A comparative study of glutathione and ascorbate metabolism during germination of Pinus pinea L. seeds

Tommasi, Franca; Paciolla, Costantino; Pinto, Maria Concetta de; Gara, Laura De

doi:10.1093/jxb/52.361.1647

Cited by 49 publications

(53 citation statements)

References 37 publications

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“…Similarly, in dry Pinus pinea seeds GSSG represented 50% of the total glutathione pool in embryos. This was reduced to less than 4% after 24 h of germination as a result of GSSG reduction by GR (Tommasi et al 2001).…”

Section: Ascorbate and Glutathione During Seed Desiccation And Imbibimentioning

confidence: 90%

Desiccation tolerant plants as model systems to study redox regulation of protein thiols

Colville

Kranner

2010

Plant Growth Regul

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While the majority of plants and animals succumb to water loss, desiccation tolerant organisms can lose almost all of their intracellular water and revive upon rehydration. Only about 300 'resurrection' angiosperms and very few animals are desiccation tolerant. By contrast, many bryophytes and most lichens are desiccation tolerant and so are the seeds and pollen grains of most flowering plants. The current literature reveals that the extreme fluctuations in water content experienced by desiccation tolerant organisms are accompanied by equally extreme changes in cellular redox state. Strongly oxidizing conditions upon desiccation can cause irreversible oxidation of free cysteine residues of proteins, which can change protein structure and function, and contribute to protein denaturation. It appears likely that reversible formation of disulphide bonds, in particular through protein glutathionylation, contributes to the set of protection mechanisms that confer desiccation tolerance. Upon rehydration, de-glutathionylation can be catalyzed by glutaredoxins (GRXs) and protein disulphide bonds can be reduced through NADPH-dependent thioredoxins (TRXs). Due to their ability to survive severe oxidative stress, desiccation tolerant plants and seeds are excellent models to study protein redox regulation, which may provide tools for enhancing tolerance to drought and more generally, to oxidative stress, in crops.

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Section: Ascorbate and Glutathione During Seed Desiccation And Imbibimentioning

confidence: 90%

Desiccation tolerant plants as model systems to study redox regulation of protein thiols

Colville

Kranner

2010

Plant Growth Regul

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show abstract

“…Antioxidant enzyme activities were assayed at 4°C on nondenaturing polyacrylamide gels with a Mini-Protein II electrophoresis cell (Bio-Rad, Hercules, CA, USA) according to the following procedures (using the notation of Righetti 1983); CAT isozymes (7.5% T, 3% C; Anderson et al 1995), APX isozymes (10% T, 3% C; Lee and Lee 2000), SOD isozymes (12% T, 3% C; Beauchamp and Fridovich 1971), GR isozymes (7.5% T, 3% C; Anderson et al 1995), and DHAR isozymes (12% T, 5% C; Tommasi et al 2001).…”

Section: Electrophoretic Determination Of Antioxidant Enzyme Activitiesmentioning

confidence: 99%

Cytological and physiological changes in recalcitrant Chinese fan palm (Livistona chinensis) embryos during cryopreservation

et al. 2011

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Cytological and physiological changes during cryopreservation were investigated in Livistona chinensis embryos excised 42 weeks after flowering. Both dehydration and freezing caused numerous cellular ultrastructural alterations. Dehydration seriously impaired plasma membrane integrity, while freezing caused a further increase in electrolyte leakage. Damage to cellular ultrastructure and plasmalemma integrity had an inverse relationship with water content in unfrozen embryos and a positive relationship in frozen embryos. Changes in activity of antioxidant enzymes differed during cryopreservation. Dehydration and freezing had little effect on superoxide dismutase activity, although these treatments greatly reduced embryo viability. Activity of dehydroascorbate reductase (DHAR) and glutathione reductase (GR) changed only slightly during dehydration, but dehydration markedly decreased activity of ascorbate peroxidase (APX) and catalase (CAT). Freezing further decreased APX and GR activity but increased CAT activity in dehydrated samples. A novel DHAR isozyme was induced during the freeze-thaw cycle. Membrane lipid peroxidation was detected in the control embryos, and was promoted by both dehydration and freezing. The malondialdehyde (MDA) content in post-thaw embryos increased by a maximum of 30%. Thus, changes in viability of embryos were closely related to damage to cellular ultrastructure and plasmalemma integrity, but were not directly related to antioxidant activity nor MDA accumulation.

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“…Ascorbate and glutathione contents were determined according to Sgherri et al (2000) and Tommasi et al (2001), respectively. The content of hydrogen peroxide (H 2 O 2 ) was measured by monitoring the A 410 of titaniumperoxide complex following the method of Patterson et al (1984).…”

Section: Rate Of Alternative Electron Flowmentioning

confidence: 99%

Compensatory acclimated mechanisms of photoprotection in a Xa mutant of Lycopersicon esculentum Mill.

Wang¹,

Xia²,

Zhou³

et al. 2008

Photosynt.

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To probe the role of xanthophylls in non-photochemical quenching (NPQ) and the compensatory acclimated photoprotection mechanisms, a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) Xa mutant with deficit in lutein (L) and neoxanthin (N) contents was used. The Xa mutant showed lowered NPQ, an increased degree of de-epoxidation state [(A+Z)/(V+A+Z)], and decreases of photosystem 2 (PS2) antenna size. Although the Xa mutant had a CO 2 assimilation rate similar to that of Ailsa Craig, it exhibited a much larger stomatal conductance (g s ) than Ailsa Craig. Decreased electron flux in PS2 (J PS2 ) for the Xa mutant was associated with electron flux for photorespiratory carbon oxidation (J o ) and alternative electron flux in PS2 (J a ) while electron flux for photosynthetic carbon reduction (J c ) was not different from Ailsa Craig. Moreover, the Xa mutant also exhibited higher activities of antioxidant enzymes, higher contents of ascorbate and glutathione, and lower contents of reactive oxygen species. Hence some compensatory acclimated mechanisms of photoprotection operated properly in the lack of NPQ and xanthophylls.

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A comparative study of glutathione and ascorbate metabolism during germination of Pinus pinea L. seeds

Cited by 49 publications

References 37 publications

Desiccation tolerant plants as model systems to study redox regulation of protein thiols

Desiccation tolerant plants as model systems to study redox regulation of protein thiols

Cytological and physiological changes in recalcitrant Chinese fan palm (Livistona chinensis) embryos during cryopreservation

Compensatory acclimated mechanisms of photoprotection in a Xa mutant of Lycopersicon esculentum Mill.

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