A Comparative Spectrophotometric Study Using Ferrozine and 1,10-Ortho-phenanthroline to Evaluate the Iron Redox Ratio (Fe2+/ΣFe) in Glass Prepared by Microwave Heating

Abstract

In the present study, Fe-doped barium borosilicate glass has been melted at 1250 C under microwave heating. The iron redox ratio (Fe 2+ /total Fe) in the glass is investigated by two spectrophotometric methods. A novel decomposition technique has been optimized to measure the ferrous oxidation state in glass. Ferrozine was chosen as a specific complexing reagent; it forms a deep violet color complex with Fe 2+ and has a broad absorbance peak centered at ~562 nm. 1,10-ortho-phenanthroline develops an orange color complex with Fe 2+ (having an absorbance peak centered at ~510 nm) and has been used to determine ferrous ion in glass. Both the methods are compared and the estimated redox ratio was found to be higher in the ferrozine method. The error limit of measurement has been determined as 0.012 and 0.023 for the ferrozine and 1,10-ortho-phenanthroline methods, respectively.

Preparation of colourless phosphate glass by stabilising higher Fe[II] in microwave heating

“…Fe 2+ -Ferrozine complex in the solution to estimate total Fe was evaluated using GC 0Sn and GM 0Sn samples for both the methods. Total iron (Fe) was estimated using method as described elsewhere 22 . Three measurements were carried out and average value at 562 nm was recorded as relative total iron concentration in the glasses.…”

Biochemical Characterization of Caenorhabditis elegans Ferritins

“…Monomer extinction coefficients of FTN-1, FTN-2, and light and heavy chain subunits of human ferritin are 17,420; 15,930; 15,930, and 19,035 M –1 cm –1 , respectively, theoretically determined from Expasy . The iron content in the protein was determined using an adapted ferrozine assay. , Ferritin samples (5 μL) were denatured by heating with 50% (v/v) sulfuric acid (10 μL) (95 °C, 10 min). The absorbance at 562 nm of the protein samples against a series of Fe­(II) standards was measured after incubating with the ferrozine reagent [30 min; ferrozine (5 mM), 2.86% (w/v) ascorbic acid and ammonium acetate (0.4 M)] using a microplate reader (FLUOstar Omega, BMG LABTECH).…”

Development of an Fe2+sensing system based on the inner filter effect between upconverting nanoparticles and ferrozine

“…1.5 mM, but this is with a high degree of uncertainty and the higher value is likely due to aggregation of particles in suspension. 50 complex. As the concentration of Fe 2+ is increased and more FZ:Fe 2+ complex is formed, the degree of quenching increases in a manner close to linear.…”