications

Myofibroblasts and increased angiogenesis contribute to periapical cystic injury containment and repair

Myofibroblasts and increased angiogenesis contribute to periapical cystic injury containment and repair

Stromal Myofibroblasts in Correlation With Inflammation and Impacted Tooth in Odontogenic Cysts: An Immunohistochemical Comparative Study

An unusually extensive orthokeratinized odontogenic cyst: A case report

Contact Info

Product

Resources

About