A comparative study of 3 alternative avian toxicity testing methods: Effects on hepatic gene expression in the chicken embryo

Mittal, Krittika; Crump, Doug; Basu, Niladri

doi:10.1002/etc.4555

Cited by 7 publications

(9 citation statements)

References 58 publications

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“…Therefore, the chicken embryo serves as a promising in vivo model for the evaluation of nanotoxicity, considering the ethical, practical, and technical limits posed on conventional rodent and large animal experiments. 17 …”

Section: Introductionmentioning

confidence: 99%

Effects of Particle Size and Surface Charge on Mutagenicity and Chicken Embryonic Toxicity of New Silver Nanoclusters

Zhang

Wang

et al. 2022

ACS Omega

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Though there are many toxicological studies on metal nanoparticles (NPs), it remains difficult to explain discrepancies observed between studies, largely due to the lack of positive controls and disconnection between physicochemical properties of nanomaterials with their toxicities at feasible exposures in a specified test system. In this study, we investigated effects of particle size and surface charge on in vitro mutagenic response and in vivo embryonic toxicity for newly synthesized silver nanoclusters (AgNCs) at human or environmental relevant exposure and compared the new findings with one of the most common nanoscale particles, titanium dioxide NPs (TiO 2 NPs as a positive control). We hypothesized that the interaction of the test system and physicochemical properties of nanomaterials are critical in determining their toxicities at concentrations relevant with human or environmental exposures. We assessed the mutagenicity of the AgNCs (around 2 nm) and two sizes of TiO 2 NPs (i.e., small: 5–15 nm, big: 30–50 nm) using a Salmonella reverse mutation assay (Ames test). The smallest size of AgNCs showed the highest mutagenic activity with the Salmonella strain TA100 in the absence and presence of the S9 mixture, because the AgNCs maintained the nano-size scale in the Ames test, compared with two other NPs. For TiO 2 NPs, the size effect was interfered by the agglomeration of TiO 2 NPs in media and the generation of oxidative stress from the NPs. The embryonic toxicity and the liver oxidative stress were evaluated using a chicken embryo model at three doses (0.03, 0.33, and 3.3 μg/g egg), with adverse effects on chicken embryonic development in both sizes of TiO 2 NPs. The non-monotonic response was determined for developmental toxicity for the tested NPs. Our data on AgNCs was different from previous findings on AgNPs. The chicken embryo results showed some size dependency of nanomaterials, but they were more well correlated with lipid peroxidation (malondialdehyde) in chicken fetal livers. A different level of agglomeration of TiO 2 NPs and AgNCs was observed in the assay media of Ames and chicken embryo tests. These results suggest that the test nanotoxicities are greatly impacted by the experimental conditions and the nanoparticle’s size and surface charge.

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Section: Introductionmentioning

confidence: 99%

Effects of Particle Size and Surface Charge on Mutagenicity and Chicken Embryonic Toxicity of New Silver Nanoclusters

Zhang

Wang

et al. 2022

ACS Omega

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“…Genes associated with lipid/cholesterol/bile homeostasis showed distinct dysregulation between sites with high (HAM, MSI, and MAN) and low PBDE burdens (BUF, MHK, and MUD). For example, liver basic fatty acid binding protein ( Lbfabp ), which enhances fatty acid metabolism, was downregulated at HAM, MSI, and MAN, whereas its expression was upregulated at sites with lower PBDE burdens (BUF, MHK, and MUD). The downregulation of Lbfabp was also observed after exposure of chicken embryonic hepatocytes to DCCO egg extracts from HAM .…”

Section: Resultsmentioning

confidence: 99%

Toxicogenomic Assessment of Complex Chemical Signatures in Double-Crested Cormorant Embryos from Variably Contaminated Great Lakes Sites

Xia

Crump

Chiu

et al. 2020

Environ. Sci. Technol.

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Using omics approaches to monitor complex environmental mixtures is challenging. Previously, we evaluated in vitro transcriptomic effects of complex organic extracts derived from avian eggs. However, there is a lack of studies using wild species that are naturally exposed to contaminant mixtures. Here, we examined polychlorinated biphenyl (PCB) and polybrominated diphenyl ether (PBDE) residues and gene expression in embryonic liver tissue of double-crested cormorants (Phalacrocorax auritus) collected from six variably contaminated colonies. Colonies near industrialized areas were distinguished from less contaminated sites based on their PCB and PBDE concentrations. The most variably expressed genes between sites were involved in pathways including, xenobiotic metabolism (e.g., Cyp1a4), lipid/bile acid homeostasis (e.g., Lbfabp), and oxidative stress (e.g., Mt4). Hierarchical clustering, based on relative gene expression, revealed a grouping pattern similar to chemical residue concentrations. Further, partial least squares regression analysis was used to estimate chemical concentrations from transcriptomics data. PCB 155 and BDE 47 showed the highest slopes (0.77 and 0.69, respectively) fitted by linear regression of measured and estimated chemical concentrations. The application of transcriptomics to a wild avian species, naturally exposed to complex chemical mixtures and other stressors, represents a promising means to distinguish and prioritize variably contaminated sites.

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“…Chicken eggs have been well established as a model for drug screening and toxicity testing [53][54][55][56][57] . In this model, the treatment could be performed readily with the administration of reagents to the chorioallantoic membrane, and the opening of the eggshell can be easily sealed afterward, allowing for repeated drug applications.…”

Section: Discussionmentioning

confidence: 99%

Development of a potent embryonic chick lens model for studying congenital cataracts in vivo

Quan

et al. 2021

Commun Biol

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Congenital cataracts are associated with gene mutations, yet the underlying mechanism remains largely unknown. Here we reported an embryonic chick lens model that closely recapitulates the process of cataract formation. We adopted dominant-negative site mutations that cause congenital cataracts, connexin, Cx50E48K, aquaporin 0, AQP0R33C, αA-crystallin, CRYAA R12C and R54C. The recombinant retroviruses containing these mutants were microinjected into the occlusive lumen of chick lenses at early embryonic development. Cx50E48K expression developed cataracts associated with disorganized nuclei and enlarged extracellular spaces. Expression of AQP0R33C resulted in cortical cataracts, enlarged extracellular spaces and distorted fiber cell organization. αA crystallin mutations distorted lens light transmission and increased crystalline protein aggregation. Together, retroviral expression of congenital mutant genes in embryonic chick lenses closely mimics characteristics of human congenital cataracts. This model will provide an effective, reliable in vivo system to investigate the development and underlying mechanism of cataracts and other genetic diseases.

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A comparative study of 3 alternative avian toxicity testing methods: Effects on hepatic gene expression in the chicken embryo

Cited by 7 publications

References 58 publications

Effects of Particle Size and Surface Charge on Mutagenicity and Chicken Embryonic Toxicity of New Silver Nanoclusters

Effects of Particle Size and Surface Charge on Mutagenicity and Chicken Embryonic Toxicity of New Silver Nanoclusters

Toxicogenomic Assessment of Complex Chemical Signatures in Double-Crested Cormorant Embryos from Variably Contaminated Great Lakes Sites

Development of a potent embryonic chick lens model for studying congenital cataracts in vivo

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