A Comparative Study of Approaches to Improve the Sensitivity of Lateral Flow Immunoassay of the Antibiotic Lincomycin

Abstract

This study provides a comparative assessment of the various nanodispersed markers and related detection techniques used in the immunochromatographic detection of an antibiotic lincomycin (LIN). Improving the sensitivity of the competitive lateral flow immunoassay is important, given the increasing demands for the monitoring of chemical contaminants in food. Gold nanoparticles (AuNPs) and CdSe/ZnS quantum dots (QDs) were used for the development and comparison of three approaches for the lateral flow immunoassay (LFIA) of LIN, namely, colorimetric, fluorescence, and surface-enhanced Raman spectroscopy (SERS)-based LFIAs. It was demonstrated that, for colorimetric and fluorescence analysis, the detection limits were comparable at 0.4 and 0.2 ng/mL, respectively. A SERS-based method allowed achieving the gain of five orders of magnitude in the assay sensitivity (1.4 fg/mL) compared to conventional LFIAs. Therefore, an integration of a SERS reporter into the LFIA is a promising tool for extremely sensitive quantitative detection of target analytes. However, implementation of this time-consuming technique requires expensive equipment and skilled personnel. In contrast, conventional AuNP- and QD-based LFIAs can provide simple, rapid, and inexpensive point-of-care testing for practical use.

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“…In addition, the low versatility of the latex nanoparticles inhibits the development of further strategies to combine the strip with transducers. To enhance the sensitivity, the traditional AuNPs label has been replaced by an AuNP nanocomposite and novel nanoparticle, including AuNP-decorated silica nanorods, quantum dots, fluorescence-quenching, and particles used in other emerging detection principles such as magnetic systems [67]. For example, Lu et al reported two chromatographic immunoassays to detect Stx, using the signal la-bels colloidal AuNPs and CdTe QDs, respectively, to reduce the detection limit [68].…”

Terbium-based dual-ligand metal organic framework by diffusion method for selective and sensitive detection of danofloxacin in aqueous medium

“…Despite the signi cant advantages of sensitivity and selectivity, these techniques are time-consuming, labour-intensive, and expensive to operate due to the need for specialized equipment, extensive pre-treatment, and high operating costs. As a result, these methods are di cult to devise for on-site monitoring of FQs (Serebrennikova et al 2020). Fluorescence detection thus offers a substitute for high selectivity, sensitivity, and accuracy, as well as low cost, quick and easy operation for detection of antibiotics.…”

A New Covalent Organic Framework of Dicyandiamide-Benzaldehyde Nanocatalytic Amplification SERS/RRS Aptamer Assay for Ultratrace Oxytetracycline with the Nanogold Indicator Reaction of Polyethylene Glycol 600

“…Surface-enhanced Raman scattering (SERS) is a sensitive molecular spectral technique. Generally, the nanostructured substrates functionalize with Raman reporter molecules, and strong characteristic peaks are generated in the SERS spectrum, thus achieving quantitative detection of the analyte [10]. SERS substrates usually include gold, silver, and other noble metal materials, and the signal intensity of SERS can be effectively enhanced by improving the substrate [11].…”