A comparative study of cellulase and hemicellulase activities of brackish water clam <i>Corbicula japonica</i> with those of other marine Veneroida bivalves

Sakamoto, Kentarou; Toyohara, Haruhiko

doi:10.1242/jeb.031567

Cited by 32 publications

(20 citation statements)

References 37 publications

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“…To understand the differences in the enzymatic digestive systems of the four bivalve species mentioned above, we evaluated the activity level of cellulase and hemicellulase prepared from the crystalline style (Sakamoto and Toyohara 2009a ties were evaluated by the plate and the reducing sugar assays. In the plate assay, substrate-containing agarose plates were used, and the activity level was determined according to the size of the unstained halo by Congo Red (Sakamoto and Toyohara 2009a).…”

Section: -5 Comparative Studies On Cellulase and Hemicellulase Actimentioning

confidence: 99%

“…In the plate assay, substrate-containing agarose plates were used, and the activity level was determined according to the size of the unstained halo by Congo Red (Sakamoto and Toyohara 2009a). In the reducing sugar assay, the degrading activities of phosphoric acid swollen cellulose (PASC), endoglucanase (CMCase, degrading activity of carboxymethyl cellulose), xylanase, b-mannanase, b-1,3-glucanase and pectinase were measured.…”

Section: -5 Comparative Studies On Cellulase and Hemicellulase Actimentioning

confidence: 99%

“…In the reducing sugar assay, the degrading activities of phosphoric acid swollen cellulose (PASC), endoglucanase (CMCase, degrading activity of carboxymethyl cellulose), xylanase, b-mannanase, b-1,3-glucanase and pectinase were measured. Further details of the assay methods were described by Sakamoto and Toyohara (2009a).…”

Section: -5 Comparative Studies On Cellulase and Hemicellulase Actimentioning

confidence: 99%

“…Amounts of reducing sugar produced from 1% substrate solution (carboxymethylcellulose, locust bean gum, xylan, glyloid 3S, or lichenin, respectively) were measured by reducing sugar assay. From Sakamoto and Toyohara (2009a). and Caridina multidentata (Fig.…”

Section: -1 Cellulase and Hemicellulase Distribution In Wetlandsmentioning

confidence: 99%

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Degradation of Plant-derived Carbohydrates in Wetlands

Ogino¹,

Liu²,

Toyohara³

2018

Aqua-BioSci. Monogr. (ABSM)

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Corbicula japonica is one of the most important bivalves in inland fishery resources. Stable isotopic studies have shown that it can assimilate plant-derived hard-degradable carbohydrates. Further studies revealed that this species has endogenous enzymes to digest these carbohydrates.We investigated the ability of various organisms to degrade hard-degradable carbohydrates in wetlands from subarctic to subtropical zones and found that they could contribute to their degradation.We found that the enzymes secreted from organisms would bind to the sediment and contribute to the degradation of cellulose. We defined these enzymes as "the environmental enzymes". Comparison of various sediments revealed that the binding abilities of the sediment for the environmental enzyme were ascribable to oxidized metal, organic matters and the ratio of sand, silt and clay.

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Section: -5 Comparative Studies On Cellulase and Hemicellulase Actimentioning

confidence: 99%

Section: -5 Comparative Studies On Cellulase and Hemicellulase Actimentioning

confidence: 99%

Section: -5 Comparative Studies On Cellulase and Hemicellulase Actimentioning

confidence: 99%

Section: -1 Cellulase and Hemicellulase Distribution In Wetlandsmentioning

confidence: 99%

See 2 more Smart Citations

Degradation of Plant-derived Carbohydrates in Wetlands

Ogino¹,

Liu²,

Toyohara³

2018

Aqua-BioSci. Monogr. (ABSM)

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“…This abundant by-product of palm oil industry is used as animal feed. Due to its high content of hemicellulose (mannan and galactomanan) and low essential amino acids contents, the use of palm kernel cake in animal feed is limited to ruminant animals (Yopi et al, 2006, Sakamoto & Toyohara, 2009.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Bacterial Mannanase for Hydrolyzing Palm Kernel Cake to Produce Manno-oligosaccharides Prebiotics

