A comparative study of MTT and WST-1 assays in cytotoxicity analysis

Sarı, Ceren

doi:10.14744/hnhj.2019.16443

Cited by 9 publications

(8 citation statements)

References 21 publications

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“…The basic mechanism of the reaction is that WST-1 combines with mitochondrial succinate tetrazolium reductase to produce a water-soluble formazan dye. 19 In our research, treatment with Eupatorin substantially impeded PA-1 cell proliferation. The experiment revealed the Eupatorin 's IC 50 concentration.…”

Section: Discussionmentioning

confidence: 48%

In vitro and in silico Investigations on the Anti-cancer Efficacy of Eupatorin, a Polymethoxy Flavone against Ovarian Cancer PA-1 Cell Line

Khamjan,

Jerah,

Habibullah

et al. 2024

Ind. J. Pharm. Edu. Res

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Background: Ovarian cancer is a prominent contributor to cancer-related death among women residing in developed countries. The most of instances are discovered when the cancer has already developed, which results in dismal consequences. Surgery, chemotherapy, and radiation were the top priorities for first-line treatment in clinics. Traditional cancer treatments have a substantial risk of toxicity and cancer recurrence in females. Plant extracts can be utilized as an alternative to traditional chemotherapeutic drugs to solve these issues. According to recent research, plant extracts show anti-tumor, anti-cancer, and anti-proliferative effects on human tumor cell lines that have been cultivated, as well as an antiangiogenic impact. Aim: Eupatorin is a class of flavonoids isolated from various medicinal plants with various potent biological properties including anti-cancer, anti-inflammatory, and vasorelaxant actions. Materials and Methods: In the current investigation, the potential of Eupatorin as an anti-cancer agent for ovarian cancer was evaluated. In this study was determination of cytotoxicity using WST-1 assay, LDH release assay, and apoptotic cell death was detected through AO/EB dual staining, estimation of pro-apoptotic markers using ELISA method and in silico analysis. Results: The viability of PA-1 cells was reduced with an increased dosage of Eupatorin. The LDH level was increased with increased concentration of Eupatorin. The apoptosis markers levels were also increased when PA-1 cells were exposed to Eupatorin indicating apoptosis of cancer cells. The results showed that apoptosis was induced by Eupatorin in PA-1 cells by triggering the caspase pathway. In addition, the in silico experiment was done to examine the binding efficacy of Eupatorin with VEGF-A/ VEGFR and found that Eupatorin can bind more persistently to VEGF-A/VEGFR than apratoxin thereby preventing angiogenesis. Conclusion: All these results suggest that Eupatorin can be used as a potent anti-cancer drug for ovarian cancer.

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Section: Discussionmentioning

confidence: 48%

In vitro and in silico Investigations on the Anti-cancer Efficacy of Eupatorin, a Polymethoxy Flavone against Ovarian Cancer PA-1 Cell Line

Khamjan,

Jerah,

Habibullah

et al. 2024

Ind. J. Pharm. Edu. Res

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“…The assay measured the transformation of the tetrazolium salt WST-1 to formazan by cellular dehydrogenases. The formazan generated was dark yellow, measured at 450nm and was directly correlated to cell number (Sarı, 2019).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Cytotoxicity of Linseed Oil as Bio-Based Plasticiser in Polymethylmethacrylate (PMMA) Denture Base Material

Abdullah Zawawi,

Abdul Ghani,

Raja Awang

et al. 2024

Compend. Oral Sci.

