A comparative study of eubacterial communities by PCR-DGGE fingerprints in anoxic and aerobic biotrickling filters used for biogas desulfurization

Abstract

Biological desulfurization has proven to be a process that is technically and economically feasible on using biotrickling filters that can be performed under aerobic and anoxic conditions. However, microbial communities are different mainly due to the use of different final electron acceptors. The analysis of microbial communities in these systems has not been addressed with regard to the anoxic process. The aim of the work reported here was to analyse the eubacterial community in the two types of bioreactor along the packed bed and during the operation time. The analysis was carried out using the 16S PCR-DGGE molecular fingerprint technique. The microbial profile analysis in the aerobic bioreactor revealed that the community was more diverse and stratified compared to those obtained in the two anoxic bioreactors, influenced by environmental factors. The main OTU involved in this process is genus Thiobacillus, although different species were detected depending on each operational condition.

Simultaneous removal of sulfur and nitrogen compounds with methane production from concentrated latex wastewater in two bioreactor zones of micro‐oxygen hybrid reactor

“…Microbial sludge samples from the SZ and AZ of the MOHR were collected to determine the microbial activities of SRB, SOB, AOB and DNB involved in biological sulfate and nitrogen compound removal processes at the end of the experiment. Na 2 SO 4 , Na 2 S 2 O 3 , NH 4 + and KNO 3 − served as substrates for analyzing SRB, SOB, AOB and DNB respectively . Furthermore, a specific methanogenic assay (SMA) was performed to analyze the activity of acetoclastic methanogens belonging to the order Methanosarcinales (MSL).…”

“…Na 2 SO 4 , Na 2 S 2 O 3 , NH 4 + and KNO 3 − served as substrates for analyzing SRB, SOB, AOB and DNB respectively. 19 Furthermore, a specific methanogenic assay (SMA) was performed to analyze the activity of acetoclastic methanogens belonging to the order Methanosarcinales (MSL). Tukey's post hoc test was applied to analyze significant differences between pairs of means at P < 0.05 using SPSS Version 18.0 (IBM, Chicago, USA).…”

“…SRB activity was higher in the SZ (0.122 g SO 4 2− -S g −1 VSS day −1 ) than in the AZ (0.105 g SO 4 2− -S g −1 VSS day −1 ). The soxB gene encoding the SoxB enzyme complex in the multi-enzyme system in sulfur oxidation 19 was used to reveal SOB abundance. The number of soxB gene copies corresponding to the SOB community was 4.60 × 10 5 and 1.62 × 10 5 copies mL −1 in the SZ and AZ respectively.…”

Application of Response Surface Methodology for H2S Removal from Biogas by a Pilot Anoxic Biotrickling Filter

“…The H 2 S concentration in the biogas stream was measured using a gas chromatograph with a thermal conductivity detector (GC-450, Bruker, Germany) and a specific gas sensor (GAsBadge®Pro, Industrial Scientific, USA) was used for H 2 S concentrations below 500 ppm V . Sulfate, nitrite and nitrate were measured by a turbidimetric method (4-500-SO 4 2− E), a colorimetric method (45000-NO 2 − B) and by an ultraviolet method (4500-NO 3 − B), respectively [17].…”

“…In biological processes the most widely used and studied microorganisms belong to the group of bacterial chemotrophic species, which use reduced sulfur compounds as an energy source and use oxygen (aerobic) or nitrate/nitrite (anoxic) as electron acceptors [3,4]. The biodesulfurization of biogas involves the use of technologies that facilitate appropriate contact between the gas and the liquid.…”