A comparative radiation hybrid map of bovine chromosome 18 and homologous chromosomes in human and mice

Goldammer, Tom; Kata, Srinivas R.; Brunner, Ronald M.; Dorroch, Ute; Sanftleben, Hanka; Schwerin, Manfred; Womack, James E.

doi:10.1073/pnas.042688699

Cited by 30 publications

(18 citation statements)

References 44 publications

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“…The QTL effect for udder depth at position 30 cM could be due to the correlation of this trait with SCS (LUND et al, 1994). Several studies (e.g., GOLDAMMER et al, 2002;BRUNNER et al, 2003) have reported that the telomeric region of BTA18 exhibits high similarity to the HSA19q region in humans, that contains several gene families with effect on the immune system such as the SIGLEC-(sialic acid binding Ig-like lectin), the KIR-(killer cell immunoglobulin-like receptor) and the LILR (leukocyte immunoglobulin-like receptor) gene clusters. For the peak at 34 cM, there are a lot of these above mentioned genes possibly suitable as candidates for further studies.…”

Section: Resultsmentioning

confidence: 99%

Confirmation of quantitative trait loci for somatic cell score on bovine chromosome 18 in the German Holstein

Paul

Bennewitz

et al. 2006

Arch. Anim. Breed.

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Fifty-one half-sib families with 2768 sires were selected for this study to confirm a quantitative trait locus (QTL) detected within a previous project initiated by the German Cattle Breeders Federation (ADR). The data based on a granddaughter design were divided into two parts and were analysed using linear models and paternal half sib regression methods. The results strongly support the hypothesis that the chromosomal region around marker TGLA227 at the telomeric end of chromosome 18 harbours a QTL for somatic cell score (SCS) in the German Holstein population. Using a two-QTL model the analysis showed evidence for another QTL for SCS in that region on chromosome 18. Further fine mapping studies should be carried out to decide on the two QTL hypothesis.

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Section: Resultsmentioning

confidence: 99%

Confirmation of quantitative trait loci for somatic cell score on bovine chromosome 18 in the German Holstein

Paul

Bennewitz

et al. 2006

Arch. Anim. Breed.

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“…BTA18 has the largest number of mapped markers (n = 121), whereas BTA28 has the fewest (n = 23). Relative differences in marker distribution are due in part to targeting of specific chromosomes for high-density mapping (e.g., BTA18; Goldammer et al 2002) as well as chromosome size. The average chromosome length is 397 cR 5000 , ranging from 563 cR for BTA16 to 150 cR for BTA20 (Table 1).…”

Section: Resultsmentioning

confidence: 99%

A 1463 Gene Cattle–Human Comparative Map With Anchor Points Defined by Human Genome Sequence Coordinates

Wind¹,

Kata²,

Band³

et al. 2004

Genome Res.

Self Cite

139

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A second-generation 5000 rad radiation hybrid (RH) map of the cattle genome was constructed primarily using cattle ESTs that were targeted to gaps in the existing cattle–human comparative map, as well as to sparsely populated map intervals. A total of 870 targeted markers were added, bringing the number of markers mapped on the RH5000 panel to 1913. Of these, 1463 have significant BLASTN hits (E < e–5) against the human genome sequence. A cattle–human comparative map was created using human genome sequence coordinates of the paired orthologs. One-hundred and ninety-five conserved segments (defined by two or more genes) were identified between the cattle and human genomes, of which 31 are newly discovered and 34 were extended singletons on the first-generation map. The new map represents an improvement of 20% genome-wide comparative coverage compared with the first-generation map. Analysis of gene content within human genome regions where there are gaps in the comparative map revealed gaps with both significantly greater and significantly lower gene content. The new, more detailed cattle–human comparative map provides an improved resource for the analysis of mammalian chromosome evolution, the identification of candidate genes for economically important traits, and for proper alignment of sequence contigs on cattle chromosomes.

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“…PCR typing of TLR9 was performed in a cattle-mouse somatic-hybrid cell (SHC) panel (55), and in a cattle-hamster 5,000-rad wholegenome radiation hybrid panel WGRH 5000 (54), essentially as described previously (18). The WGRH typing experiment was performed twice, and data were scored independently.…”

Section: Methodsmentioning

confidence: 99%

Mastitis Increases Mammary mRNA Abundance of β-Defensin 5, Toll-Like-Receptor 2 (TLR2), and TLR4 but Not TLR9 in Cattle

Goldammer

Zerbe

Molenaar

et al. 2004

Clin Vaccine Immunol

245

172

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Coordination of the primary defense mechanisms against pathogens relies on the appropriate expression of pathogen recognition receptors (PRRs) triggering the early release of effector molecules of the innate immune system. To analyze the impact of this system on the counteraction of infections of the mammary gland (mastitis), we characterized the bovine gene encoding the key PRR Toll-like receptor 9 (TLR9) and mapped its precise position on chromosome BTA22. The sequence information was used to establish real-time PCR quantification assays to measure the mRNA abundances of TLR9, TLR2, and TLR4 together with those of ␤-defensin 5 (BNBD5), an early bactericidal effector molecule of the innate system, in healthy and infected mammary glands. Mastitis strongly increased (4-to 13-fold) the mRNA abundances of all of these genes except TLR9. Slight subclinical infections already caused a substantial increase in the copy numbers, though they did so the least for TLR9. Induction was not systemic, since mRNA abundance was low in uninfected control quarters of the udder but high in the severely infected quarters of the same animal. The number of TLR2 copies correlated well with those of TLR4, indicating coordinated regulation of these two PRRs during infection of the udder. Their coordinated regulation explains our unexpected observation that pure Staphylococcus aureus infections caused a strong increase also in TLR4 mRNA abundance. In situ hybridizations revealed that BNBD5 is expressed predominantly in the mammary epithelial cells (MEC) of the infected gland. Our data therefore suggest a significant contribution of the innate immune system to counteract mastitis and attribute a prominent effector function to the MEC.

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A comparative radiation hybrid map of bovine chromosome 18 and homologous chromosomes in human and mice

Cited by 30 publications

References 44 publications

Confirmation of quantitative trait loci for somatic cell score on bovine chromosome 18 in the German Holstein

Confirmation of quantitative trait loci for somatic cell score on bovine chromosome 18 in the German Holstein

A 1463 Gene Cattle–Human Comparative Map With Anchor Points Defined by Human Genome Sequence Coordinates

Mastitis Increases Mammary mRNA Abundance of β-Defensin 5, Toll-Like-Receptor 2 (TLR2), and TLR4 but Not TLR9 in Cattle

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