A Comparative Review of Equine SIRS, Sepsis, and Neutrophils

Sheats, M. Katie

doi:10.3389/fvets.2019.00069

Cited by 48 publications

(54 citation statements)

References 233 publications

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“…Canine in vivo work indicated that short-term oral resveratrol supplementation resulted in increased phagocytosis by PMNs, blunted oxidative burst capacity, increased PAMP-stimulated TNF and IL-6 production and no effect on IL-10 [34]. It is argued that the preserved effects of PMN phagocytosis and oxidative burst functions represent a desirable outcome in our study as those functions are a critical first line host defense to eliminate pathogens and combat infections [35].…”

Section: Discussionmentioning

confidence: 80%

Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study

Martin¹,

Johnson²,

Amorim³

et al. 2020

IJMS

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Resveratrol, a phytophenol, is a commonly used equine nutraceutical supplement touted to exert anti-inflammatory effects. The effect of orally administered resveratrol on tumor necrosis factor (TNF), interleukin-1β (IL-1β), leukocyte phagocytic activity or oxidative burst function have not been reported in horses. The objective of this study was to determine the effects of a commercially available, orally administered resveratrol product on innate immune functions in healthy adult horses. Whole blood was collected from 12 horses prior to and following 3 weeks of treatment with either the manufacturer’s recommended dose of resveratrol or placebo. Phagocytosis, oxidative burst and pathogen associated molecular pattern (PAMP) motif-stimulated leukocyte production of TNF and IL-1β were compared pre- and post-treatment between treatment groups. Phagocytosis and oxidative burst capacity were evaluated via flow cytometry. Tumor necrosis factor and IL-1β were measured using cytotoxicity and ELISA assays, respectively. There were no significant differences in phagocytosis, oxidative burst or stimulated TNF or IL-1β production between resveratrol and placebo treatment groups. Orally administered resveratrol at a routinely recommended dose for a duration of 3 weeks did not significantly affect phagocytic activity, oxidative burst function or PAMP-stimulated leukocyte cytokine production.

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Section: Discussionmentioning

confidence: 80%

Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study

Martin¹,

Johnson²,

Amorim³

et al. 2020

IJMS

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“…interleukin-6 (IL-6), serum amyloid A (SAA), soluble CD14 (sCD14) and procalcitonin (34,35). Measurement of sCD14 provided contrasting results: no signi cant differences in concentration were recorded among time in experimental settings (34), while sCD14 was higher in in spontaneous clinically endotoxemic than nonendotoxemic horses, showing however no correlation with LPS concentration (36).…”

Section: Resultsmentioning

confidence: 99%

Serial measurements of Paraoxonase-1 (PON-1) activity in horses with experimentally induced endotoxemia

Scavone

Sgorbini

Borges

et al. 2020

Preprint

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Background: Paraoxonase-1 (PON-1) is an antioxidant enzyme, whose activity decreases during the acute phase response in many species. Little is known about PON-1 and its role as a negative acute phase protein during septic inflammation in horses, but promising findings about its utility in diagnosing SIRS and predicting the outcome in diseased horses, were recently highlighted. The objective of the study was to investigate the behaviour of PON-1 in horses after experimentally induced endotoxemia. To this aim, PON-1 activity was measured on 66 plasma samples collected from six clinically healthy mares, previously included in another study, before and at multiple time points between 12 and 240 hours after intravenous infusion of Escherichia coli O55:B5 lipopolysaccharide (LPS). Results: compared with baseline values, a progressive transient decrease of PON-1 activity was observed starting from 24 hours post-infusion, with lowest values observed between 3 to 7 days post-infusion, followed by a normalisation to pre-infusion levels the tenth day.Conclusions: the results of this study suggest that measurement and monitoring of PON-1 activity might be useful to evaluate progression and recovery from endotoxemia in horses. Further studies in horses with naturally occurring sepsis are warranted.

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“…However, all the horses had at least 2 alterations of clinical parameters consistent with SIRS status evaluation proposed by Roy et al (32) and therefore it can be assumed that LPS administration induced a systemic in ammatory reaction. Biomarkers of sepsis so far evaluated in horses include interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-6 (IL-6), serum amyloid A (SAA), soluble CD14 (sCD14) and procalcitonin (34,35).…”

Section: Discussionmentioning

confidence: 99%

Serial Measurements of Paraoxonase-1 (PON-1) Activity in Horses with Experimentally Induced Endotoxemia

Scavone

Sgorbini

Borges

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Paraoxonase-1 (PON-1) is an antioxidant enzyme, whose activity decreases during the acute phase response in many species. Little is known about PON-1 and its role as a negative acute phase protein during septic inflammation in horses, but promising findings about its utility in diagnosing SIRS and predicting the outcome in diseased horses, were recently highlighted. The objective of the study was to investigate the behaviour of PON-1 in horses after experimentally induced endotoxemia. To this aim, PON-1 activity was measured on 66 plasma samples collected from six clinically healthy mares, previously included in another study, before and at multiple time points between 12 and 240 hours after intravenous infusion of Escherichia coli O55:B5 lipopolysaccharide (LPS). Results: compared with baseline values, a progressive transient decrease of PON-1 activity was observed starting from 24 hours post-infusion, with lowest values observed between 3 to 7 days post-infusion, followed by a normalisation to pre-infusion levels the tenth day. Conclusions: the results of this study suggest that measurement and monitoring of PON-1 activity might be useful to evaluate progression and recovery from endotoxemia in horses. Further studies in horses with naturally occurring sepsis are warranted.

show abstract

A Comparative Review of Equine SIRS, Sepsis, and Neutrophils

Cited by 48 publications

References 233 publications

Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study

Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study

Serial measurements of Paraoxonase-1 (PON-1) activity in horses with experimentally induced endotoxemia

Serial Measurements of Paraoxonase-1 (PON-1) Activity in Horses with Experimentally Induced Endotoxemia

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