A comparative study of anti-Trypanosoma cruzi serum obtained in acute and chronic phase of infection in mice

Takehara, H.A.; Silva, Ana M.M. da; Brodskyn, Cláudia; Mota, I.

doi:10.1016/0165-2478(89)90117-x

Cited by 8 publications

(2 citation statements)

References 8 publications

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“…K1 (an amino-terminal peptide from this protein) also induced significant reactivity in both groups of Colombian chronic chagasic patients [29]. The data observed here are consistent with that previously described in humans and animal models where high levels of total IgG specific response has been a hallmark of the sickness' chronic stages [30,31]. …”

Section: Discussionsupporting

confidence: 89%

Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients

et al. 2009

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BackgroundAntigen specificity and IgG subclass could be significant in the natural history of Chagas' disease. The relationship between the different stages of human Chagas' disease and the profiles of total IgG and its subclasses were thus analysed here; they were directed against a crude T. cruzi extract and three recombinant antigens: the T. cruzi kinetoplastid membrane protein-11 (rKMP-11), an internal fragment of the T. cruzi HSP-70 protein192-433, and the entire Trypanosoma rangeli HSP-70 protein.MethodsSeventeen Brazilian acute chagasic patients, 50 Colombian chronic chagasic patients (21 indeterminate and 29 cardiopathic patients) and 30 healthy individuals were included. Total IgG and its subtypes directed against the above-mentioned recombinant antigens were determined by ELISA tests.ResultsThe T. cruzi KMP-11 and T. rangeli HSP-70 recombinant proteins were able to distinguish both acute from chronic chagasic patients and infected people from healthy individuals. Specific antibodies to T. cruzi crude antigen in acute patients came from IgG3 and IgG4 subclasses whereas IgG1 and IgG3 were the prevalent isotypes in indeterminate and chronic chagasic patients. By contrast, the specific prominent antibodies in all disease stages against T. cruzi KMP-11 and T. rangeli HSP-70 recombinant antigens were the IgG1 subclass.ConclusionT. cruzi KMP-11 and the T. rangeli HSP-70 recombinant proteins may be explored together in the immunodiagnosis of Chagas' disease.Polarising the IgG1 subclass of the IgG response to T. cruzi KMP-11 and T. rangeli HSP-70 recombinant proteins could have important biological effects, taking into account that this is a complement fixing antibody.

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Section: Discussionsupporting

confidence: 89%

Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients

et al. 2009

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“…The mechanism(s) by which host genes influence resistance to T. cruzi infection are not understood but there is substantial evidence that protective immune responses are genetically regulated. Several studies, employing a variety of experimental approaches, have indicated that antibody is important in resistance (Culbertson & Kolodny 1938, Kierszenbaum & Howard 1976, Rodriguez, et al 1981, Brener 1986), however, the isotype(s) and specificity(ies) of the relevant anti-T. cruzi antibodies remain unresolved (Takehara et al 1981, Romeiro, Takehara & Mota 1984, Brener 1986, Takehara et al 1989, Juri et al 1990. We found that different strains of mice produce different levels, specificities, and isotypes of parasite reactive antibodies during acute infection and that these differences correlate with the expression of MHC and/or background genes.…”

Section: Introductionmentioning

confidence: 99%

Host genetics and resistance to acute Trypanosoma cruzi infection in mice. I. Antibody isotype profiles

Powell

Wassom

1993

Parasite Immunology

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Some strains of inbred mice survive acute infection with Trypanosoma cruzi while others die within a few weeks after infection. Mice which express B10 background genes and either the H-2q or H-2d haplotypes are resistant and survive. However, mice which share the B10 genetic background but express H-2k alleles die, usually within 4 weeks following infection. These data confirm that at least 1 gene in the major histocompatibility complex can determine whether an animal lives or dies during the acute phase. Expression of the H-2q haplotype on the B10 genetic background or in DBA/1 mice is associated with resistance, but H-2q mice expressing the C3H background are susceptible. Therefore, at least 1 gene in the genetic background also influences resistance. Our data suggest that genes associated with resistance must be present in both the MHC and the genetic background or the animal will die. The isotypes and specificities of parasite reactive antibodies found in the serum of different inbred mouse strains were assessed during acute infection. Levels of IgM were higher in sera from mice which express the resistant B10 background than in sera from mice expressing the susceptible C3H background. Conversely, mice which share the C3H background genes produced high levels of anti-parasite IgG2a when compared to B10 congenic strains. Antigen specificity, however, may be influenced by both background and MHC genes, as congenic strains expressing different MHC haplotypes recognized different constellations of T. cruzi antigens.(ABSTRACT TRUNCATED AT 250 WORDS)

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Changes in isotype composition and antigen recognition of anti-Trypanosoma cruzi antibodies from acute to chronic Chagas disease

Umezawa

Shikanai‐Yasuda

Stolf³

1996

J. Clin. Lab. Anal.

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A comparative study of anti-Trypanosoma cruzi serum obtained in acute and chronic phase of infection in mice

Cited by 8 publications

References 8 publications

Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients

Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients

Host genetics and resistance to acute Trypanosoma cruzi infection in mice. I. Antibody isotype profiles

Changes in isotype composition and antigen recognition of anti-Trypanosoma cruzi antibodies from acute to chronic Chagas disease

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