A comparative study of commercially available, minimally invasive, sampling methods on Early Neolithic humeri analysed via palaeoproteomics

Hansen, Jakob; Dekker, Joannes; Troché, Gaudry; Fagernäs, Zandra; Olsen, Jesper V.; Saña Seguí, Maria; Welker, Frido

doi:10.1016/j.jas.2024.106002

Cited by 3 publications

(2 citation statements)

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“…More recently, proteins on skin and bones on the surfaces of cranial bone of a mummified Egyptian from the 26th dynasty (664–525 BC) have been identified using dermatology-grade skin sampling strips . Recently, Hansen et al compared different minimally invasive sampling methods and showed that bone preservation influences the proteomic result and that the bone surfaces are modified according to the specific sampling method . We describe here the low-invasive proteomics identification of Iron Age, Neolithic, and Upper Pleistocene bones from 120,000 to 150 BC based on sampling with dermatological skin tape discs.…”

Section: Introductionmentioning

confidence: 99%

“…32 Recently, Hansen et al compared different minimally invasive sampling methods and showed that bone preservation influences the proteomic result and that the bone surfaces are modified according to the specific sampling method. 33 We describe here the low-invasive proteomics identification of Iron Age, Neolithic, and Upper Pleistocene bones from 120,000 to 150 BC based on sampling with dermatological skin tape discs. First, we adapted our new sensitive digestion method based on both demineralization and digestion in 96-well plates followed by MALDI FTICR MS analysis, which has a sensitivity below the milligram of bones to dermatological skin tape-disc sampling.…”

Section: ■ Introductionmentioning

confidence: 99%

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Low-Invasive Sampling Method with Tape-Disc Sampling for the Taxonomic Identification of Archeological and Paleontological Bones by Proteomics

Fabrizi,

Flament,

Delhon

et al. 2024

J. Proteome Res.

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Collagen from paleontological bones is an important organic material for isotopic measurement, radiocarbon analysis, and paleoproteomic analysis to provide information on diet, dating, taxonomy, and phylogeny. Current paleoproteomic methods are destructive and require from a few milligrams to several tens of milligrams of bone for analysis. In many cultures, bones are raw materials for artifacts that are conserved in museums, which hampers damage to these precious objects during sampling. Here, we describe a low-invasive sampling method that identifies collagen, taxonomy, and post-translational modifications from Holocene and Upper Pleistocene bones dated to 130,000 and 150 BC using dermatological skin tape discs for sampling. The sampled bone micropowders were digested following our highly optimized enhanced filter-aided sample preparation protocol and then analyzed by MALDI FTICR MS and LC-MS/MS for identifying the genus taxa of the bones. We show that this low-invasive sampling does not deteriorate the bones and achieves results similar to those obtained by more destructive sampling. Moreover, this sampling method can be carried out at archeological sites or in museums.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Low-Invasive Sampling Method with Tape-Disc Sampling for the Taxonomic Identification of Archeological and Paleontological Bones by Proteomics

Fabrizi,

Flament,

Delhon

et al. 2024

J. Proteome Res.

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Early Holocene preservation differences between cortical and trabecular bone proteomes

Ásmundsdóttir,

Hansen,

Fagernäs

et al. 2024

Journal of Archaeological Science: Reports

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Combining traceological analysis and ZooMS on Early Neolithic bone artefacts from the cave of Coro Trasito, NE Iberian Peninsula: Cervidae used equally to Caprinae

Hansen,

Sierra,

Mata

et al. 2024

PLoS ONE

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Few studies have combined the analysis of use-wear traces, traceology, and the proteomic taxonomic identification method Zooarchaeology by Mass Spectrometry (ZooMS). Traceology provides information on the usage, in this case, of bone artefacts, while ZooMS allows for taxonomic identifications where diagnostic features are otherwise gone. The approaches therefore offer complementary information on bone artefacts, allowing for insights into species selection strategies in bone tool manufacture and their subsequent use. Here we present a case study of 20 bone artefacts, mainly bone points, from the Early Neolithic cave site of Coro Trasito located on the southern slope of the Central Pyrenees. Hitherto, studies on Early Neolithic bone artefacts from the Iberian Peninsula have suggested based on morphological assessments that Ovis aries/Capra hircus constituted the majority of the bone material selected for bone tool production. However, the taxonomic identification in this study suggests that, at this site, Cervidae was selected equally to that of O. aries/C. hircus. Furthermore, bone artefacts made from Cervidae specimens seem to be utilised in a wider range of artefact types compared to O. aries/C. hircus. Coro Trasito’s bone artefact species composition is probably site-specific to some degree, however, morphological assessments of bone artefacts might not be representative and could be biased towards certain species. Therefore, research on bone artefacts’ usage could possibly gain new insights by implementing ZooMS in combination with traceology.

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A comparative study of commercially available, minimally invasive, sampling methods on Early Neolithic humeri analysed via palaeoproteomics

Cited by 3 publications

References 44 publications

Low-Invasive Sampling Method with Tape-Disc Sampling for the Taxonomic Identification of Archeological and Paleontological Bones by Proteomics

Low-Invasive Sampling Method with Tape-Disc Sampling for the Taxonomic Identification of Archeological and Paleontological Bones by Proteomics

Early Holocene preservation differences between cortical and trabecular bone proteomes

Combining traceological analysis and ZooMS on Early Neolithic bone artefacts from the cave of Coro Trasito, NE Iberian Peninsula: Cervidae used equally to Caprinae

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