A Comparative Study of Ethylene Oxide Gas, Hydrogen Peroxide Gas Plasma, and Low-Temperature Steam Formaldehyde Sterilization

Kanemitsu, Keiji; Imasaka, Takayuki; Ishikawa, Shiho; Kunishima, Hiroyuki; Harigae, Hideo; Ueno, K.; Takemura, Hiromu; Hirayama, Yoshihiro; Kaku, Mitsuo

doi:10.1086/502572

Cited by 46 publications

(29 citation statements)

References 7 publications

(2 reference statements)

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“…For that reason, they are employed as biological indicators for monitoring the effectiveness of sterilization processes, such as vaporized hydrogen peroxide treatments (17,19), autoclaving, and UV irradiation (25,30).…”

mentioning

confidence: 99%

Applications of a Rapid Endospore Viability Assay for Monitoring UV Inactivation and Characterizing Arctic Ice Cores

Shafaat

Ponce

2006

Appl Environ Microbiol

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We have developed a rapid endospore viability assay (EVA) in which endospore germination serves as an indicator for viability and applied it to (i) monitor UV inactivation of endospores as a function of dose and (ii) determine the proportion of viable endospores in arctic ice cores (Greenland Ice Sheet Project 2 [GISP2] cores; 94 m). EVA is based on the detection of dipicolinic acid (DPA), which is released from endospores during germination. DPA concentrations were determined using the terbium ion (Tb 3؉ )-DPA luminescence assay, and germination was induced by L-alanine addition. The concentrations of germinable endospores were determined by comparison to a standard curve. Parallel EVA and phase-contrast microscopy experiments to determine the percentage of germinable spores yielded comparable results (54.3% ؎ 3.8% and 48.9% ؎ 4.5%, respectively), while only 27.8% ؎ 7.6% of spores produced CFU. EVA was applied to monitor the inactivation of spore suspensions as a function of UV dose, yielding reproducible correlations between EVA and CFU inactivation data. The 90% inactivation doses were 2,773 J/m 2 , 3,947 J/m 2 , and 1,322 J/m 2 for EVA, phase-contrast microscopy, and CFU reduction, respectively. Finally, EVA was applied to quantify germinable and total endospore concentrations in two GISP2 ice cores. The first ice core contained 295 ؎ 19 germinable spores/ml and 369 ؎ 36 total spores/ml (i.e., the percentage of germinable endospores was 79.9% ؎ 9.3%), and the second core contained 131 ؎ 4 germinable spores/ml and 162 ؎ 17 total spores/ml (i.e., the percentage of germinable endospores was 80.9% ؎ 8.8%), whereas only 2 CFU/ml were detected by culturing.

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confidence: 99%

Applications of a Rapid Endospore Viability Assay for Monitoring UV Inactivation and Characterizing Arctic Ice Cores

Shafaat

Ponce

2006

Appl Environ Microbiol

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“…Furthermore, although ETO sterilization (12/88 blend with HCFC) is considered to be the "gold standard" among low-temperature sterilization methods, it did not appear as the most effective method in our study. with complex designs (11) . These authors applied LTSF sterilization with a 37% formaline solution as a source of formaldehyde.…”

Section: Discussionmentioning

confidence: 99%

The sterilization efficacy of reprocessed single use diathermy pencils

Neto

Graziano

Padoveze

et al. 2010

Rev. Latino-Am. Enfermagem

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In Brazil, single use diathermy pencils (SUDP) are among the most common reused devices.This study assesses the sterilization efficacy of reprocessing SUDP using two cleansing methods (manual or automated), followed by one of three of the low-temperature sterilization methods: Hydrogen Peroxide Plasma (HPP), Ethylene Oxide (ETO) or Low-Temperature Steam Formaldehyde (LTSF). The sample was composed of 360 SUDP after their first use.The probability of sterilization failure was estimated considering the number of positive microbiological results obtained by cultures of the studied devices. The overall sterilization failure probability for SUDP was 0.26. The sterilization method, which presented the lowest failure probability was the LTSF (0.01), followed by ETO (0.21) and HPP (0.56). Automated cleansing obtained a better result than manual cleansing. This trial demonstrated that the probability of sterilization in reprocessed SUDP is highly dependent on both the type of cleansing and the sterilization method applied. La eficacia de la esterilización del bisturí eléctrico tipo lápiz de uso único reprocesadosEn Brasil, el bisturí eléctrico tipo lápiz de uso único (BETLUU) es uno de los artículos más comúnmente reutilizados. El presente estudio evaluó la eficacia de la esterilización de

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“…S obzirom na to da takva sterilizacija ima ograničenja te uspjeh može biti upitan kad je riječ o predmetima složene strukture (17), obavljena je kontrola sterilizacije svih 60 uzoraka. Svaki korijen bio je pohranjen u sterilnu plastičnu bočicu.…”

Section: Kontrola Sterilizacijeunclassified

“…Since plasma sterilization has limitations and the success can be questionable for items with a narrow internal diameter and complex structure (17), sterilization control was performed in all 60 samples. Each selected root was placed in a sterile plastic vial.…”

Section: Sterilization Controlmentioning

confidence: 99%

Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo System

Toljan¹,

Bago²,

Jurič

et al. 2016

Acta Stomatol Croat

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A Comparative Study of Ethylene Oxide Gas, Hydrogen Peroxide Gas Plasma, and Low-Temperature Steam Formaldehyde Sterilization

Cited by 46 publications

References 7 publications

Applications of a Rapid Endospore Viability Assay for Monitoring UV Inactivation and Characterizing Arctic Ice Cores

Applications of a Rapid Endospore Viability Assay for Monitoring UV Inactivation and Characterizing Arctic Ice Cores

The sterilization efficacy of reprocessed single use diathermy pencils

Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo System

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