A comparative study examining the cytotoxicity of inducible gene expression system ligands in different cell types

Xie, Jing; Nair, Ayyappan; Hermiston, Terry

doi:10.1016/j.tiv.2007.08.019

Cited by 22 publications

(20 citation statements)

References 40 publications

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“…Although Dox treatment has been reported to alter the genetic signature of a number of common cancer cell lines (36), its effect on the proliferation capacity of these cells is less significant than the marked effect observed in the present study. Furthermore, the addition of 0.2 µg/ml Dox to the culture medium in a study using Dox in gene-regulated assays demonstrated that there was no significant effect compared with the vehicle control (37).…”

Section: Resultscontrasting

confidence: 87%

Promyelocytic leukemia zinc finger triggers ATP‑binding cassette subfamily E member 1‑mediated growth inhibition in breast cancer cells

Suliman

Al-Yahya

2018

Oncol Lett

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The promyelocytic leukemia zinc finger (PLZF) protein is a transcription factor that is involved in a number of biological processes, including those regulating cellular growth; however, little is known regarding how it achieves its inhibitory effect in different cell and tissue types. It has previously been demonstrated that PLZF expression levels become diminished during the oncogenic transformation of certain tissue types and thus, may serve as a hallmark for tumor aggressiveness. To examine this in breast cancer, survival curves from available oncology databases were analyzed and demonstrated that PLZF expression was positively associated with increased survival in patients with breast cancer. The mRNA and protein levels of PLZF were also revealed to be associated with the tumorigenicity of four breast cancer cell lines. Since ATP-binding cassette subfamily E member 1 (ABCE1), also known as RNase L inhibitor, has been determined to be a target gene of PLZF, the present study also investigated whether the tumor suppressive effect of PLZF was associated with ABCE1 expression. PLZF was revealed to downregulate the expression of ABCE1 in vitro, which relieved the inhibitory effect of ABCE1 on the ribonuclease L enzyme. Finally, it was concluded that PLZF expression caused an ABCE1-mediated increase in cellular cytotoxicity, as demonstrated by a reduction in the proliferation rate of breast cancer cell lines. The results of the present study are important for understanding how PLZF exerts its final inhibitory actions in breast cancer cells, and potentially in other solid tumors, through the modulation of immunological pathways.

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Section: Resultscontrasting

confidence: 87%

Promyelocytic leukemia zinc finger triggers ATP‑binding cassette subfamily E member 1‑mediated growth inhibition in breast cancer cells

Suliman

Al-Yahya

2018

Oncol Lett

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“…35,36 Both compounds significantly inhibited the nuclear accumulation of Gfp-in compared to untreated controls (p = 0.0011 and 0.0030, respectively; fig. 5A,B).…”

Section: Mifepristone and Ivermectin Inhibit In Nuclear Import In Culmentioning

confidence: 94%

An AlphaScreen®-Based Assay for High-Throughput Screening for Specific Inhibitors of Nuclear Import

et al. 2011

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Specific viral proteins enter the nucleus of infected cells to perform essential functions, as part of the viral life cycle. The integrase (IN) molecule of human immunodeficiency virus (HIV)–1 is of particular interest in this context due to its integral role in integrating the HIV genome into that of the infected host cell. Most IN-based antiviral compounds target the IN/DNA interaction, but since IN must first enter the nucleus before it can perform these critical functions, nuclear transport of IN is also an attractive target for therapeutic intervention. Here the authors describe a novel high-throughput screening assay for identifying inhibitors of nuclear import, particularly IN, based on amplified luminescent proximity homogeneous assay (AlphaScreen®) technology, which is high throughput, requires low amounts of material, and is efficient and cost-effective. The authors use the assay to screen for specific inhibitors of the interaction between IN and its nuclear transport receptor importin α/β, successfully identifying several inhibitors of the IN/importin α/β interaction. Importantly, they demonstrate that one of the identified compounds, mifepristone, is effective in preventing active nuclear transport of IN in transfected cells and hence may represent a useful anti-HIV therapeutic. The screen also identified broad-spectrum importin α/β inhibitors such as ivermectin, which may represent useful tools for nuclear transport research in the future. The authors validate the activity and specificity of mifepristone and ivermectin in inhibiting nuclear protein import in living cells, underlining the utility of the screening approach.

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“…Ecdysteroids showed no significant toxicity in vertebrate cells [139]. Consequently, due to this benign pharmacology, ecdysteroids and nonsteroidal agonists are attractive tools for application in medicine [140].…”

Section: Gene Switchmentioning

confidence: 99%

Ecdysteroid hormone action

Spindler

Honl

Tremmel

et al. 2009

Cell. Mol. Life Sci.

103

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Several reviews devoted to various aspects of ecdysone research have been published during the last few years. Therefore, this article concentrates mainly on the considerable progress in ecdysone research observed recently, and will cover the results obtained during the last 2 years. The main emphasis is put on the molecular mode of ecdysteroid receptor-mediated hormone action. Two examples of interaction with other hormonal signalling pathways are described, namely crosstalk with juvenile hormone and insulin. Some selected, recently investigated examples of the multitude of hormonal responses are described. Finally, ecological aspects and some practical applications are discussed.

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A comparative study examining the cytotoxicity of inducible gene expression system ligands in different cell types

Cited by 22 publications

References 40 publications

Promyelocytic leukemia zinc finger triggers ATP‑binding cassette subfamily E member 1‑mediated growth inhibition in breast cancer cells

Promyelocytic leukemia zinc finger triggers ATP‑binding cassette subfamily E member 1‑mediated growth inhibition in breast cancer cells

An AlphaScreen®-Based Assay for High-Throughput Screening for Specific Inhibitors of Nuclear Import

Ecdysteroid hormone action

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