A comparative study for lipase immobilization onto alginate based composite electrospun nanofibers with effective and enhanced stability

Doğaç, Yasemin İspirli; Deveci, İlyas; Mercimek, Bedrettin; Teke, Mustafa

doi:10.1016/j.ijbiomac.2016.11.120

Cited by 66 publications

(15 citation statements)

References 30 publications

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“…The vials were immersed in liquid nitrogen for 5 min and quickly warmed at 37°C for 5 min three times. 17 The vials were then centrifuged at 1000× g for 20 min at 4°C (Centrifuge 5424R, Eppendorf, Germany). The supernatant was aspirated and stored at −80°C for the analysis of growth factors.…”

Section: Methodsmentioning

confidence: 99%

Promotion of skin fibroblasts collagen synthesis by polydioxanone mats combined with concentrated growth factor extracts

et al. 2019

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The purpose of this study was to combine polydioxanone and concentrated growth factor extracts to enhance the stimulation of fibroblast collagen synthesis by polydioxanone in facial plastic. In this experiment, polydioxanone was prepared as a nanoscale bioresorbable scaffold by electrospinning. The PDO-CGF mat was prepared by loading the concentrated growth factor extract onto the polydioxanone mat by lyophilization after soaking. The diameter of the mat fibers was observed by scanning electron microscopy, and the mechanical properties, biocompatibility and cytotoxicity of the material were analyzed. The release rate of transforming growth factor-beat1 (TGF-β1) in the PDO-CGF mat was evaluated by enzyme-linked immunosorbent assay (ELISA), and the amount of collagen synthesis was evaluated by real-time polymerase chain reaction (RT-PCR) and immunofluorescence. The results showed that the PDO-CGF mat had a nanometer-scale fiber diameter and an elastic modulus similar to skin and was able to pull during cell contraction. The PDO-CGF mat showed good biocompatibility, and the appropriate concentration of the PDO-CGF mat can significantly promote cell proliferation. ELISA showed that the PDO-CGF mat stably and continuously released TGF-β1. RT-PCR and immunofluorescence staining showed that the PDO-CGF mat stimulated fibroblasts to synthesize type I and type III collagen. These results indicate that a suitable concentration of the PDO-CGF mat can better promote the synthesis of collagen by fibroblasts.

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Section: Methodsmentioning

confidence: 99%

Promotion of skin fibroblasts collagen synthesis by polydioxanone mats combined with concentrated growth factor extracts

et al. 2019

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“…Furthermore, the membranes exhibited good properties to predict the breathing changes during sleeping in order to give an alarm for sleep apnea. A similar strategy of ion exchange from sodium to Ag ions was also used to fabricate pressure sensors by Hu et al [86]. The obtained nanofibers were integrated with planer patterned polydimethylsiloxane (PDMS) films to fabricate a wearable pressure sensor.…”

Section: Sensors and Energymentioning

confidence: 99%

Electrospun Alginate Nanofibers Toward Various Applications: A Review

et al. 2020

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Alginate has been a material of choice for a spectrum of applications, ranging from metal adsorption to wound dressing. Electrospinning has added a new dimension to polymeric materials, including alginate, which can be processed to their nanosize levels in order to afford unique nanostructured materials with fascinating properties. The resulting nanostructured materials often feature high porosity, stability, permeability, and a large surface-to-volume ratio. In the present review, recent trends on electrospun alginate nanofibers from over the past 10 years toward advanced applications are discussed. The application of electrospun alginate nanofibers in various fields such as bioremediation, scaffolds for skin tissue engineering, drug delivery, and sensors are also elucidated.

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“…The problems of enzyme leaching and poor mechanical stabilities of biomaterials have been addressed through the use of cross-linking agents such as glutaraldehyde [41,58] and epichlorohydrin [38]. Another strategy to increase the mechanical resistance is to use composite materials, which may contain different biopolymers [125][126][127], inorganic materials or magnetic nanoparticles [14].…”

Section: Pros and Cons Of Using Biopolymers To Immobilize Lipasesmentioning

confidence: 99%

Recent Trends in Biomaterials for Immobilization of Lipases for Application in Non-Conventional Media

et al. 2020

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The utilization of biomaterials as novel carrier materials for lipase immobilization has been investigated by many research groups over recent years. Biomaterials such as agarose, starch, chitin, chitosan, cellulose, and their derivatives have been extensively studied since they are non-toxic materials, can be obtained from a wide range of sources and are easy to modify, due to the high variety of functional groups on their surfaces. However, although many lipases have been immobilized on biomaterials and have shown potential for application in biocatalysis, special features are required when the biocatalyst is used in non-conventional media, for example, in organic solvents, which are required for most reactions in organic synthesis. In this article, we discuss the use of biomaterials for lipase immobilization, highlighting recent developments in the synthesis and functionalization of biomaterials using different methods. Examples of effective strategies designed to result in improved activity and stability and drawbacks of the different immobilization protocols are discussed. Furthermore, the versatility of different biocatalysts for the production of compounds of interest in organic synthesis is also described.

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A comparative study for lipase immobilization onto alginate based composite electrospun nanofibers with effective and enhanced stability

Cited by 66 publications

References 30 publications

Promotion of skin fibroblasts collagen synthesis by polydioxanone mats combined with concentrated growth factor extracts

Promotion of skin fibroblasts collagen synthesis by polydioxanone mats combined with concentrated growth factor extracts

Electrospun Alginate Nanofibers Toward Various Applications: A Review

Recent Trends in Biomaterials for Immobilization of Lipases for Application in Non-Conventional Media

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