A Comparative study for striatal-direct and -indirect pathway neurons to DA depletion-induced lesion in a PD rat model

Zheng, Xuefeng; Wu, Jiajia; Zhu, Yanhua; Chen, Si; Chen, Zhi; Chen, Tao; Huang, Ziyun; Wei, Jia-You; Li, Yanmei; Lei, Wanlong

doi:10.1016/j.neuint.2018.04.005

Cited by 6 publications

(4 citation statements)

References 52 publications

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“…H116; Sigma-Aldrich; Merck KGaA) in the right MFB [anterior-posterior (AP), -3.6; medial-lateral (ML), −0.19; and dorsal ventral (DV), -8.2] using a 10 µ l syringe (Hamilton Company). The PD rat models used in the present study were described in a previous report (22). The rats in the IA group were injected with 1 µ l of 45 nM IA (CAS no.…”

Section: Methodsmentioning

confidence: 99%

“…The primers were as follows: Caspase-3 forward, 5′-GGA CCT GTG GACCTG AAA AA-3′; Caspase-3 reverse, 5′-GCA TGC CAT ATC ATC GTC AG-3′; β-actin forward, 5′-GAA CCC TAA GGC C AA C-3′; and β-actin reverse, 5′-TGT CAC GCA CGA TT T CC-3′. The cycling conditions were previously described (22). The melting curves were analyzed using the 7500 system SDS software v.2.0.6 (Applied Biosystems™; Thermo Fisher Scientific, Inc.; cat.…”

Section: Methodsmentioning

confidence: 99%

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Partial�decortication ameliorates dopamine depletion‑induced striatal neuron lesions in rats

et al. 2019

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The balance between glutamate (cortex and thalamus) and dopamine (substantia nigra) inputs on striatal neurons is of vital importance. Dopamine deficiency, which breaks this balance and leads to the domination of cortical glutamatergic inputs, plays an important role in Parkinson's disease (PD). However, the exact impact on striatal neurons has not been fully clarified. Thus, the present study aimed to characterize the influence of corticostriatal glutamatergic inputs on striatal neurons after decortication due to dopamine depletion in rats. 6-Hydroxydopamine was injected into the right medial forebrain bundle to induce dopamine depletion, and/or ibotenic acid into the primary motor cortex to induce decortication. Subsequently, the grip strength test and Morris water maze task indicated that decortication significantly shortened the hang time and the latency that had been increased in the rats subjected to dopamine depletion. Golgi staining and electron microscopy analysis showed that the total dendritic length and dendritic spine density of the striatal neurons were decreased in the dopamine-depleted rats, whereas decortication alleviated this damage. Immunohistochemistry analysis demonstrated that decortication decreased the number of caspase-3-positive neurons in the dopamine-depleted rats. Moreover, reverse transcription-quantitative PCR and western blot analyses showed that decortication offset the upregulation of caspase-3 at both the protein and mRNA levels in the dopamine-depleted rats. In conclusion, the present study demonstrated that a relative excess of cortical glutamate inputs had a substantial impact on the pathological processes of striatal neuron lesions in PD.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Partial�decortication ameliorates dopamine depletion‑induced striatal neuron lesions in rats

et al. 2019

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“…The thicknesses of the pre-synaptic and postsynaptic membrane of symmetrical synapses were essentially equal, but the post-synaptic membranes of asymmetric synaptic contacts was significantly thicker than the pre-synaptic membrane, as shown by the thick post-synaptic density (PSD). For detailed count methods please refer to our previous paper (Lei et al, 2013;Ma et al, 2014a,b;Zheng et al, 2018Zheng et al, , 2021. The investigators were blinded for the morphological studies.…”

Section: Data Collection and Statistical Analysismentioning

confidence: 99%

A neural tract tracing study on synaptic connections for cortical glutamatergic terminals and cervical spinal calretinin neurons in rats

Huang

Sun

Zheng

et al. 2023

Front. Neural Circuits

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The cerebral cortex innervates motor neurons in the anterior horn of the spinal cord by regulating of interneurons. At present, nerve tracing, immunohistochemistry, and immunoelectron microscopy are used to explore and confirm the characteristics of synaptic connections between the corticospinal tract (CST) and cervical spinal calretinin (Cr) interneurons. Our morphological results revealed that (1) biotinylated dextran amine labeled (BDA+) fibers from the cerebral cortex primarily presented a contralateral spinal distribution, with a denser distribution in the ventral horn (VH) than in the dorsal horn (DH). An electron microscope (EM) showed that BDA+ terminals formed asymmetric synapses with spinal neurons, and their mean labeling rate was not different between the DH and VH. (2) Cr-immunoreactive (Cr+) neurons were unevenly distributed throughout the spinal gray matter, and were denser and larger in the VH than in the DH. At the single labeling electron microscope (EM) level, the labeling rate of Cr+ dendrites was higher in the VH than in the DH, in which Cr+ dendrites mainly received asymmetric synaptic inputs, and between the VH and DH. (3) Immunofluorescence triple labeling showed obvious apposition points among BDA+ terminals, synaptophysin and Cr+ dendrites, with a higher density in the VH than in the DH. (4) Double labeling in EM, BDA+ terminals and Cr+ dendrites presented the same pattern, BDA+ terminals formed asymmetric synapses either with Cr+ dendrites or Cr negative (Cr-) dendrites, and Cr+ dendrites received either BDA+ terminals or BDA- synaptic inputs. The average percentage of BDA+ terminals targeting Cr+ dendrites was higher in the VH than in the DH, but the percentage of BDA+ terminals targeting Cr- dendrites was prominently higher than that targeting Cr+ dendrites. There was no difference in BDA+ terminal size. The percentage rate for Cr+ dendrites receiving BDA+ terminal inputs was lower than that receiving BDA- terminal inputs, and the BDA+ terminal size was larger than the BDA- terminal size received by Cr+ dendrites. The present morphological results suggested that spinal Cr+ interneurons are involved in the regulatory process of the cortico-spinal pathway.

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“…VGlut1 paints the portrait of cortical connections, its expression a badge of honor for neurons weaving their intricate patterns within the striatum. Meanwhile, the thalamic afferents, started from the parafascicular nucleus (PFn) and journeying through enigmatic pathways, is narrated by the resonating presence of VGlut2 [ 4 ]. In the wake of striatal DA neuronal degeneration, a delicate equilibrium falters, and the once harmonious tango between dopaminergic and glutamatergic afferents transforms into a discordant cadence.…”

Section: Introductionmentioning

confidence: 99%

Morphological changes in perisynaptic astrocytes induced by dopamine neuronal degeneration in the striatum of rats

Sun,

Zheng,

Che

et al. 2024

Heliyon

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A Comparative study for striatal-direct and -indirect pathway neurons to DA depletion-induced lesion in a PD rat model

Cited by 6 publications

References 52 publications

Partial�decortication ameliorates dopamine depletion‑induced striatal neuron lesions in rats

Partial�decortication ameliorates dopamine depletion‑induced striatal neuron lesions in rats

A neural tract tracing study on synaptic connections for cortical glutamatergic terminals and cervical spinal calretinin neurons in rats

Morphological changes in perisynaptic astrocytes induced by dopamine neuronal degeneration in the striatum of rats

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