A comparative study of electrical field stimulation of the guinea-pig, ferret and marmoset urinary bladder

Moss, Helen E.; Burnstock, Geoffrey

doi:10.1016/0014-2999(85)90375-9

Cited by 50 publications

(30 citation statements)

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“…The effect of desensitization to a,fi-methylene ATP was greater at stimulation frequencies < 15 Hz. This is in general agreement with previous studies of guinea-pig bladder (De Sy, 1971;Moss & Burnstock, 1985), rabbit and pig bladder (Sibley, 1984) in which atropine resistance has been observed at stimulation frequencies < 10 Hz. Creed et al (1983) have also noted that the late atropine-sensitive component of the electrical response of rabbit bladder to nerve stimulation increased in amplitude and duration with higher frequencies of stimulation.…”

Section: Discussionsupporting

confidence: 93%

“…Kasakov & Burnstock (1983) showed that a,fi-methylene ATP abolished the contractile response at stimulation frequencies > 2-4Hz. Moss & Burnstock (1985) demonstrated the effects of atropine and cx,fI-methylene ATP on the contractile responses of guinea-pig, marmoset and ferret urinary bladder: desensitization to aj-methylene ATP produced the greatest inhibition at frequencies of < 10 Hz, and with the simultaneous addition of atropine the response to field stimulation was abolished at all frequencies between 2-40 Hz. All these results, and the present data are consistent with a model in which single nerve stimuli evoke ej.ps through release of ATP, which initiate action potentials and contraction of the detrusor.…”

Section: Discussionmentioning

confidence: 98%

“…Previous studies of the mechanical responses of guinea-pig bladder muscle have suggested that co-transmission in this structure may involve acetylcholine and a non-cholinergic non-adrenergic, possibly purinergic, transmitter (Mackenzie et al, 1982;Theobald, 1982;Moss & Burnstock, 1985).…”

Section: Introductionmentioning

confidence: 96%

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Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

Brading

Mostwin

1989

British J Pharmacology

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1 The electrical and mechanical responses to transmural stimulation of intrinsic nerves have been recorded from smooth muscle strips dissected from the dome of the guinea-pig bladder, by use of intracellular microelectrodes, and conventional tension recording techniques. 2 Stimulation of intrinsic nerves evoked action potentials in all cells studied. Hyperpolarization of the cells by extracellular current injection revealed subthreshold excitatory junction potentials (ej.ps) in about a quarter of the cells studied. 3 Action potentials could still be evoked in the presence of atropine and neostigmine, but were abolished after desensitization of the cells to a,#-methylene ATP, a stable analogue of ATP.4 In the presence of neostigmine, the evoked action potential was followed by a slow depolarization of the membrane. The mechanical response increased in amplitude and duration. 5 The contractile response to transmural nerve stimulation was reduced but not abolished in the presence of either atropine or desensitizing doses of a,fi-methylene ATP. Atropine was more effective at high frequencies of stimulation () 30Hz), and a,#-methylene ATP at low frequencies ( < 15 Hz). In combination the drugs abolished the response. 6 The results suggest that the mechanical response to excitatory nerve stimulation is biphasic. The early transient reponse is elicited by ej.ps and evoked spikes, is resistant to atropine, but sensitive to desensitization of purinoceptors. The late response is mediated through muscarinic receptors, involves little membrane depolarization, and is unaffected by desensitization of purinoceptors. These responses are analogous to the responses seen in rabbit bladder, and in the sympathetically innervated rat tail artery and guinea-pig vas deferens.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 98%

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Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

Brading

Mostwin

1989

British J Pharmacology

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“…1b). Later studies have offered unequivocal support for this hypothesis (see [106]), not only in guinea-pig bladder [83,112,254,287,314,325,356,482,556,721], but also the bladders of many other species, including: mouse [4,301,682,714]; pig [253]; hamster [561]; marmoset and ferret [500]; dog [653] The prejunctional inhibition of both cholinergic and purinergic components of the nerve-mediated responses of the rat bladder by adenosine was taken as evidence in support of cotransmission ( [545]; see also [499]). Following the initial proposa1 that ATP contributed to the contractile responses of the urinary bladder to parasympathetic nerve stimulation [97], much debate followed, as indeed it did about the general concept of purinergic neurotransmission.…”

Section: Parasympathetic Cotransmissionmentioning

confidence: 99%

Purinergic signalling in the urinary tract in health and disease

Burnstock

2013

Purinergic Signalling

140

149

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Purinergic signalling is involved in a number of physiological and pathophysiological activities in the lower urinary tract. In the bladder of laboratory animals there is parasympathetic excitatory cotransmission with the purinergic and cholinergic components being approximately equal, acting via P2X1 and muscarinic receptors, respectively. Purinergic mechanosensory transduction occurs where ATP, released from urothelial cells during distension of bladder and ureter, acts on P2X3 and P2X2/3 receptors on suburothelial sensory nerves to initiate the voiding reflex, via low threshold fibres, and nociception, via high threshold fibres. In human bladder t h e p u r i n e rg i c c o m p o n e n t o f p a r a s y m p a t h e t i c cotransmission is less than 3 %, but in pathological conditions, such as interstitial cystitis, obstructed and neuropathic bladder, the purinergic component is increased to 40 %. Other pathological conditions of the bladder have been shown to involve purinoceptor-mediated activities, including multiple sclerosis, ischaemia, diabetes, cancer and bacterial infections. In the ureter, P2X7 receptors have been implicated in inflammation and fibrosis. Purinergic therapeutic strategies are being explored that hopefully will be developed and bring benefit and relief to many patients with urinary tract disorders.

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“…Excitatory purinergic responses have been reported in bladders of rabbit (Dean & Downie, 1978), guinea-pig (Burnstock et al, 1978), rat (Burnstock et al, 1972), mouse (Acevedo & Contreras, 1985), ferret and marmoset (Moss & Burnstock, 1985), and cat (Theobald, 1983). In human isolated bladder strips adenosine 5'-triphosphate (ATP) can induce concentration-dependent contractions (Husted et al, 1983;Hoyle et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

High‐ and low‐affinity binding sites for [³H]‐α,β‐methylene ATP in rat urinary bladder membranes

Burnstock

1990

British J Pharmacology

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A comparative study of electrical field stimulation of the guinea-pig, ferret and marmoset urinary bladder

Cited by 50 publications

References 15 publications

Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

Purinergic signalling in the urinary tract in health and disease

High‐ and low‐affinity binding sites for [³H]‐α,β‐methylene ATP in rat urinary bladder membranes

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A comparative study of electrical field stimulation of the guinea-pig, ferret and marmoset urinary bladder

Cited by 50 publications

References 15 publications

Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

Purinergic signalling in the urinary tract in health and disease

High‐ and low‐affinity binding sites for [3H]‐α,β‐methylene ATP in rat urinary bladder membranes

Contact Info

Product

Resources

About

High‐ and low‐affinity binding sites for [³H]‐α,β‐methylene ATP in rat urinary bladder membranes