Utami¹,

Meryandini²,

Wiryawan³

2013

Med Pet

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Palm kernel cake (PKC) is a promising source of prebiotics, since it contains high amount of β-mannan which can be further hydrolyzed to manno-oligasaccharides (MOS), a prebiotic. Therefore, this research was carried out to analyze the capability of a bacterial isolate (A2 isolates) previously isolated from soils sample from around IPB campus to hydrolyze PKC. Based on 16S-DNA analysis, isolate A2 was identified as Brevibacillus borstelensis. Mannanase of A2 isolate had an optimum condition at 90 o C and pH 7. Mannanase activity of crude extracts using Locust Bean Gum (LBG) and PKC as substrates were 0.37U/mL and 0.032U/mL, respectively. However, the most favorable production of oligosaccharides based on the degree of polymerization was obtained after 72-h of incubation with the ratio of substrate:enzyme, 1.2:1, on 1.5% PKC as substrate. The manno-oligosaccharides prebiotic obtained was found to interfere the growth of both lactic acid bacteria (Lactobacillus casei) and pathogenic microflora (Escherichia coli). E. coli apparently could not use this prebiotic as the carbon sources, in contrast to L. casei. Substitution of carbon source in medium with prebiotics reduced the capability of L. casei to produce organic acids. It is concluded that local A2 isolate (B. borstelensis) produces mannanase which can be used to produce prebiotics from PKC. Key words: Brevibacillus borstelensis, mannanase, Lactobacillus casei, PKC ABSTRAKKomponen utama dari bungkil inti sawit (BIS) adalah β-manan yang dapat digunakan dalam produksi prebiotik Manno-oligosakarida (MOS). Penelitian ini bertujuan mengkarakterisasi kemampuan isolat bakteri A2 yang diisolasi dari sampel tanah di sekitar kampus IPB dalam mendegradasi BIS. Berdasarkan analisis 16S-DNA isolat A2 teridentifikasi sebagai Brevibacillus borstelensis. Mananase isolat A2 memiliki kondisi optimum pada suhu 90 o C dan pH 7. Aktivitas mananase isolat A2 pada media dengan Locust Bean Gum (LBG) dan BIS berturut turut adalah 0.37U/ml dan 0.032U/ml. Perbandingan substrat:enzyme: 1.2:1, pada substrat BIS 1.5% memberikan derajat polimerisasi terbaik bagi produksi oligosakarida. Prebiotik MOS ini dapat mempengaruhi pertumbuhan Escherichia coli patogen dan Lactobacillus casei. E. coli tidak dapat menggunakan prebiotik ini sebagai sumber karbon dibandingkan L. casei. Substitusi sumber karbon dengan prebiotik menurunkan kemampuan L. casei dalam memproduksi asam organik. Disimpulkan bahwa isolat lokal A2 (B. borstelensis) menghasilkan mananase yang dapat digunakan untuk menghasilkan prebiotik dari BIS. Kata kunci: Brevibacillus borstelensis, mananase, Lactobacillus casei, PKC

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Rice straw management: the big waste

Domínguez-Escribá

Porcar

2009

Biofuels Bioprod Bioref

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Rice is one of the major foods, with consumption per capita of 65 kg per year, accounting for 20% of global ingested calories. Rice production is expected to increase signifi cantly in the near future in order to feed the rising human population. Today, paddy rice culture produces 660 million tons of rice, along with 800 million dry tons of agricultural residues, mainly straw. This biomass is managed predominantly through rice straw burning (RSB) and soil incorporation strategies. RSB leads to signifi cant air pollution and has been banned in some regions, whereas stubble and straw incorporation into wet soil during land preparation is associated with enhanced methane emissions. Therefore, both strategies have important deleterious environmental effects and fail to take advantage of the huge energy potential of rice straw. Using rice straw as lignocellulosic biomass to produce bioethanol would appear to be a promising and ambitious goal to both manage this agricultural waste and to produce environmentally friendly biofuel. Technical diffi culties, however, associated with the conversion of lignocellulose into simple, fermentable sugars, have hampered the massive development of rice-straw-derived bioethanol. Recent technical advances in straw pre-treatment, hydrolysis and fermentation may, however, overcome these limitations and facilitate a dramatic turnover in biofuels production in the near future. R ice is a key crop for human consumption. In 2008, 155 million hectares worldwide were devoted to rice culture. Th is area has only slightly increased in the last 30 years, but paddy rice production almost doubled in the same period, mainly due to large-scale adoption of improved varieties. Th ere are more than 100 000 cultivated varieties of rice belonging to only two species: Oryza sativa, the Asian cultivated rice grown all over the world; and O. glaberrima, an African cultivated rice limited to West Africa. Varieties of O. sativa are classifi ed into two major groups: (i) indica, which includes most of the varieties of the tropical ecotype; and (ii) japonica, representing the temperate ecotype. Today, more than 660 million tons of rice are produced every year and rice accounts for 20% of total ingested calories (30% in Asia). In certain developing countries, such as Bangladesh or Cambodia, consumption can reach more than 70%. Rice consumption per capita was 50 kg in 1960; 57 kg in 1978; and about 65 kg in 2008. Th is increasing rate of Dr Laura Domínguez-Escribà Laura Domínguez-Escribà is a researcher in the

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A comparative study of cellulase and hemicellulase activities of brackish water clam Corbicula japonica with those of other marine Veneroida bivalves

Cited by 32 publications

References 37 publications

Degradation of Plant-derived Carbohydrates in Wetlands

Degradation of Plant-derived Carbohydrates in Wetlands

Characterization of Bacterial Mannanase for Hydrolyzing Palm Kernel Cake to Produce Manno-oligosaccharides Prebiotics

Rice straw management: the big waste

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