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Objectives: Polymethyl methacrylate (PMMA) is a polymer that is vastly utilised as denture base material. Previously synthetic plasticiser like Dibutyl phthalate was previously used in the PMMA. However, there are reports of ester leaching causing intraoral toxicity. Thus, bringing the awareness about organic plasticiser usage, like linseed oil. This study aims to evaluate the cytotoxicity of linseed oil to ensure its safety to be used as the natural-based plasticiser in PMMA as denture base material. Materials and Methods: The cytotoxicity of linseed oil was evaluated by culturing Human Gingival Fibroblast cells with 1, 3, 5, 7, and 10% linseed oil for 24, 48, and 72 hours. The WST-1 assay was then used to evaluate cell viability, with cell quantification performed using a spectrophotometer at a wavelength range of 420-480nm. An unpaired t-test was used to analyse all parameters' quantitative cell viability data. Results: The WST-1 Assay demonstrated that linseed oil exhibited greater than 95% cell viability at all doses and incubation durations, indicating biocompatibility. At 72 hours of incubation, there are statistically significant differences between the cells incubated in the Linseed Oil as compared to Dimethyl Sulfoxide (DMSO), which acts as a positive control. Conclusions: The study proves that linseed oil is non-cytotoxic with the Human Gingival Fibroblast cells and safe to use when incorporated later in the PMMA as denture base material.

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“…Regarding the cytotoxicity assessment, the results of the tests by direct contact performed in HaCaT cells revealed that the developed paints presented cellular viability values over 70%, the established thresholds according to ISO 10993:5 and LDH releases under 30% [ 23 ]. Besides NRU assay, the standard ISO 10993:5, recommends MTT or XTT assay to evaluate cell viability; however, we performed the analogous assay WST-1, since it evaluates the same parameter and has less interferences and produces more accurate results [ 39 ]. In addition, we realized the LDH assay, which evaluates cell lysis.…”

Section: Discussionmentioning

confidence: 99%

Development and In Vitro Validation of Antibacterial Paints Containing Chloroxylenol and Terpineol

Querido

Paulo²,

Hariharakrishnan³

et al. 2022

Toxics

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The establishment of self-disinfecting surfaces is an important method to avoid surface contamination. Recently, paints with antimicrobial properties have been developed to be applied on different surfaces, avoiding contamination with pathogens. In this work, self-disinfecting paints containing Chloroxylenol (CLX), Terpineol (TRP), and a mixture of both substances were developed. The goal was to evaluate and validate these paints using international standards for eventual commercialization and application in scenarios where surface contamination represents a problem. The paints were challenged with five different bacteria, Gram-positive and Gram-negative, before and after a scrub resistance test, where the long-term efficacy of the paints was evaluated. The antibacterial activity assessment was performed following ISO 22196 and JIS Z2801. In general, the paints showed very promising results, demonstrating their antibacterial activity, before and after scrub resistance test. The paint incorporating the mixture of CLX and TRP (CLX+TRP) stood out by revealing consistent results of antibacterial activity both before and after the scrub resistance test for most of the tested bacteria. The cytotoxicity of the developed paints was assessed in vitro by performing tests by direct contact with a human skin cell line, HaCaT, and testes on extracts with HaCaT and a pulmonary cell line, A549. The methodologies for cytotoxicity assessment were developed based in ISO 10993. For genotoxicity assessment, alkaline comet assay was conducted on both cell lines. The cytotoxicity assessment revealed promising results with the paints, demonstrating values of cellular viability above 70% and values of lactate dehydrogenase (LDH) leakage below 30%. The genotoxic assessment also revealed acceptable values of primary DNA damage for the developed antibacterial paints. In general, the selected methodologies presented good potential to be applied in the validation of both efficacy and safety of the antimicrobial paints, aiming to be applied in real scenarios.

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A comparative study of MTT and WST-1 assays in cytotoxicity analysis

Cited by 9 publications

References 21 publications

In vitro and in silico Investigations on the Anti-cancer Efficacy of Eupatorin, a Polymethoxy Flavone against Ovarian Cancer PA-1 Cell Line

In vitro and in silico Investigations on the Anti-cancer Efficacy of Eupatorin, a Polymethoxy Flavone against Ovarian Cancer PA-1 Cell Line

In Vitro Cytotoxicity of Linseed Oil as Bio-Based Plasticiser in Polymethylmethacrylate (PMMA) Denture Base Material

Development and In Vitro Validation of Antibacterial Paints Containing Chloroxylenol and Terpineol

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