A Comparative Study of Artificial Ceroid/Lipofuscin from Different Tissue Materials of Rats

Abstract

The artificial ceroid/lipofuscin pigments originated from different organ tissues, including liver, brain, heart, and kidney of rats, and biomaterials were studied with improved fluorometric techniques. With all tissue materials exposed under ultraviolet (UV) light, a series of similar fluorescent colors were observed under microfluorometer. Analogous fluorescence spectra were also demonstrated with a three-dimensional (3-D) front-surface fluorometric technique despite of the tissue differences. Measured with 3-D fluorometry, relatively simple lipofuscin-like fluorophores were observed from the reactions of malondialdehyde (MDA) with critical biological macromolecules, such as bovine serum albumin (BSA) and DNA. Our results demonstrated that the biomaterials from different tissues have a similar fate under accelerated oxidative/carbonyl stresses but may be differentiated by a fluorescence intensity ratio.

Oxidative damage in multiple sclerosis lesions

“…In the current study, immunocytochemistry for oxidized lipids revealed their presence within macrophages, located in structures that morphologically resemble lipofuscin. This is not surprising, since lipofuscin appears to contain non-digestible remnants of oxidized cellular components, accumulating in macrophages or resident cells of the CNS, for instance neurons (Keller et al , 2004; Wang et al , 2008). Similarly, nitrosylated epitopes, recognized by antibodies against nitrotyrosine, were mainly seen in macrophages (Cross et al , 1998; Liu et al , 2001).…”

Estradiol Modulates Membrane-Linked ATPases, Antioxidant Enzymes, Membrane Fluidity, Lipid Peroxidation, and Lipofuscin in Aged Rat Liver

“…Ample evidence showed that peroxidative damage to lipid and protein occurs with the aging process and that the products of these reactions accumulate with age [2, 27, 28, 50, 51]. Aging rats treated with E2 reversed the MDA levels to almost normal levels in the liver, suggesting its anti-lipidperoxidative abilities [8, 49].…”

Autofluorescence spectroscopy and imaging: a tool for biomedical research and diagnosis

“…The nature of the lipofuscin-like lipopigments as peroxidized products of lipids and other intracellular macromolecules was supported by later investigations, even leading to their proposal as AF biomarkers of oxidative damages induced in livers with lipid accumulation and oxidative stress. 87 , 127 , 173 , 174 Actually, an increasing number of endogenous fluorophores has been considered for comprehensive AF based diagnostic applications in hepatology, depending on the complex biochemical composition characterizing the liver tissue due to its engagement in several metabolic and detoxificating functions. 84-87 , 175 In addition to NAD(P)H, flavins and lipofuscins, the overall liver tissue AF emission can originate from proteins, vitamin A and fatty acids likely playing a role as interconnected autofluorescent biomarkers of liver disorders.…”