A comparative study of laser spray and electrospray

Kudaka, Ichiro; Kojima, Takanori; Saito, Shingo; Hiraoka, Kenzo

doi:10.1002/1097-0231(20000830)14:16<1558::aid-rcm64>3.0.co;2-3

Cited by 24 publications

(36 citation statements)

References 13 publications

(24 reference statements)

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“…Other activation methods, prompt heating of the inlet of the sample solution, for example, might be effective for more quantitative analysis of the dissociation [21]. We are now trying to apply laser spray, developed by Hiraoka et al [22,23], for quantification of binding affinity of c-Myb DBD-dsDNA complexes [24]. This technique might be promising for the quantification of binding affinity of complexes stabilized mainly by hydrogen bonds and/or electrostatic interactions.…”

Section: Characterization Of the Complex Stability By Cidmentioning

confidence: 99%

Evaluation of protein-DNA binding affinity by electrospray ionization mass spectrometry

Akashi

Osawa

Nishimura

2005

J. Am. Soc. Mass Spectrom.

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Binding affinity of complexes between a DNA-binding domain (DBD) of a transcription factor, c-Myb, and several double-stranded DNA (dsDNA) were evaluated by collision-induced dissociation (CID) of the multiply protonated molecules generated by electrospray ionization mass spectrometry (ESI-MS). Complexes of the c-Myb DBD and dsDNA were prepared in solution and analyzed by ESI-MS. Multiply protonated molecules of a high-affinity complex, the c-Myb DBD and dsDNA with a specific sequence, were clearly observed in ESI mass spectrum. Protonated molecules of the complex were quite stable in the gas-phase, and not easily dissociated even if high cone voltage was applied in the first vacuum chamber source when the sample was prepared in 10 mM ammonium acetate. As for the sample prepared in buffer with higher concentration of ammonium acetate, such as 500 mM ammonium acetate, protein-dsDNA complexes could easily be dissociated with an increase in the cone voltage, giving multiply protonated molecules of free c-Myb DBD and some DNA fragments. Systematic CID experiments were carried out on seven complexes between the c-Myb DBD and 22-mer dsDNA with different solution-Kd values in the range of 10 Ϫ9 M to 10 Ϫ7 M. For each complex dissociation curve as a function of cone voltage was plotted, and the cone voltage where 50% of the complex was dissociated (V 50% ) was calculated. Consequently, positive correlation was obtained between V 50% and relative binding free energy change (⌬⌬G) in complex formation in solution. This suggests that ESI-CID experiments can provide quantitative evaluation of the stability of protein-DNA complexes based on proper calibration. (J Am Soc Mass Spectrom 2005, 16, 116 -125)

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Section: Characterization Of the Complex Stability By Cidmentioning

confidence: 99%

Evaluation of protein-DNA binding affinity by electrospray ionization mass spectrometry

Akashi

Osawa

Nishimura

2005

J. Am. Soc. Mass Spectrom.

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“…So far, ESI was the only choice for generation of ions directly from aqueous solutions. In a laser spray [31,32], one of the numerous types of ESI sources, ions are generated using an explosive evaporation of effusing aqueous solution by focusing a 10.6 m radiation from a CW CO 2 laser on a tip of the spraying capillary. This atmospheric method of ionization using a continuous laser results in formation of multiplycharged ions, like in normal ESI.…”

Section: Use Of Liquid Water As a Matrix In Ap Ir-maldi Ion Sourcementioning

confidence: 99%

Desorption/ionization of biomolecules from aqueous solutions at atmospheric pressure using an infrared laser at 3 μm

Laiko

Taranenko

Berkout

et al. 2002

J. Am. Soc. Mass Spectrom.

102

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A new atmospheric pressure (AP) infrared (IR) matrix-assisted laser desorption/ionization (MALDI) ion source was developed and interfaced with a Thermo Finnigan LCQ ion trap mass spectrometer. The source utilized a miniature all-solid-state optical parametric oscillator (OPO)-based IR laser system tunable in the ϭ 1.5-4 m spectral range and a nitrogen ultraviolet (UV) laser ( ϭ 337 nm) for use in comparative studies. The system demonstrated comparable performance at 3 m and 337 nm wavelengths if UV matrices were used. However, AP IR-MALDI using a 3 m wavelength showed good performance with a much broader choice of matrices including glycerol and liquid water. AP IR-MALDI mass spectra of peptides in the mass range up to 2000 Da were obtained directly from aqueous solutions at atmospheric conditions for the first time. A potential use of the new AP IR-MALDI ion source includes direct MS analysis of biological cells and tissues in a normal atmospheric environment as well as on-line coupling of mass spectrometers with liquid separation techniques. (J Am Soc Mass Spectrom 2002, 13, 354 -361)

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“…῎ 73 ῎ general trend observed for the samples of oligopeptides 44) and proteins. The formation of less highly charged ions for laser spray may be due to the fact that the process of charge enrichment by successive uneven droplet fission is less prominent by laser spray than by electrospray.…”

Section: Development Of New Ionization Methods For Gc/ms and Lc/ms Inmentioning

confidence: 96%

“…13 should be noted. As was already pointed out in our previous paper, 44) selective vaporization of solvent takes place by the laser irradiation resulting in the increase of the sample concentration near the meniscus of the sample solution e#using out from the capillary. The increase of the sample concentration leads to the formation of cluster ions.…”

Section: ῍1mentioning

confidence: 99%

“…For example, the mass spectral pattern of EI (m/z (relative intensity)) for n-octane is 43(100), 57 (30) ῌῌ at m/z 114 for n-octane is only 3῎ of the base peak of the C 3 H 7 ῌ ion at m/z 43 for EI. In contrast, the mass spectral pattern of He῏ APPeI was measured to be 114(100), 113(85), 71 (60), 85(46), 57 (44), 43(9). That is, the base peak is the molecular ion (C 8 H 18…”

Section: Summary and Future Prospectmentioning

confidence: 99%

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Development of New Ionization Methods for GC/MS and LC/MS Interfaces

Hiraoka

2005

J. Mass Spectrom. Soc. Jpn.

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New ionization methods for GC/MS and LC/MS interface which have been developed in our laboratory will be described. The atmospheric pressure Penning ionization was developed for GC/MS interface. Its sensitivity was found to be as good as that of the electron impact ionization method. The laser spray developed for LC/MS interface, which can be regarded as the electric-field assisted MALDI, gave about one order of magnitude higher ion detection sensitivity than the conventional electrospray. In addition, laser spray can detect ions with di#erent surface activities more non-selectively than electrospray.

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A comparative study of laser spray and electrospray

Cited by 24 publications

References 13 publications

Evaluation of protein-DNA binding affinity by electrospray ionization mass spectrometry

Evaluation of protein-DNA binding affinity by electrospray ionization mass spectrometry

Desorption/ionization of biomolecules from aqueous solutions at atmospheric pressure using an infrared laser at 3 μm

Development of New Ionization Methods for GC/MS and LC/MS Interfaces

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