A Comparative Study of Newly Developed HPLC-DAD and UHPLC-UV Assays for the Determination of Posaconazole in Bulk Powder and Suspension Dosage Form

Abstract

Objective. To develop and compare HPLC-DAD and UHPLC-UV assays for the quantitation of posaconazole in bulk powder and suspension dosage form. Methods. Posaconazole linearity range was 5–50 μg/mL for both assays. For HPLC-DAD assay, samples were injected through Zorbax SB-C18 (4.6 × 250 mm, 5 μm) column. The gradient elution composed of the mobile phase acetonitrile: 15 mM potassium dihydrogen orthophosphate (30 : 70 to 80 : 20, linear over 7 minutes) pumped at 1.5 mL/min. For UHPLC-UV assay, samples were injected through Kinetex-C18 (2.1 × 50 mm, 1.3 μm) column. The mobile phase composed of acetonitrile: 15 mM potassium dihydrogen orthophosphate (45 : 55) pumped isocratically at 0.4 mL/min. Detection wavelength was 262 nm in both methods. Results. The run time was 11 and 3 minutes for HPLC-DAD and UHPLC-UV assays, respectively. Both assays were linear (r 2 > 0.999) with CV% and % error of the mean <3%. Limits of detection and quantitation were 0.82 and 2.73 μg/mL for HPLC-DAD and 1.04 and 3.16 μg/mL for UHPLC-UV, respectively. The methods quantitated PSZ in suspension dosage form with no observable interferences. Conclusions. Both assays were proven sensitive and selective according to ICH guidelines. UHPLC-UV assay exhibited some economic and chromatographic separation superiority.

A Disposable Carbon Nanotubes‐screen Printed Electrode (CNTs‐SPE) for Determination of the Antifungal Agent Posaconazole in Biological Samples

“…To decrease the volume of blood sampling, SPE was used for the determination of the drug in human plasma and DBS after sample pretreatment. The results obtained by the proposed method were compared with those obtained from the reported methods ,, respectively according to t‐test and F‐test (Table ).…”

“…It is a selective inhibitor of 14‐α‐demethylase enzyme, leading to accumulation of 14‐α‐methylated sterols that induce membrane leakiness, unbalanced chitin synthesis, cell disorganization, and death of the pathogens. POS is mostly used in case of fungal infection linked with immune compromised patients …”

High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Posaconazole and Vincristine in Rat Plasma

“…During assay optimization, the authors utilized the exact chromatographic conditions that they recently published for the HPLC determination of PSZ in bulk form and suspension dosage form [ 29 ]. However, HC-C18 instead of Zorbax SB-C18 column was utilized and HC-C18 guard column was added.…”

Occurrence of selected estrogenic compounds and estrogenic activity in surface water and sediment of Langat River (Malaysia)

“…Instrument analysis of steroid estrogens was performed by ultra-performance liquid chromatography (Perkin Elmer FX15) electrospray ionizationmass spectrometry coupled to a QTrap function mass spectrometer (model 3200; AB Sciex). Ultra-high performance liquid chromatography shortens analysis time and sample volume, enhancement in speed and solvent consumption, can operate at high system back-pressures with mobile phase running at upper velocities as compared to h i g h p e r f o r m a n c e l i q u i d c h r o m a t o g r a p h y (Nováková et al 2006;Hamdy and Belal 2014). U l t r a-p e r f o r m an c e li q ui d c hr o m a t o g r ap h y electrospray ionization-mass spectrometry also offers more advantages in quantifying steroid estrogen at low concentrations, as most of the detections of steroid estrogens in sediment is mainly performed using gas chromatography-mass spectrometry, as indicated by the reviews conducted by Aris et al (2014), Kuster et al (2004), and Ternes et al (2002).…”