A COMPARATIVE STUDY OF ALDOSTERONE AND OTHER ADRENAL STEROIDS IN ADRENALECTOMIZED DOGS¹

Increase of Plasma 17-Hydroxycorticosteroids by Cerebral Cortical and Amygdaloid Stimulation in the Cat

“…In these investigations the adrenocortical function was measured by indirect methods such as eosinophil and lymphocyte counts. However, the reliability of such indirect indices has been seriously questioned (Soylemezoglu & Wells, 1951;Bibile, 1953;Jenkins, Laidlow, Reddy, Mills & Thorn, 1953;Swingle, Eisler, Ben, Maxwell, Baker & Lebrie, 1954). Also, in the cat, the experimental animal used in the present study, no constant relationship was found between the eosinophil count and the plasma level of 17-hydroxycorticosteroids (17-OHCS) which is considered a specific index of adreno¬ cortical activity (Kaada, Setekleiv & Skaug, 1959).…”

Aldosterone and Renal Potassium Excretion

“…An examination of the published reports on which these early statements were based shows: (1) only small quantities of aldosterone were available for experimental use up to 1956 and the number of experi ments performed and subjects treated with aldosterone were relatively few; (2) aldoste rone almost uniformly had a sodium-retain ing effect when administered, and (3) the effects of aldosterone administration on po tassium metabolism were at times reported only in terms of changes in plasma potas sium concentration [83,84], In those few reports made prior to 1956 in which aldosterone was administered and in which renal potassium excretion was measured or was calculatable from sodium excretion and urinary Na/K ratios, the re sults do not appear to support a conclusion that aldosterone acted to increase potassium excretion. We summarize these reports in the following paragraphs.…”

The Physiological Basis for a Method of Assaying Aldosterone in Extracts of Human Urine

“…In the dog it is also easy 'to administer test-substances intravenously, thereby avoiding confusion arising from the fact that different steroids have different rates of absorption from extravascular tissues and that this might affect their apparent relative potencies under the conditions of a given assay, a possibility recently re-emphasized by Swingle and his co-workers (13 Characteristics of the dose-response In response to each of the three steroid preparations, there occurred both a decrease in excretion of sodium and an increase in excretion of potassium ( Figure 1). Consistent changes were not noted during the first hour following treatment.…”