A Comparative Study between β-TCP Prepared by Solid State Reaction and by Aqueous Solution Precipitation: Application in Cements

Oliveira, Aline Pereira de; Motisuke, M.; Leal, Claudinete V.; Beppu, Marisa M.

doi:10.4028/0-87849-457-x.355

Cited by 1 publication

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“…The experimental material tested in this study corresponds to a β-TCP produced by solid-state reaction route, which showed positive mechanical results (Oliveira et al, 2008). This material has not been tested for its biocompatibility and indicators of bone formation yet.…”

Section: Discussionmentioning

confidence: 99%

“…Part of this work was associated with the Federal University of São Paulo (UNIFESP), where the experimental β-TCP was prepared and tested mechanically with satisfactory results (Oliveira et al 2008).…”

Section: Methodsmentioning

confidence: 99%

“…Some studies have shown that small changes in morphology, crystallinity, porosity, temperature, and pH of biomaterials during their manufacture can lead to different biological and mechanical behaviors of the bone substitutes (Aarvold et al, 2013;Cox, Jamshidi, Grover, & Mallick, 2014;Oliveira, Motisuke, Leal, & Beppu, 2008). Thus, the development of new bone substitutes with better physical, chemical and biological characteristics is an important requirement for evolution of medical and dental research on biomaterials.…”

Section: Introductionmentioning

confidence: 99%

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<b>Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental β-TCP compared to other bone substitutes

Milhan

Carvalho

Prado

et al. 2017

Acta Sci. Health Sci.

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ABSTRACT. The aim of this study was to evaluate the indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental beta tri-calcium phosphate (experimental β-TCP) compared with two other bone substitutes: bovine hydroxyapatite (HA) (Bio-Oss ® -Geistlich) and beta tri-calcium phosphate (β-TCP -Bionnovation). The cell viability and genotoxicity were measured by MTT and MNT assay, respectively. The indicators of osteogenesis were analyzed by alkaline phosphatase activity, total protein content, and calcium deposition. The MTT and MNT assay showed that none of the tested materials was cytotoxic nor genotoxic. Concerning the indicators of osteogenesis, it was observed that cells in contact with all the materials were able to induce the osteogenesis and this process was influenced by the period of the cell culture in contact with bone substitutes. Based on the results of this study, it was concluded that this experimental β-TCP appears to be a promising material as a bone substitute.Keywords: Beta tri-calcium phosphate, bovine hydroxyapatite.Análise dos indicadores da osteogênese, citotoxicidade e genotoxicidade de um β-TCP experimental comparado a outros substitutos ósseos RESUMO. O objetivo deste estudo foi avaliar os indicadores da osteogênese, citotoxicidade e genotoxicidade de um beta-tricálcio fosfato (β-TCP experimental) comparado com dois outros substitutos ósseos : Hidroxiapatita Bovina (HA) (Bio-Oss ® -Geistlich) e beta-tricálcio fosfato (β-TCPBionnovation). A viabilidade celular e genotoxicidade foram mensuradas pelos ensaios MTT e MNT, respectivamente. Os indicadores da osteogênese foram analisados pela atividade de fosfatase alcalina (ALP), conteúdo de proteína total, e deposição de cálcio. Os ensaios MTT e MNT mostraram que nenhum dos materiais testados foi citotóxico ou genotóxico. Em relação aos indicadores da osteogênese, foi observado que as células em contato com todos os materiais foram capazes de induzir a osteogênese, e que esse processo foi influenciado pelo período da cultura celular em contato com os substitutos ósseos. Baseado nos resultados desse estudo, conclui-se que este β-TCP experimental parece ser um material promissor para ser utilizado como substituto ósseo.Palavras-chave: Beta-tricálcio fosfato, Hidroxiapatita Bovina.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%