A comparative Raman and CARS imaging study of colon tissue

Krafft, Christoph; Ramoji, Anuradha; Bielecki, Christiane; Vogler, Nadine; Meyer, Tobias; Akimov, D. A.; Rösch, Petra; Schmitt, Michael; Dietzek, Benjamin; Petersen, Iver; Stallmach, Andreas; Popp, Jürgen

doi:10.1002/jbio.200810063

Cited by 103 publications

(71 citation statements)

References 31 publications

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“…CARS hyperspectral imaging in the fingerprint region was used to image cortical bone by Pegeraro et al (in combination with SHG for collagen and TPEF for blood imaging) [237]. A comparison of spontaneous Raman and CARS imaging of brain [238] and colon tissue [239] was carried out to interpret CARS images of tissue in the high-wavenumber spectral region. The development of broad-band CARS microspectroscopy with spectral reconstruction demonstrated by Camp et al was applied to ex vivo tissue imaging of liver tissue from mice [215].…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

View full text Add to dashboard Cite

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“…Aside from lipids other relevant molecules yield good CARS image contrast, e.g. DNA or proteins [49,50]. Another equally powerful NLO imaging technique providing morpho-functional information on biological tissues is represented by second-harmonic generation (SHG) microscopy [3,51].…”

Section: Introductionmentioning

confidence: 99%

From molecular structure to tissue architecture: collagen organization probed by SHG microscopy

Cicchi

Vogler

Kapsokalyvas

et al. 2012

Journal of Biophotonics

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170

132

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Second‐harmonic generation (SHG) microscopy is a fantastic tool for imaging collagen and probing its hierarchical organization from molecular scale up to tissue architectural level. In fact, SHG combines the advantages of a non‐linear microscopy approach with a coherent modality able to probe molecular organization. In this manuscript we review the physical concepts describing SHG from collagen, highlighting how this optical process allows to probe structures ranging from molecular sizes to tissue architecture, through image pattern analysis and scoring methods. Starting from the description of the most relevant approaches employing SHG polarization anisotropy and forward – backward SHG detection, we then focus on the most relevant methods for imaging and characterizing collagen organization in tissues through image pattern analysis methods, highlighting advantages and limitations of the methods applied to tissue imaging and to potential clinical applications. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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“…Furthermore, MALDI-TOF constitutes a destructive technique; therefore, MALDI-TOF will not be helpful imaging dynamical changes. A technique that is in principle capable of imaging the wax distribution in living leaves is Raman-based microscopy (Yu et al, 2008;Krafft et al, 2009aKrafft et al, , 2009bWalter et al, 2010). Raman scattering provides chemical fingerprint information, can be recorded in back-scattering direction (epidetection), and is generally not hampered by the presence of water in biological samples (Urlaub et al, 1998).…”

mentioning

confidence: 99%

Toward in Vivo Chemical Imaging of Epicuticular Waxes

Weissflog

Vogler²,

Akimov³

et al. 2010

Plant Physiology

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Epicuticular waxes, which are found on the outer surface of plant cuticles, are difficult to study in vivo. To monitor the growth, development, and structural alterations of epicuticular wax layers, coherent anti-Stokes Raman scattering (CARS) might be used. CARS, as a Raman-based technique, not only provides structural insight but also chemical information by imaging the spatial distribution of Raman-active vibrations. Here, we present a comparative study using CARS and scanning electron microscopy to characterize the structure of epicuticular waxes. The ability of CARS to provide detailed structural information on the biologically important wax layer was detailed on the examples of cherry laurel (Prunus laurocerasus), hoya (Hoya carnosa), and ceriman/Swiss cheese plant (Monstera sp. aff. deliciosa). We anticipate that the work presented will open a doorway for online monitoring of formation and alterations of epicuticular wax layers.

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A comparative Raman and CARS imaging study of colon tissue

Cited by 103 publications

References 31 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

From molecular structure to tissue architecture: collagen organization probed by SHG microscopy

Toward in Vivo Chemical Imaging of Epicuticular Waxes

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