A Comparative Study of Media for the Detection of Streptococci in Water and Sewage

Mallmann, W. L.; Seligmann, Edward B.

doi:10.2105/ajph.40.3.286

Cited by 69 publications

(15 citation statements)

References 4 publications

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“…The concentration tested is similar to that used in the culture media used to select some bacterial groups (e.g. enterococci) that do not have the same cytochrome system in the respiratory chain (Mallman and Seligmann 1950).…”

Section: Discussionmentioning

confidence: 99%

Assessment of biological activity and fate of organic compounds in a reactor for the measurement of biodegradable organic carbon in water

Ribas¹,

Frías²,

Ventura³

et al. 1995

Journal of Applied Bacteriology

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A new, rapid method for the determination of biodegradable dissolved organic carbon (BDOC), especially suited to water industry needs, was recently proposed by the authors. This dynamic method measured the BDOC of circulating water continuously pumped over a biofilm attached to a special support (sinterized porous glass) that fills a system of two glass columns. The BDOC value corresponds to the difference in dissolved organic carbon (DOC) between inflow and outflow water samples. The analytical results are not significantly different from those of other bioassays that use indigenous bacteria, and the total duration of the analysis is less than 3 h. However, a problem common to all the BDOC methods based on attached bacteria is the extent to which the decrease in DOC during the BDOC analysis is due to true biodegradation or to adsorption of organic matter to the reactor. In the present study, a reasonable support is provided for the hypothesis that this decrease, at least in the dynamic method, is predominantly due to microbiological activity. After comparing the support (sinterized porous glass) with a good physical adsorbent (granular activated carbon), the influence of temperature, residual chlorine and sodium azide on the reactor performance was tested, and a sensitivity only attributable to biological activity was observed. Another set of experiments were performed to assess the fate and specific elimination of different organic substances, explicable assuming that biodegradation processes were involved.

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Section: Discussionmentioning

confidence: 99%

Assessment of biological activity and fate of organic compounds in a reactor for the measurement of biodegradable organic carbon in water

Ribas¹,

Frías²,

Ventura³

et al. 1995

Journal of Applied Bacteriology

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“…All cultures have been maintained since isolation in 3% tryptic(ase) soy-l6% glycerol-84% water broth a t -18 C. Upon recall from storage, they were cultivated routinely in the basal medium of Mallmann and Seligmann (15), which was modified to exclude the sodium azide and to include 1 ml of 1.6% alcoholic solution of bromocresol purple (MJ medium) per liter. Inoculations of media were made from young broth cultures upon solid media with loops or needles and into liquid media as single drops from pipettes.…”

Section: Methodsmentioning

confidence: 99%

Unidentified Streptococci from Plants

Mundt

1975

International Journal of Systematic Bacteriology

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The unidentified streptococci from plants are catalase negative, gram positive, usually lanceolate cells occurring in pairs and short chains. More than one-half of the 505 strains investigated conform generally to the description of Streptococcus fueciurn, but many deviate in one or more of Sherman's characteristics, growth on bile-esculin agar, and acidification of milk. A few strains superficially resemble Streptococcus luctis. The strains not termed S. fueciurn-like are heterogeneous in adherence to Sherman's characteristics, growth and pigmentation on tellurite and tetrazolium agars, and the heme peroxidase reaction; 35 fermentation patterns were established from the fermentations of arabinose, raffinose, melezitose, melibiose, mannitol, and sorbitol. The diversity in properties indicates that the cultural reactions do not permit definitive characterization or speciation. It is suggested that the S. fueciurn-like strains have provided the ancestral pool from which the species as now described has become adapted to life within the animal host.The unidentified streptococci of plants and vegetables form a heterogeneous group which is second numerically and in frequency of distribution only to Leuconostoc rnesenteroides (20). Some have been described as being Streptococcus fueciurn-like (21, 22) and sharing characteristics with s. fueculis (6). Splittstoesser et al.(29, 30) and White and White (33) isolated atypical streptococci from frozen vegetables.Atypical streptococci bearing the group D antigen have been isolated from feces of pigs (8, 9), feces and tonsils of pigs (13), canned hkLms (2), and humans (18). A characteristic feature of several studies is the highly variant pattern in the fermentations of selected carbohydrates (2, 9), which appears to be shared by members of S. bovis (16,17,25) and S. luctis (31).The streptococci reported in this paper were isolated from plants and vegetables (20). They are usually lanceolate, but sometimes spherical, catalase-negative, homofermentative bacteria occurring in pairs and in short chains. They were studied and are reported as two groups based upon segregation a t the time of isolation. One group is termed S. fueciurn-like, and the second group is other streptococci. MATERIALS AND METHODSMaintenance and propagation of cultures. All cultures have been maintained since isolation in 3% tryptic(ase) soy-l6% glycerol-84% water broth a t -18 C. Upon recall from storage, they were cultivated routinely in the basal medium of Mallmann and Seligmann (15), which was modified to exclude the sodium azide and to include 1 ml of 1.6% alcoholic solution of bromocresol purple (MJ medium) per liter. Inoculations of media were made from young broth cultures upon solid media with loops or needles and into liquid media as single drops from pipettes. Colonial characteristics and pigmentation on 2,3,5-triphenyltetrazolium chloride agar ( 1) were obtained by streaking for isolated colonies. The most reliable reactions on heme peroxidase medium (34) were obtained by making short, heavy st...

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“…Obviously, the criteria of Sherman1" for the enterococcus group were not simple, namely, growth at 100 and 450 C, in 6 sources of pollution, rainfall, and turbidity was obtained.…”

Section: Selection Of Mediamentioning

confidence: 99%

A Comparative Study of Media for Detection of Enterococci in Water

Croft¹

1959

Am J Public Health Nations Health

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A Comparative Study of Media for the Detection of Streptococci in Water and Sewage

Cited by 69 publications

References 4 publications

Assessment of biological activity and fate of organic compounds in a reactor for the measurement of biodegradable organic carbon in water

Assessment of biological activity and fate of organic compounds in a reactor for the measurement of biodegradable organic carbon in water

Unidentified Streptococci from Plants

A Comparative Study of Media for Detection of Enterococci in Water

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